Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia at Schiphra Hospital of Ouagadougou in Burkina Faso
Kaboré, B., CISSE, H., Zongo, K., Sanou, I., Zeba, B., Traoré, Y., SAVADOGO, A. (2022). Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia at Schiphra Hospital of Ouagadougou in Burkina Faso. EKB Journal Management System, 3(1), 128-134. doi: 10.21608/mid.2021.70446.1138
Boukaré Kaboré; Hama CISSE; Koudbi Jacob Zongo; Idrissa Sanou; Boukaré Zeba; Yves Traoré; Aly SAVADOGO. "Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia at Schiphra Hospital of Ouagadougou in Burkina Faso". EKB Journal Management System, 3, 1, 2022, 128-134. doi: 10.21608/mid.2021.70446.1138
Kaboré, B., CISSE, H., Zongo, K., Sanou, I., Zeba, B., Traoré, Y., SAVADOGO, A. (2022). 'Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia at Schiphra Hospital of Ouagadougou in Burkina Faso', EKB Journal Management System, 3(1), pp. 128-134. doi: 10.21608/mid.2021.70446.1138
Kaboré, B., CISSE, H., Zongo, K., Sanou, I., Zeba, B., Traoré, Y., SAVADOGO, A. Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia at Schiphra Hospital of Ouagadougou in Burkina Faso. EKB Journal Management System, 2022; 3(1): 128-134. doi: 10.21608/mid.2021.70446.1138

Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia at Schiphra Hospital of Ouagadougou in Burkina Faso

Microbes and Infectious Diseases
Article 15, Volume 3, Issue 1, February 2022, Page 128-134  XML PDF (377.58 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.70446.1138
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Authors
Boukaré Kaboré1; Hama CISSEorcid 1; Koudbi Jacob Zongo2; Idrissa Sanou3; Boukaré Zeba1; Yves Traoréorcid 1; Aly SAVADOGO email orcid 1
1Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03
2Department of Biochemistry and Microbiology, Faculty of Applied Science and Technology, Burkina Faso, BP 176 DEDOUGOU
3UFR Health Sciences, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03. Laboratory of Bacteriology and Virology at Tengadogo University Hospital, Ouagadougou, Burkina Faso, BP 104 Ouagadougou
Abstract
Background: Epidemic of carbapenemase-producing bacteria has become worldwide. Thus, during the last decade, the prevalence of carbapenem-resistant Enterobacteriaceae and non fermentative Gram-negative bacteria in human patients have increased. Carbapenemase-producing bacteria are usually multidrug resistant. Therefore, early recognition of carbapenemase producers is critical to prevent their spread. Objectives: The aim of this study was to contribute establishing the prevalence of isolates producing Metallo-β-Lactamase isolated from patients admitted to Schiphra Hospital of Ouagadougou. Methods: Susceptibility of bacteria to antimicrobial agents was evaluated by disc diffusion method using imipenem as screening antibiotic. The combination of imipenem-EDTA was used after detection the resistance to imipenem. Results: A total of 52 isolates resisting one of the third generation cephalosporins were collected. Five isolates showed an intermediary resistance to imipenem (9.61%). Two isolates were resistant to imipenem-EDTA (3.85%). The test of imipenem-EDTA was done to confirm to production of Metallo-β-Lactamase. The hydrolysis of bacterial extract by meropenem was confirmed by production with a kinetic activity at spectrophotometer V0=4.77x10–5µM/min for Pseudomonas aeruginosa and V0=1.183x10-4 µM/min for Stenotrophomonas maltophilia. Conclusion: This study showed that bacterial resistances by production to metallo-β-lactamases are a reality in Burkina Faso.
Keywords
Carbapenem; Imipenem-EDTA; Meropenem; Metallo-β-lactamases; Burkina Faso
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