Hypoxia and Oxidative Stress Biomarkers in Hemodialysis Pediatric Patients with Chronic Renal Failure | ||||
| GEGET | ||||
| Article 8, Volume 11, Issue 1, December 2011, Page 97-109 PDF (1.32 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/geget.2011.16894 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ahmad Ahmad1; Enas named2; Taghrid El-Abaseri3; Amany Mohamed4; Tarek EІ-МеtwаlІу5 | ||||
| 1Faculties of Medicine, Departments of Pediatrics, Assiut University, Assiut; and Suez Canal University* , Isтailia, Egypt. | ||||
| 2Faculties of Medicine, Departments of Pediatrics, Medical Physiolof, Assiut University, Assiut; and Suez Canal University* , Isтailia, Egypt. | ||||
| 3Faculties of Medicine ,Medical Biochemistry Assiut University, Assiut; and Suez Canal University* , Isтailia, Egypt. | ||||
| 4Faculties of Medicine, Departments of Medical Biochemistry ,Assiut University, Assiut | ||||
| 5Faculties of Medicine, Departments of Pediatrics, Medical Physiolof, and Suez Canal University , Isтailia, Egypt. | ||||
| Abstract | ||||
| Background: Chronic renal failure (CRF) is responsible for high morbidity and mortality. Tissue injury due to hypoxia and/or free radicals is common in a variety of disease processes. Objectives: This prospective study aimed to investigate effect of CRF and hemodialysis (HD) on hypoxia and oxidative stress biomarkers. Methods: Forty pediatric patients with CRF on HD and 20 healthy children were recruited. Plasma hypoxia induced factor-la (HIF-la), vascular endothelial growth factor (VLGF) were measured by ELISA kits. Total antioxidant capacity (TAC). total peroxide (TPX). pyruvate and lactate by chemical colorimetric methods. Oxidative stress index (OSI) and lactate/pyruvate (L/P) ratio were calculated. Results: TAC was significantly lower while TPX, OSI, VEGF were higher in patients with pre- and post-dialysis session than controls. Lactate, HIF-la levels were significantly higher in pre-dialysis session than controls. Before dialysis, l4'( and LIP ratio were lower than post-dialysis. In controls, LIP ratio correlated positively with ТАС; HIF-le correlated positively with each of LIP ratio and ТАС; VEGF correlated positively with each of TPX and OSI. In post-dialysis, IIF-la correlated negatively with each of ТРХ and OSI. In pre-dialysis session, HIF-la correlated positively with each of dialysis duration and plasma creatinine: VEGF correlated positively with each of dialysis duration, pyruvate and HIF-la, but, negatively with РО,; and, OSI correlated positively with "ГРХ, but, negatively with each of TAC and hemoglobin. Conclusion: CRF patients to succumb considerable hypoxia with oxidative stress Hemodialysis ameliorated hypoxia but lowered antioxidants as evidenced by decreased levels of HIF-la and TAC in pre- compared to post-dialysis levels. | ||||
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