Effects of Two Neonicotinoids Insecticides on Some Anti-Oxidant Enzymes and Hematological Parameters in Egyptian Frogs, Bufo regularis. | ||||
| Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control | ||||
| Article 3, Volume 10, Issue 1, June 2018, Page 25-36 PDF (351.94 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsf.2018.17017 | ||||
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| Authors | ||||
| Naglaa M. Loutfy; Marwa S. Kamel | ||||
| Department of Plant Protection, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt | ||||
| Abstract | ||||
| The effect of two neonicotinoid pesticides, Actra and Acetamore, on the Egyptian frog (Bufo regularis) was evaluated. Some parameters were selected to reflect and measure the stress caused by exposing frogs to 1/6 of the recommended field concentration. Stress parameters included malondialdehyde (MDA), the antioxidant enzyme, reduced glutathione (GSH) and superoxide dismutase (SOD) besides some hematological parameters. Results showed that activities of the enzymes GSH and SOD decreased significantly after treatments with both pesticides. GSH content decreased by 29.47% and 47.37% compared to control frogs, while SOD activity decreased by 11.37% and 18.88% after exposure to Actra and Acetamore pesticides, respectively. The exposure for both pesticides promotes significant increase in MDA content and also caused a significant reduction for serum albumin fraction and total proteins. The number of total red blood cells (RBCs) and hematocrit values showed a non-significant decrease, whereas the number of white blood cells (WBCs) increased significantly by 82.54% and 127.5%, after the exposure to Actra and Acetamore, respectively. Hemoglobin content and the blood indices such as Mean corpuscular volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC) declined significantly only in Acetamore treated frogs. Changes caused by Acetamore exposure were much more profound for nearly all studied parameters in comparison to changes caused by Actra. Alteration took place in the studied parameters could have adverse influence on major physiological functions in frogs, which could result in more vulnerable adults. | ||||
| Keywords | ||||
| Frogs; Antioxidant enzymes; hematology; neonicotinoids pesticides | ||||
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