Appraisal of Positive Pesticides Influence on Pink Bollworm Larvae, Pectinophora gossypiella (Saunders) | ||||
| Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control | ||||
| Article 4, Volume 10, Issue 1, June 2018, Page 37-47 PDF (348.9 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsf.2018.17018 | ||||
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| Authors | ||||
| Eman M. M. Radwan1; Mohamed A. El-Malla1; Mohamed A. Fouda2; Ragab A. Soliman Mesbah1 | ||||
| 1Agricultural Research Center, Central Agricultural Pesticides Laboratory, Ministry of Agriculture, Dokki, Giza | ||||
| 2Faculty of Science, Department of Zoology, Al-Azhar University, Nasr City, Cairo, Egypt | ||||
| Abstract | ||||
| Experiments conducted to study the toxic and biological effects of three chemical insecticides (chlorpyrifos, Lambda-Cyhalothrin, and Methomyl), one biochemical insecticide (spinosad), and two bio-insecticides (Bacillus thuringiensis kurstaki Bt and Nucleopolyhedrovirus, NPV) against neonate larvae of the pink bollworm, Pectinophora gossypiella (Saund.), were carried out under laboratory conditions. The highest effect of tested pesticides on larval mortalities was recorded after four days of feeding these larvae on treated diet. Spinosad was the effective toxin (LC50=1.84 ppm) to neonate larvae followed by chlorpyrifos (LC50= 3.06 ppm), Lambda-Cyhalothrin (LC50= 5.99 ppm) then Methomyl (LC50=23.50 ppm) but B.t. (74.84×104 I.U) and NPV (83.5×109 I.U) were the lowest ones. LC50 treatments of neonate larvae with all tested insecticides produced reduction in larval and pupal weights especially Lambda-cyhalothrin treatment, (31.9 and 24.3 %, resp.) compared with untreated insects. The decreasing effect of all treatments observed in fecundity and life span of adult females which reflected in very high percentages of insect sterility with chlorpyrifos (66.7%) and Methomyl (61%) treatments. Insignificant increase of total protein contents and activity of Glutathione-S-transferase enzyme was detected in tissues of LC50 treated larvae compared with untreated larvae, but a significant (23.5%) and highly significant (56.9%) increase in enzyme activity were resulted by Chlorpyrifos and Spinosad treatments. A very high inhibition in activity of Acetylcholinesterase enzyme of larval tissues was recorded with NPV (61.3%), B.t. (58.8%), Lambda-Cyhalothrin (55.6%), Spinosad (54.4%), Chlorpyrifos (48.7%), and Methomyl (37.5%) treatments than untreated ones. | ||||
| Keywords | ||||
| pink bollworm; Chemical pesticides; biochemical insecticides; bio-insecticides; biological aspects; GST and AchE enzymes | ||||
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