Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response
Abd El-Hady, A., Kamel, W., Farid, M., Sabry, D. (2021). Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response. EKB Journal Management System, 8(2-A), 231-241. doi: 10.21608/adjg.2021.28468.1246
Asmaa Abd El-Hady; Wael H. Kamel; Mona H. Farid; Dina Sabry. "Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response". EKB Journal Management System, 8, 2-A, 2021, 231-241. doi: 10.21608/adjg.2021.28468.1246
Abd El-Hady, A., Kamel, W., Farid, M., Sabry, D. (2021). 'Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response', EKB Journal Management System, 8(2-A), pp. 231-241. doi: 10.21608/adjg.2021.28468.1246
Abd El-Hady, A., Kamel, W., Farid, M., Sabry, D. Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response. EKB Journal Management System, 2021; 8(2-A): 231-241. doi: 10.21608/adjg.2021.28468.1246

Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response

Al-Azhar Dental Journal for Girls
Article 8, Volume 8, 2-A, April 2021, Page 231-241  XML PDF (1.04 MB)
Document Type: Original Article
DOI: 10.21608/adjg.2021.28468.1246
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Authors
Asmaa Abd El-Hady email orcid 1; Wael H. Kamel2; Mona H. Farid3; Dina Sabryorcid 4
1Assistant Lecturer of Endodontics, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
2Professor and Head of Endodontic Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
3Professor of oral and dental biology, Former Dean of the Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
4Professor of medical biochemistry and molecular biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Abstract
Purpose: To study the effect of Chitosan final irrigating solution compared with EDTA using different irrigation techniques, on TGF-β1 release from root canal dentin, using enzyme-linked immunosorbent assay (ELISA), and to evaluate the effect of released TGF-β1 on Dental Pulp Stem Cells. Materials and Methods: Human DPSCs were isolated from impacted third molars. Forty eight human root segments were prepared to a standardized truncated cone-shaped canal with an open apex of 1 mm diameter, the volume of prepared segments were determined using CBCT. Samples were randomized into two experimental groups (n=24) regarding the final irrigating solution; Group I: 1.5% NaOCl followed by 17% EDTA, Group II: 1.5% NaOCl followed by 1% Chitosan, then subdivided into subgroups A and B (n = 12) according to the final irrigation technique: A: Needle Irrigation, B: XP-Endo Finisher agitation. The released TGF-β1 were quantified using ELISA at 4 hours, 1 day, and 3 days following irrigation. DPSCs response to1.5% NaOCl, 17% EDTA, 1% Chitosan, and different concentrations of released TGF- β1 was assessed using MTT assay.
Results: No statistically significant difference in the quantity of TGF-β1 released by 17% EDTA and 1% Chitosan following needle irrigation or XP Endo Finisher agitation. 1% Chitosan significantly increased DPSCs viability compared to17% EDTA (P < 0.05). TGF-β1 showed significant expansion of viable DPSCs in a positive correlation to its concentration (P <0.001). Conclusions: 1% Chitosan effectively released TGF-β1 from root canal dentin as17% EDTA. The released TGF-β1 promoted DPSCs proliferation at picogram levels in a concentration dependent manner.
Keywords
Chitosan; TGF-β1; Dental Pulp Stem Cells
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