Molecular Detection and Pathogenic Capability of Agrobacterium tumefaciens Isolates from Olive | ||||
| Alexandria Science Exchange Journal | ||||
| Article 17, Volume 39, July-September - Serial Number 3, July 2018, Page 521-526 PDF (287.25 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asejaiqjsae.2018.16883 | ||||
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| Authors | ||||
| Nagia M. Jadalla* 1; Said I. Behiry* 2; Mohamed A.M. Adam* 1 | ||||
| 1Plant protection Department, Faculty of Agriculture -Omar Al-mukhtar University. Elbida-Libya | ||||
| 2Agricultural Botany Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt | ||||
| Abstract | ||||
| Crown gall, caused by Agrobacterium tumefaciens, first reported on olive in Libya. A partial 16SrRNA gene sequence confirmed the identity of the A. tumefaciens bacterial isolates. The Ti-plasmid (induce tumors formation) is the real reason of their pathogenicity. Meanwhile, the prerequisite for the control of A. tumefaciens is to have a rapid and reliable detection method of pathogenic isolates in order to select healthy plants therefore, we detected two plasmidic genes, virD2 gene of the virulence (vir) region (amplified by virD2A/C and virD2A/E primers), and T-DNA (on tms gene). The PCR amplified bands analysis had a correspondence to the pathogenicity assay (carrot tumorgenicity) and the biochemical determents’ of the isolates genus. | ||||
| Keywords | ||||
| Agrobacterium tumefaciens; PCR; Ti-plasmid; virD2; pathogenicity | ||||
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