An In-Vitro Evaluation of Alternative Disinfection Methods of Acrylic resin and Thermoplastic Resin Denture Base Materials | ||||
Al-Azhar Dental Journal for Girls | ||||
Article 4, Volume 5, Issue 3, July 2018, Page 243-255 PDF (445.61 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/adjg.2018.17186 | ||||
View on SCiNiTO | ||||
Authors | ||||
Dina Kholief1; Shereen Kabeel2 | ||||
1Assistant Professor of Removable Prosthodontics, Faculty of Dental Medicine for girls- Al-Azhar University. | ||||
2Lecturer of Removable Prosthodontics, Faculty of Dental Medicine for girls- Al-Azhar University. | ||||
Abstract | ||||
Objectives: This study was designed to evaluate the antimicrobial effect of alternative disinfection methods of heat cured acrylic resin and thermoplastic resin denture base materials and to investigate the porosity resulting from disinfection by these methods. Material and Methods: Disc specimens of heat cured acrylic resin and thermoplastic resin were fabricated and divided into four groups for each denture base material according to the disinfection method. The specimens were contaminated in vitro by standardized suspensions of Candida albicans (C. albicans).The following test agents were used: distilled water (as a control group), 5% sodium hypochlorite (NaOCl), 100% white vinegar and 650 W microwave (MW) energy. After the disinfection procedure, the number of viable microbial cells was counted in CFU/ml. Porosities of acrylic resin and thermoplastic resin specimens were evaluated with Scanning Electron Microscope (SEM) after one month of daily exposure to disinfection. Results: ANOVA test showed that, there was a statistical significant difference among the tested treatment agents against C. albicans in each denture base material(P≤ 0.05). Post Hoc test showed a statistical significant difference between the control samples and the other disinfected samples in the microbial count, however, there was no statistical significant difference among the tested methods of disinfection (P>0.05).Regarding porosity area percentage, there was a statistical significant difference among the tested methods of disinfection (P≤ 0.05), where, 100% white vinegar showed the highest porosities followed by NaOCl then the MW disinfection and the least is the control group. Regarding comparison between the two denture base materials, thermoplastic resin showed statistically significant lower microbial adherence as well as lower porosity area percentage than heat cured acrylic resin(P≤ 0.05). Conclusion: All the tested disinfection methods shown to be efficient against C. albicans. Thermoplastic resin demonstrated lower microbial adherence than heat cured acrylic resin and lower porosity area percentages. | ||||
Keywords | ||||
Candida albicans; Disinfection; denture base | ||||
References | ||||
1. Yildirim-Bicer AZ, Peker I, Akca G, Celik I. In vitro antifungal evaluation of seven different disinfectants on acrylic resins. Biomed Res Int 2014; 1-9. 2.
de Andrade IM1, Cruz PC, Silva-Lovato CH, de Souza RF, Souza-Gugelmin MC, ParanhosF 3.
Effect of chlorhexidine on denture biofilm accumulation 2012;21:2-6. 4. Singh S, Palaskar JN, Mittal S. Comparative evaluation of surface porosities in conventional heat polymerized acrylic resin cured by water bath and microwave energy with microwavable acrylic resin cured by microwave energy. Contemp Clin Dent 2013; 4: 147-51. 5.
Pires-de-Souza FC, Panzeri H, Vieira MA, Garcia L-FR, Consani S. Impact and fracture resistance of an experimental acrylic polymer with elastomer in different proportions. Mater Res 2009; 12:415-8. 6. Ardelean L, Bortun C, Podariu AC, Rusu LC. Some Alternatives for classic thermo-polymerisable acrylic dentures. Materiale Plastice 2012; 49:30-3. 7.
Gungor H, Gundogdu M, Duymus ZY. Investigation of the effect of different polishing techniques on the surface roughness of denture base and repair materials. J Prosthet Dent 2014; 112: 1271-7. 8.
Mohannad H. Al-Saadi. Effectiveness of chemical and microwave disinfection on denture biofilm fungi and the influence of disinfection on denture base adaptation. J Indian Prosthodont Soc 2014; 14: 24–30. 9. Cenci TP, Del Bel Cury AA, Crielaard W, Ten Cate JM. Development ofCandida-associated denture stomatitis: new insights. J Appl Oral Sci 2008; 16:86-94. 10. Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont 2011; 20:251-60. 11.
Oliveira H, Silva-Lovato C, De Souza R, Cruz, De Freitas- Pontes KM, Watanabe E. Effect of three methods for cleaning dentures on biofilms formed in vitro on acrylic resin. J Prosthodont 2009; 18: 427–31. 12. Kulak Y, Arikan A, Kazazoglu. Existence of Candida albicans and microorganisms in denture stomatitis patients. J Oral Rehabil1997; 24:788–90. 13.
Jagger D C, Harrison A. Denture cleansing - the best approach. Br Dent J 1995; 178:413-7. 14.
ParanhosHde F, Davi LR, Peracini A, Soares RB, Lovato CH, Souza RF. Comparison of physical and mechanical
properties of microwave-polymerized acrylic resin after disinfection in sodium hypochlorite solutions. Braz Dent J 2009; 20: 331-5. 15.
Nakahara T, Harada A, Yamada Y, Odashima Y, Nakamura K, Inagaki R, et al. Influence of a new denture cleaning technique based on photolysis of H2O2 the mechanical properties and color change of acrylic denture base resin. Dent Mater J 2013; 32:529-36. 16. Pinto T,Neves A,Leão M, Jorge. Vinegar as an antimicrobial agent for control of Candida spp. In complete denture wearers. J Appl Oral Sci 2008;16:385-90. 17. Peracini A, Regis RR, Souza RF, Pagnano VO, Silva CH, Paranhos HF. Alkaline peroxides versus sodium hypochlorite for removing denture biofilm: A crossover randomized trial. Braz Dent J 2016; 27:700-4. 18. Nascimento MS, Silva N, Catanozi MP, Silva KC. Effects of different disinfection treatment on the natural microbiota of lettuce. J Food Prot 2003; 66:1697-700. 19. Sesma N, Rocha AL, Laganá DC, Costa B, Morimoto S. Effectiveness of denture cleanser associated with microwave disinfection and brushing of complete dentures: In vivo study. Braz Dent J 2013; 24:357-61. 20. Silva M, Consani R, Sardi J, Mesquita M, Maceˆdo A, Takahashi J. Microwave irradiation as an alternative method for disinfection of denture base acrylic resins. Minerva Stomatol 2013; 62:23-9. 21. Altieri K, Sanita´ P, Machado A, Giampaolo E, Pavarina A,Vergani C. Effectiveness of two disinfectant solutions and microwave irradiation in disinfecting complete dentures contaminated with methicillin-resistant Staphylococcus aureus. J Am Dent Assoc 2012; 143:270–7. 22. Dovigo L, Pavarina A, Ribeiro D, de Oliveira J, VerganiC, Machado A. Microwave disinfection of complete dentures contaminated in vitro with selected bacteria. J Prosthodont 2009;18:611–7. 23. Silva M, Mima E, Colombo A, Sanita´ P, Jorge J, MassucatoE, Vergani C. Comparison of denture microwave disinfection and conventional antifungal therapy in the treatment of denture stomatitis: A randomized clinical study. Oral Surg Oral Med Oral Pathol Oral Radiol2012;114:469–79. 24. Sanita P, Machado A, Pavarina A, Massucato E, Colombo A,Vergani C. Microwave denture disinfection versus nystatinin treating patients with well-controlled type 2 diabetes and denture stomatitis: A randomized clinical trial. Int J Prosthodont 2012; 25:232–44. 25. Nirale RM, Thombre R, Kubasad G. Comparative evaluation of sodium hypochlorite and microwave disinfection on dimensional stability of denture bases. J Adv Prosthodont 2012; 4:24–9. 26. Senna P, Da Silva W, Faot F, Del Bel Cury A. Microwave disinfection: cumulative effect of different power levels on physical properties of denture base resins. J Prosthodont 2011; 20:606–12. 27. Basso M, Giampaolo E, Vergani C, Machado A, Pavarina A,Compagnoni M. Influence of microwave disinfection onthe linear dimensional stability of complete dentures: a clinical study. Int J Prosthodont 2010; 23:318–20. 28. Klironomos T, Katsimpali A, Polyzois G. The effect of microwave disinfection on denture base polymers, liners and teeth: A basic overview.ActaStomatol Croat 2015; 49(3): 242–53. 29.
Mohammed HS, Singh S, Hari PA, Amarnath GS, Kundapur V, Pasha N, et al. Evaluate the effect of commercially available denture cleansers on surface hardness and roughness of denture liners at various time intervals. Int J Biomed Sci 2016; 12: 130–42. 30. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26:577–83. 31. Al-Fouzan AF, Al-mejrad LA, Albarrag AM. Adherence of
Candida to complete denture surfaces in vitro: A comparison of conventional and CAD/CAM complete dentures. J AdvProsthodont2017; 9: 402–8. 32. Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent 1997;77:535-9. 33.
Ramage G, Martínez JP, López-Ribot JL. Candida biofilms on implanted biomaterials: a clinically significant problem. FEMS Yeast Res 2006;6:979–86. 34. Al-Khafagy M, Al-Musawi R, Al- Aboudy A. The effect of using modified flask on the porosity of processed heat
cure acrylic resin. IJSBAR 2013; 12: 189-7. 35. KasinaS , AjazT, AttiliS , Surapaneni
H , CherukuriM ,Srinath HP. To evaluate and compare the porosities in the acrylic mandibular denture bases processed by two different polymerization techniques, using two different brands of commercially available denture base resins - an in vitro study. J Int Oral Health 2014; 6: 72–7. 36.
Paranhos HFO, Peracini A, Pisani MX, Oliveira VC, Souza RF, Silva-Lovato CH. Color stability, surface roughness
and flexural strength of an acrylic resin submitted to simulated overnight immersion in denture cleansers. Braz Dent J 2013;24: 152-6. 37.
Köroğlu A, Şahin O, Dede DÖ, Deniz ŞT, Karacan Sever N, Özkan S
. Efficacy of denture cleaners on the surface roughness and Candida albicans adherence of sealant agent coupled denture base materials.Dent Mater J 2016; 35:810-6. 38. Ribeiro DG, Pavarina AC, Dovigo LN, Machado AL, Giampaolo ET, Vergani CE. Prevalence of Candida spp. associated with bacteria species on complete dentures. Gerodontology 2012;29:203-8. 39. Jackson S, Coulthwaite L, Loewy Z, Scallan A, Verran J
. Biofilm development by blastospores and hyphae of Candida albicans on abradeddenture acrylic resin surfaces.J Prosthet Dent 2014; 112:988-93. 40.
Işeri U, Uludamar A, Ozkan YK. Effectiveness of different cleaning agents on the adherence of Candida albicans to acrylic denture base resin. Gerodontology 2011; 28: 271-6. 41.
Sorgini DB, Silva-Lovato CH, Souza RF, Davi LR, Paranhos HFO. Abrasiveness of conventional and specific denture cleansing dentifrices. Braz Dent J 2012; 23: 154-9. 42. Elmorsy AE, Ibraheem EM, Ela AA, Fahmy A, Nassani MZ
. Do flexible acrylic resin lingual flanges improve retention of mandibular complete dentures? J Int SocPrev Community Dent 2015; 5:365-71. 43. Da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2008; 17: 627-33. 44.
Salles MM, Oliveira VC, Souza RF, Silva CHL, Paranhos HF. Antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning-in vitro evaluation. Braz Oral Res 2015; 29: 1-6. 45.
Fernandes FHCN, Orsi IA, Villabona CA. Effects of the peracetic acid and sodium hypochlorite on the color stability and surface roughness of the denture base acrylic resins polymerized by microwave and water bath methods. Gerodontology 2013; 30: 18-25. 46.
Harrison Z, Johnson A, Douglas WI. An in vitro study into the effect of a limited range of denture cleaners on surface roughness and removal of candiada albicans from conventional heat-cured acrylic resin denture base material. J Oral Rehabil 2004; 31: 460-7. 47.
Neppelenbroek KH, Pavarina AC, Palomari Spolidorio DM, Sgavioli Massucato EM, Spolidorio LC, Vergani CE. Effectiveness 0f microwave disinfection of complete dentures on the treatment of Candida- related denture stomatitis. Oral Rehabil 2008; 35:836-46. 48.
Campanha NH, Pavarina AC, Brunetti IL, Vergani CE, Machado AL, Spolidorio DMP. Candida albican sinactivation and cell membrane integrity damage by microwave irradiation. Mycoses 2007; 50:140-7. 49. Ribeiro DG, Pavarina AC, Dovigo LN, Spolidorio DM, GiampaoloET, Vergani CE. Denture disinfection by microwave irradiation: A randomized clinical study. J Dent 2009; 37:666-72. 50. Sesma N, Gil C, Kolikauskas WA, Silva RA, Pannuti CM. Temperature of denture base resin under different protocols of microwave irradiation. Braz Dent J 2011;22:388-91. 51. Komiyama EY, Back-BritoGN, Balducci I, Koga-Ito CY. Evaluation of alternative methods for the disinfection of tooth brushes. Braz Oral Res 2010; 24: 28-33. 52. Rudd R, SeniaE, McCleskey F, Adams ED. Sterilization of complete dentures with sodium hypochlorite. J Prosthet Dent 1984; 51:318-21. 53.
Banting DW, Hill SA. Microwave disinfection of dentures for the treatment of oral candidiasis. Spec Care Dentist 2001; 21:4-8. 54. Silva M, Vergani C, Giampaolo E, Neppelenbroek K, Spolidorio D, Machado A. Effectiveness of microwave irradiation onthe disinfection of complete dentures. Int J Prosthodont 2006; 19:288–93. 55. Sanita´ P, Vergani C, Giampaolo E, Pavarina A, Machado A. Growth of Candida species on complete dentures: effect of microwave disinfection. Mycoses 2009; 52:154–60. 56. Senna P, Sotto-Maior B, Silva W, Del Bel Cury A. Adding denture cleanser to microwave disinfection regimen to reduce the irradiation time and the exposure of dentures to high temperatures. Gerodontology 2013; 30:26–31. 57. Aslanimehr M, Rezvani S, Mahmoudi A, Moosavi N. Comparison of Candida Albicans adherence to conventional acrylic denture base materials and injection molding acrylic materials. J Dent 2017; 18: 61-4. 58.
Hayran Y, Sarikaya I, Aydin A, HazırTekin Y. Determination of the effective anti-candidal concentration of denture cleanser tablets on some denture base resins. J Appl Oral Sci 2018; 26: e2017007.
59.
Patil R, Sharma H, Gupta D. Comparative evaluation of biofilm development of Candida Albicans on abraded surfaces of heat cure PMMA and Flexi denture material: An in vitro study. IOSR-JDMS 2016; 15: 130-3. 60.
Sundari I, Andayani R, Harahap NF. Comparison of Candida albicans colony amount in heat-cured acrylic and thermoplastic nylon resin after immersion in Ulee Kareng coffee (Coffearobusta). Padjadjaran J Dent 2017;29:48-53. 61. Kodir K, Tanti I, Odang R W. Surface roughness of denture bases after immersion in fishcake vinegar solution. J Phys: Conf Ser 2017. 62.
Jang DE, Lee JY, Jang HS, Lee JJ, Son MK. Color stability, water sorption and cytotoxicity of thermoplastic acrylic resin for denture non metal clasp. J Adv Prosthodont 2015; 7:278-87. 63. Davi LR, Peracini A, Ribeiro Nde Q, Soares RB, da Silva CH
, Paranhos H de F, de Souza RF. Effect of the physical properties of acrylic resin of overnight immersion in sodium hypochlorite solution. Gerodontology 2010; 27:297-302. 64. Novais PM, Giampaolo ET, Vergani CE, Machado AL, Pavarina AC,
Jorge JH.The occurrence of porosity in relines acrylic resins. Effect of microwave disinfect-ion Gerodontology 2009; 26:65-71. 65.
Nandal S, Ghalaut P, Shekhwat H, Gulati MS. New era in denture base resins: A review. Dent J Adv Studies 2013; 1:136-43. | ||||
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