BIOACCUMULATION OF SOME HEAVY METALS AND HISTOPATHOLOGICAL, ULTRASTRUCTURE ALTERNATION IN KIDNEY, BRAIN AND LUNG OF MIGRATORY BIRDS AROUND MANZALA LAKE | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 21, Volume 58, Issue 134, July 2012, Page 1-17 PDF (10.82 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2012.173822 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| SANAA ABDO-EL-SHAMY | ||||
| Animal Health Research Institute, Alexandria Regional Laboratory, Pathology Department | ||||
| Abstract | ||||
| Migratory birds collected from September to March (2011-2012) from colonies on Lake of Manzala, in a variety of bird species, including mallards (Anas Platyrhynchos), pintails (Anas Acuta), teal (Anas Crecca) and coor (Atra Atra). Immediately, heavy metals were measured in kidneys, liver and brain. Results showed that heavy metals accumulations in birds tissue area under investigation were detected in following descending order: pb > Cd > Cu. Carcasses were in good post mortum condition, the birds was moderately emaciated. The spleen was mildly enlarged. The intestine got darker, swollen, the tissues of liver and kidney were damaged and fragile. The gross lesions mainly confined to the kidney which was dark or with haemorrhages and showed evidence of sever degeneration. The kidneys showed sever degenerative changes which was more prominent in the cortex than the medulla. The kidneys showed marked glomerular, tubular and interstitial pathological alternation. Many glomeruli were shrunked with invaginated diffuse hyalinized thickening of capillary endothelium. The tubules were dilated with accumulation of eosinophilic homogenous material in tubular lumina. Necrobiotic changes with nuclear pyknosis Kayorrhexis, karyolysis, cytolysis, interstial odema and fibrosis were observed in the kidney. Focal cellular necrosis was observed in the renal proximal tubule. There was focal haemorrhagic area in between the renal tubules, dilatation and congestion of blood capillaries and PAS positive deposits focally spread in the mesangial matrix. The tubule showed hyaline droplet degeneration and increased protein content by bromophenol blue stain. Semithin section of kidney stained by toulidine blue showed heavy metals. In the form of great number of dark granules in the apical sections of the tubular epithelial cell. Ultrastructural findings of glomeruli demonstrated proliferation of mesangial cells. Mitochondria in the cells showed some degree of swelling. Nuclear inclusion bodies and large residual bodies were present. The rough endoplasmic reticulum lost their characteristic arrangement. Lysosomes were numerous in some cells. Lysosomes in some cells contained dense bodies of varying sizes and large residual bodies were present. Marked clumping of chromatin with the aggregation of interchromatin material at the center of the nucleus with intranuclear inclusions bodies. Pericellular oedema, glioses or sattelosis in brain gray matter and demylenation or spongiosis in the white matter. Chromatolysis of the nucleus and cytoplasmic vaculation in the neuron of the cerebral cortex. Pulmonary congestion and the lung alveoli were markedly filled with erythrocytes. The intealveolar blood cappilaries. The lungs exhibited focal apoptosis at the apical portion of the cells and thickening of the interstitial connective tissue. | ||||
| Keywords | ||||
| Key words: Migatory birds; Heavt metals; Light & TEM | ||||
| References | ||||
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REFERENCES
Abbas, K.; Bagher, M.; Seyed, N.O. Ruhollah and Mohama, A. (2000): Biochemical and ultrastructure evidence for toxicity of lead through free radicals in rats brain. Humman and Experamintal Toxicology, 22(8): 417-425.
A.O.C (2006): Association of Official Agriculture Chemists Method 999. 11. The method available at internet: www.aoac .org/omarev/999_11.pdf.
Bancroft, J.D.; Stevens, A. and Turner, D.R. (1996): Theory and practice of histological techniques 4th ed. Churchill Living Stone, New York Edinburgh Madrid, San Francisco, Tokyo.
Barrett, R.T. and Krasnov, Y.V. (1996): Recent responses to changes in stocks of prey species by seabirds breeding in the southern barents sea. ICES Journal of Marine Science, 53: 713– 722.
Beyer, W.N.; Spann, J.W.; Sileol. and Fransom, J.C. (1988b): Lead soning in six captive avian species. Arch. Environ. Contam. Toxicol. 17, 121.
Bost, C.A. and Lemaho, Y. (1993): Seabirds as bio-indicators of changing marin ecosystem new perspectives. Acta Oecologica-International Journal 14: 463-470.
Bubel, A. (1976): Histological and electron microscopical observations on the effects of different salinities and heavy metal ions, on the gills of Jaera nordmanni (Rathke) (Crustacea, Isopoda). Cell Tiss. Res., 167: 65-95.
Burger, J. and Gochfeld, M. (1991): Lead, Mercury and Cadmium in feathers of tropical terns in puerto rico and australia. Archives of Environmental Contamination and Toxicology 21: 311–315.
Burce A. Fowler Carole A. Kimmel; James S. Woods; Ernest E. McConnell; Lester D. Granta (1980). Chronic low-level lead toxicity in the rat: III. An integrated assessment of long-term toxicity with special reference to the kidney. Toxicology and Applied Pharmacology Volume 56, Issue 1, Pages 59–77.
Buss, J.S. and Gibson, J.E. (1979): Lipid peroxidation and its role in toxicology. In: Hodgson E., Bend JR, Philpot RM (e d s) Reviews in biochemical toxcology. Elsevier, Amsterdam, pp. 125-149 .
Chang, L.W.; Wade, P.R. and Blson, M.J. (1980): Ulttrastructural changes in renal proximal tubules after tetraethyl lead intoxication. Envron. Res. 23: 208.
Cherel, Y. and Weimerskirch, H. (1995): Seabirds as indicators of marine resources black- albatrosses feeding on ommastrephid squids in Kerguelen aters. Marine Ecology Progress Series 129: 295–300.
Cheville, N.F. (1994): Ultrastructural Pathology. IowaStateUniversity Press, pp: 67-68. Ames.
Clausen, B. and Wolstrup, C. (1978): Copper load in mute swans (Cygnus olor) found in Denmark. Nord. Vet. Med. 30: 260-266.
Colle, A.; Grimaud, J.A.; Boucherat, M. and Manuel, Y. (1980): Lead poisoning in monkeys: functional and histopathological alterations of the kidneys. Toxicology, 18(2): 58-145.
Danielyan, A. (2010): The problem of pollution with heavy metals and possible risks related to that in watersheds with the developed metallurgical industry. In: BALWOIS 2010 Conference. Ohrid,Republic of Macedonia, pp. 1–9.
Egwurugwu, J.N.; Ufearo, C.S.; Abanobi, O.C.; Nwokocha, C.R.; Duribe, J.O.; Adeleye, G.S.; Ebunlomo, A.O.; Adetola, A.O. and Onwufuji, O. (2007): Effect of Ginger (Zingiber officinale) on cadmium toxicity. African Journal of Biotechnology, 6(18): 2078-2082.
Ezemonye Lawrence Ikechukwu and Enuneku Alex Ajeh (2011): Histopathological Alterations in The Liver And Lungs of Hoplobatrachus Occipitalis Exposed to Sub Lethal Concentrations of Cadmium Australian Journal of Basic and Applied Sciences, 5(11): 1062-1068.
Ferreira, AP. and Horta, MAP. (2010): Trace element residues in Water, Sediments, and Organs of Black-crowned Night Heron (Nycticorax nycticorax) from SepetibaBay, Rio de Janeiro, Brazil. Rev. Ambiente and Água, 5(1): 17- 28.
Fowler, B.A.; Kimmel, C.A.; Woods, J.S.; McConnel, E.E. and Grant, L.D. (1980): Chronic low- level lead toxicity in the rat: III. An integrated assessment of long-term toxicity with special reference to the kidney. Toxicol. Appl. Pharmacol. 56: 59-77.
Friberg, L.; Elinder, C-G.; Kjellstro,¨ MT. and Nordberg, GF. (1986): Cadmium and Health. A Toxicological and Epidemiological Appraisal, vol. II. Boca Raton, FL: CRC Press Inc,. (303 pp).
Furness and P. Monaghan "Eds." (1987): Seabird ecology. New York: Chapman & Hall.
Furness, R. and Camphuysen, K. (1997): Seabirds as monitors of the marine environment. ICES Journal of Marine Science 54: 726–737.
Geeth, G.; Raul, E. Martinez; Weiqun Xiao and Douglas M. Templeton (2005): Cadmium inhibits both intrinsic and extrinsic apoptotic pathways in renal mesangial cellsdoi: 10.1152/ajprenal.00067. Am J Physiol Renal Physiol 290: F1074-F1082.
George, S.G. (1982): Subcellular accumulation and detoxlca-tion of metals in aquatic anlmals. In: Vernberg, W.B., Calabrese, A., Thurberg, F.P., Vernberg, F.J. (eds.) Phy- siological mechanisms of marine pollutant toxlcity. Academic Press, New York, p. 3-52.
Goering, PL.; Waalkes, MP. and Klaassen, CD. (1995): Toxicology of cadmium. In: Handbook of Experimental Pharmacology, Vol. 115, Toxicology of Metals, Biochemical Aspects, edited by Goyer RA and Cherians MG. Berlin: Springer-Verlag, , p. 189–214.
Gochfeld, M. and Buger, J. (1987): Heavy metal concentration in the liver of three duck species, influence of species and sex. Environment pollution 45: 1-15 .
Goyer, R.A. (1971): Lead toxicity: A problem in environment pathology. Am. J. Path.; 64 (1): 167-181.
Goyer, R.A.; Leonard, D.L.; Moore, J.F.; Rhyne, B. and Kriyman, M.R. (1970): Lead dosage and the role of the intranuclear inclusion body. An experimental study. Arch Environ. Health.; 20: 705-711.
Gray, J. (2002): Biomagnification in marine systems: the perspective of an ecologist. Marine Pollution Bulletin 45: 46–52.
Hamada, T.; Tanimoto, A.; Iwai, S.; Fujiwara, H. and Sasaguri, Y. (1994): Cytopathological changes induced by cadmium exposure in canine proximal tubular cells: a cytochemical and ultrastructural study. Nephron 68: 104–111.
Honda, K.; Marcovecchio, J.E.; Kan, Tatsukawa, S.R. and Ogi, H. (1990): Metal concentration in pelagic seabirds from the north Pacific ocean. Arc. Environm. Contam. Toxicol. 19: 704-711.
Jacobs, R.M.; Fox , M.R.S. and Ldridge, M.H.A. (1969): Changes in plasma proteins associated with the anemia produced by dietary cadmium in Japanese quail. The Journal of Nutrition 99: 119-128.
Jarrar, B.M. (1999): Histological, histochemical and Saudi Journal of Biological Sciences Vol. 15, No (2).
Karmakar, N.; Saxena, R. and Anand, S. (1986): Histopathological changes induced in rat tissues by oral intake of lead acetate. Environ. Res. 41: 23-28.
Kim, E.Y.; Goto, Tanabe, S.; Tanaka, H. and Tatsukawa, R. (1998): Distrubution of 14 trace element in tissues and organs of oaceanic sea birds ,Archives of Enviroment contamination and toxicology 35: 638-645.
King, DW.; Fenoglia, C.M. and Leckowich, J.H. (1983): General pathology princeples and dynamics Leoand Febger.
Lee, D.P.; Honda, K.; Tatsukawa, R. and Won, P.O. (1989). Distribution and residue level of mercury, cadmium and lead in Korean birds. Bull. Environ. Contain. Toxicol.43: 550-555.
Leonz, C.; Fossi, C. and Focardi, (1986): Heavy metals and selenium variation in a Migratory bird wintering in a Mercory-polluted lagoon. Sci. Total Environ., 57: 121–127.
Lin, JL.; Yeh, KH.; Tseng, HC.; Chen, WY.; Lai, HH. and Lin, YC. (1993): Urinary N-acetyl- glucosaminidase excretion and environmental lead exposure. Green Cross Health Service Association Study Group. Am. J. Nephrol. 13: 442- 447.
Liu, J.; Habeebu, SS.; Liu, Y. and Klaassen, CD. (1998): Acute CdMT is not a good model to study chronic Cd nephropathy: comparison of chronic CdCl and CdMT exposure with acute CdMT 2 injection in rats.T oxicol Appl Pharmacol;153: 48–58.
Llacuna, S.; Gorriai, T.; Ikemoto, T.; Hokura, A.; Terada, Y.; Kunito, T.; Tanabe, S. and Nakai, I. (2004): Chemical forms of mercury and cadmium accumulated in marine mammals and seabirds as determined by XAFS analysis. Environmental Science and Technology 38(24): 6468-6474 .
Lock, J.W.; Thompson, D.R.; Furness, R.W. and Bartle, J.A. (1992): Metal concentrations in seabirds of the New Zealand region. Environm. Pollut. 75: 289-300.
Mansour, I.; Naief, M.; Al-Otaibi Saud, A.; Salah A Ibrahim and Bashir M. Jarrar (2008): Histological and histochemical alternation induced by lead in the kidney of the quail Coturnix Coturnix soudi Journal of biological science15 (2): 307–313.
Monteiro, LR. and Furness, RW. (1995): Seabirds as monitors of mercury in the marine environment. Water, Air and Soil Pollution 80: 851–870.
Moore, M. N. (1985): Cellular responses to pollutants. Mar. Pollut. Bull. 16: 134-139.
Moore, M.N.; Pipe, R.K. and Farrar, S.V. (1982): Lysosomal and microsomal responses to environmental factors in Littorina littorea from Sullom Voe. Mar Pollut. Bull. 13: 340-345.
Myers, M.S.; Johnson, L.L.; Olson, O.P.; Stehr, C.M.; Horness, B.H.; Collierand, T.K. and McCain, B.B. (1998): Toxicopathic hepatic lesions as biomarkers of chemical contaminant exposure and effects in marine bottom fish species from the Northeast and pacific coasts, USA. Mar. Pollut. Bull., 37: 92-113.
Nicholson, J.K. and Osborn, D. (1983): Kidney lesions in pelagic seabirds with high tissue levels of cadmium anAdd mercury Journal of Zoology Volume 200, Issue 1, pages 99–118.
Norheim, G. (1987): Levels and interactions of heavy metals in sea birds from Svalbard and the Antarctic . Environ. Pollut. 47: 83-94.
Pietrapiana, D.; Modena, M.; Guidetti, P.; Falugi, C. and Vacchi, M. (2002): Evaluating the genotoxic damage and hepatic tissue alterations in demersal fish species: A case study in the LigurianSea (NW- Mediterranean). Mar. Pollut. Bull., 44: 238-243.
Roberts, J.R. (1999): Metal toxicity in children. Training manual on pediatric environmental health: putting it into practice. Emeryvile CA. Childrens’ environmental health network.
Seebaugh, DR.; Goto, D. and Wallace, WG. (2005): Bioenhancement of cadmium transfer along a multi-level food chain. Marine Environ. Res., 59(5): 473–491.
Scott, R.; Aughey, E. and Sinclair, J. (1977): Histological and ultrastructural changes in rat kidney following cadmium injection.Ur ol Res; 5: 15–20.
Sonne, C.; Hansen, A.; Dietza, R.; Leifssonb, P.S.; Hyldstrup, L. and Riget, C. (2002): Cadmiumtoxicity to ringed seals (Phoca hispida): anepidemiological study of possible cadmium- induced nephropathy and osteodystrophy in ringed seals (Phoca hispida) from Qaanaaq in North west Greenland The Science of the Total Environment 295: (167–181).
Soudani, N.; Sefi, M.; Ben Amara, I.; Boudawara, T. and Zeghal, N. (2010): Protectivee effects of selenium (se)induced nephrotoxicity in adult rats Ecotoxicol .Environ. saf. 73: 671-678.
Struzynska, L. and Dabrowska-boutu, B. rafalowaska (1997): Acute lead toxicity and energy metabolism in rat brain synaptosomes. Acta neurobiol, Exp, 57: 275-81.
Squibb, KS.; Ridlington, JW.; Carmichael, NG. and Fowler, BA. (1979): Early cellular effects of circulating cadmium–thionein on kidney C.proximal tubules.Envir on Health Perspect;28: 287–296.
Sujatha, K.; Srilatha, C.H.; Anjaneyului, Y. and Marvathi, P.A. (2011): Lead acetate induced nephrotoxicity in wistar albino rats. A pathological, immunohistochemical and ultrastructural studies
Tanimoto, A.; Hamada, T.; Higashi, K. and Sasaguri, Y. (1999): Distribution of cadmium and metallothionein in CdCl2 –exposed rat kidney: Relationship with apoptosis and regeneration. Pathology International, 49: 125–132.
Thophon, S.K.; Kruatrachue, M.; Upathan, E.S.; Pokthitiyook, P.; Sahaphong, S. and Jaritkhuan, S. (2003): Histopathological alterations of white sea bass, Lates calcarifer, in acute and subchronic cadmium exposure. Environ. Pollut. 121: 307–320.
Turner, J.C.; Solly, S.R.B.; MoI-Krijnen, J.C.M. and Shanks, V. (1978): Organochlorine, fluorine and heavy-metal levels in some birds from New Zealand estuaries. NZ J. Sci. 21: 99-1O2.
Walther, U.I.; Czermak, A.; Mückter, S.C. and Fichtl, B. (2003): Decreased GSSG reductase activity enhances cellular zinc toxicity in the three human lung cell lines.Archives of Toxicology, 77: 131-137.
WHO. IPCS Environmental Health Criteria 134: Cadmium.Geneva: WHO, (1992): p. 1 –209.
Yasuda, M.; Miwa, A. and Kitagawa, M. (1995): Morphometric studies of renal lesions in itai itai disease: chronic cadmium nephropathy. Nephr on; 69:14–19.
Zheng, W. (2001): Neurotoxicology of the brain barrier system; New implantation clinical toxicology, 39(7): 711-19 .
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