Microbial Control on Sesamia cretica Insects by Beauveria bassiana at Sharkia Governorate | ||||
| Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control | ||||
| Article 4, Volume 9, Issue 3, December 2017, Page 31-46 PDF (2.97 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsf.2017.17026 | ||||
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| Authors | ||||
| Sherief E. A. H.1; Ghaly M. F.2; El-Mougith A. A. A.2; Abd El- Kader M. A.1 | ||||
| 1Department of Botany, Faculty of Science, Zagazig University, Egypt | ||||
| 2Plant Protection Research Institute, Sharkia, Egypt | ||||
| Abstract | ||||
| This research was performed in Sharkia Governorate during seasons of 2014and 2015 under laboratory conditions. Beauveria bassiana (Blas.) is an imperfect entomopathogenic fungus that attacks a wide range of agriculture pests causing disease named as white muscardine and currently used as biocontrol agents and substitute the harmful chemical insecticides. Beauveria bassiana isolate (Cairo MIRCEN) was evaluated as biocontrol agent against Sesamia cretica Led. under laboratory conditions. B. bassiana caused 100% mortality to S. cretica after 5 days. B. bassiana was screened for lytic enzyme production as it had the ability to produce chitinase, protease and no lipase production. Environmental and nutritional conditions were studied to detect the optimum conditions for growth, protease production. Fungal isolate was identified by both microscopic conditions. Culture filtrate of B. bassiana 1572 become concentrated (partial purified active compound by organic solvents (chloroform)) and tested on insects Sesamia cretica (larva at different ages) as bioinsecticide in comparison with diluted (original filtrate). Results were indicated after microscopic examinations as a complete change in insect body colour to be deep darker, Appearance of white growth of the fungal B. bassiana 1572 on the treated dead insect body in both treatments and the insect cadaver was solidified and there were some black lesions and some malformation. All symptoms were more in concentrated treatment than the other treatment.Also beauvericin toxin production was investigated by molecular weight using SDS- Protein Electrophoresis that indicated the presence of beauvericin toxin in both concentrated (partial purified active compound by organic solvents (chloroform)) and diluted (original filtrate). When conidia of B. bassiana 1572 became in contact with the insect cuticle surface under suitable ecological condition, it germinate, by the aid of both chemical (lytic enzyme secretion (chitinase, protease)) and mechanical effect (hyphal pressure on the penetration site. Then, hyphae penetrate the insect body cavity. That led to hyphae growth, division, beauvericin toxin production and production of spores spreaded in the hemolymph (in all parts of body cavity), after that physical and chemical changes occurred to the insect hemolymph by the aid of beauvericin toxin led to function disorder and death. The fungus grew outside the cadaver until the availability of suitable conditions to resume life cycle. | ||||
| Keywords | ||||
| Microbial control – Sesamia cretica –Beauveria bassiama – Sharkia-Governorate | ||||
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