IN VITRO CULTURE OF IMMATURE EMBERYOS AND ANTHER CULTURES OF BREAD WHEAT | ||||
| Journal of Plant Production | ||||
| Article 1, Volume 33, Issue 10, October 2008, Page 6985-6994 PDF (663 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2008.171216 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| I. E. El-Beially* | ||||
| Agronomy Dept, Faculty of Agric. Al-Azhar University at Cairo | ||||
| Abstract | ||||
| This investigation was carried out at the Cell and Tissue Culture Lab. Agronomy Dept. Faculty of Agriculture , Al-Azhar University, during the period from 2005 to 2006 aiming to study the main factors affecting callus Induction and plant regeneration from cultured immature embryos of cultivars of wheat (Triticum aestivum L) namely Gemmeiza 7, Gemmeiza 9 , Gemmeiza 10, Giza 168 and Sakha 93 .Experiments were designed to identify cultivars of wheat and growth regulator most suitablefor successful in vitro immature embryos and anther culture stages were also investigated. Results indicated that there were significant effect for the cultivars , growth regulators and their interaction on callus induction from the immature embryos. The cultivar G168 recorded the highest significant effect for callus induction followed by Gemmeiza 10. The highest value of callus initiation recorded when 2.0 mg/L2,4-D+0.5 mg/L kin was added to the MS medium. Results indicated that there were significant effect for growth regulators and their interaction with genotypes on plant regeneration. Growth regulators, effected on the plant regeneration percent. The highest value of plant regeneration recorded when 1.0 mg/L kin +0.5 mg /L 2,4-D or 1.0 mg /L BAP +0.5 mg /L NAA were added to the MS medium. The interaction between cultivars and growth regulators was significant . The highest plant regeneration recorded for Gemmeiza 10 cultured on MS medium supplemented with 1.0 mg /L BAP +0.5 mg /L NAA. For anther studies the highest mean for plant regeneration (11.66%)was obtained from Sakha 93. G168 also yielded high (11.00%) meannumber of plant regeneration .Albinoplants were obtained from 3 of the 5 cultivars. The stage of development of the microspores at the time of culture was affected on plant regeneration percent . Uninucleate microspores stage was identified as producing the highest percentages of green plants(26.66%) compared with the tetrad(15.37%) stage. It is also indicated that the Sakha 93 cultivars showed the highest percentage of green plants from the nucleate stage (35.55%) follow by G 168 . | ||||
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