STUDIES ON PROMOTER EXPRESSION OF TOXINS GENES IN SOME FOOD POISONING BACTERIA. | ||||
| Menoufia Journal of Agricultural Biotechnology | ||||
| Article 1, Volume 5, Issue 3, May 2020, Page 101-102 PDF (224.4 K) | ||||
| Document Type: original papers | ||||
| DOI: 10.21608/mjab.2020.170539 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Wafaa H. Mahmoud1; A. E. Elbeltagy1; S. F. F. Allah2 | ||||
| 1Prof. of Microbiology, Fac. of Agric., Menoufia Univ. | ||||
| 2Associate Prof. of Microbiology, Fac. of Agric., Menoufia Univ. | ||||
| Abstract | ||||
| This work provides the evidence that considerable increase in Salmonella activity takes place within 24 h at ambient and above room temp up to 40°C. The temperature range for the growth of Salmonella spp. is 5 to 40°C, where the optimal temperature range lies in between 35 and 40°C. Exposure to high temperature, Salmonella Typhimurium serovar was found to be more sensitive. We provided the evidence that concentration of Salmonella total RNA indicates its preparedness in the form of metabolic and cellular activities to cope with environmental stress while in contact with epithelial cells (IEC-6) led to an increase in the production of toxin. Relative expression of temperature of toxin gene and virulent genes of Salmonella reveals both in terms of activation and repression of target genes in diverse expression modes depending on the cellular activity. Salmonella Typhimurium seems to be more potent and showed increased ability to induce the expression of enterotoxin gene (stn) by the exposure of temperature elevation. | ||||
| Keywords | ||||
| Salmonella; virulent genes; invA gene; avrA gene; enterotoxin gene (stn) | ||||
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