Antibacterial Potential of Pomegranate Peel Extracts on Escherichia coli Isolated From Benha Hospital in Egypt | ||||
Benha Journal of Applied Sciences | ||||
Article 11, Volume 6, Issue 3, May and June 2021, Page 61-64 PDF (232.27 K) | ||||
Document Type: Original Research Papers | ||||
DOI: 10.21608/bjas.2021.169813 | ||||
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Authors | ||||
S.H. Abdel-Aziz1; M.A. El-Esawi2; M.M. Hazaa1; Hadeer Y. Abdel-Aziz1; Mervat G. Hassan1 | ||||
1Botany and Microbiology, Dept., Faculty of Science, Benha Univ., Benha, Egypt | ||||
2Botany Dept., Faculty of Science, Tanta Univ., Tanta, Egypt | ||||
Abstract | ||||
Introduction. Pomegranate is an old fruit which has many antimicrobial compounds. Impact of pomegranate peel extracts on Escherichia coli clinical isolate from Benha general hospital was investigated in this study. Material and Methods: pomegranate peel extraction was primed and GC/MS chromatogram analysis was carried out using the GC Agilent Technologies gas chromatography fitted with HP-5 fused silica column (25mm × 0.25mm, film thickness of 0.25 µm) and interfaced to the flame-ionization detector (FID). The antibacterial potential of Pomegranate peel extractions against E.coli sample was done using well diffusion method. Results: According to (GC/MS) technique, methanol and aqueous extracts of pomegranate peel have inhibitory effects on E. coli isolate. The minimum inhibition concentrations (MICs) of methanolic and aqueous extracts were 100 and 60 mg/L, respectively. In this study , the MICs values of Aqueous extract of pomegranate peel were higher than of methanolic extract of pomegranate peel (P<0.05). Zones of inhibition and values of MICs indicated that the aqueous extract of pomegranate peel had more effect on E. coli isolate than methanolic extract of pomegranate peel. Conclusion: In this study, the extraction of pomegranate peel has antibacterial effect on E.coli isolate. However, the aqueous extract had a higher effect on E.coli than the methanolic extract. | ||||
Keywords | ||||
E.Coli; pomegranate peel; ESBLs | ||||
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