USE OF SEROLOGY, SDS-PAGE, AND RAPD ANALYSIS TO EVALUATE RESISTANCE OF FLAX TO POWDERY MILDEW | ||||
Egyptian Journal of Agricultural Research | ||||
Article 2, Volume 89, Issue 1, March 2011, Page 17-34 PDF (703.87 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejar.2011.170370 | ||||
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Authors | ||||
EZAT M. HUSSEIN1; MAHMOUD T. MANSOUR1; MAGGIE E.M. HASSAN1; EMAN A. EL-KADY2; KHALED K. KASEM1 | ||||
1Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. | ||||
2Field Crops Research Institute, Agricultural Research Center, Giza, Egypt. | ||||
Abstract | ||||
Eight flax cultivar were evaluated for powdery mildew resistance under field conditions , C.I. 2008, Cortland, Linore and the local cultivars Giza 8 , Giza 7 were highly susceptible. Disease severities on these cultivars were 100.00, 99.48 and 95.26 , 93.46 and 87.26%respectively. On the other hand, cultivars Williston brown, Wilden and Dokota were highly resistant,with disease severities on these cultivars were 27.58, 25.11 and 19.67%, respectively. SDS-PAGE, RAPD analysis by using four primers and double diffusion test (DDT) were used to differentiate between resistance and susceptible genotypes. RAPD analysis by using primer no. 6 and DDT were able, at least partially, to differentiate between resistant and susceptible genotypes, while SDS-PAGE was unable to differentiate between cultivars of the two groups. | ||||
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