RESPONSE OF CUCUMBER AND OKRA TO COMBINED APPLICATION OF AZOTOBACTER TRANSFORMANTS AND CHEMICAL NITROGEN FERTILIZER | ||||
| Journal of Plant Production | ||||
| Article 14, Volume 33, Issue 8, August 2008, Page 5935-5948 PDF (617.19 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2008.170395 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| E. A. Hassan1; Manal A. Abd Allah1; K. A. Abd Elaziz1; O. F. Dakhly2 | ||||
| 1Veg. Res. Dept., Hort. Res. Inst. Agric. Centre, Ciza, Egypt, | ||||
| 2Genetic Dept., Fac., of Agric. Minia University | ||||
| Abstract | ||||
| The present investigation was carried out (Lab. study) in microbial genetics lab., Genetic Dept., Minia University and a field study was carried out at Sakha agriculture research station farm during the two successive summer season of 2004 and 2005. DNA extract from Bacillus sp < /em>. (grown under 20% NaCl) was used transform salt isolate to sensitive isolates of Azotobacter vinelandii Wild type. The producing transformants were used to infect cultivars of cucumber (hybrid prince) and Okra Eskandrandi). Results showed that the highest frequencies of transformants (330 x 10-6) was obtained on 10% NaCl at 24 hours. Results also, indicated that there were significant differences in growth characteristics between the plants inoculated with the tested strains when compared with the control plants (non-inoculated) or the plants inoculated with the wild type strain. All transformants in our study had the best effect on most growth characters and yield as compared with wild type strain. The best transformants was T-4 which had the highest percentage of increasing total yield with both crops. The data show that there were differences among both, methods of incubation (Liquid or capsules) in their effects on the two crops in their response. The best method was liquid which had the highest values of all growth characters and total yield. Finally, the present investigation recommends the importance of inoculating vegetables crops with transformants of A. vinelandii to improve their growth and yield with using the liquid method. | ||||
| Keywords | ||||
| Okra, cucumber, (Cucumis sativus, L); Azotobacter vinelandii, Azotobacter transformants | ||||
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