HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM
Mima, A., Ghriwati, A., Khanji, K. (2021). HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM. EKB Journal Management System, 44(1), 111-118. doi: 10.21608/bfsa.2021.174136
Abdulkader Mima; Abdel Galil Ghriwati; Khalid Khanji. "HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM". EKB Journal Management System, 44, 1, 2021, 111-118. doi: 10.21608/bfsa.2021.174136
Mima, A., Ghriwati, A., Khanji, K. (2021). 'HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM', EKB Journal Management System, 44(1), pp. 111-118. doi: 10.21608/bfsa.2021.174136
Mima, A., Ghriwati, A., Khanji, K. HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM. EKB Journal Management System, 2021; 44(1): 111-118. doi: 10.21608/bfsa.2021.174136

HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM

Bulletin of Pharmaceutical Sciences Assiut University
Article 11, Volume 44, Issue 1, June 2021, Page 111-118  XML PDF (517.53 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2021.174136
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Authors
Abdulkader Mimaorcid 1; Abdel Galil Ghriwati1; Khalid Khanji2
1Department of Biotechnology Engineering, Faculty of Technology, Aleppo University, Aleppo, Syria
2Department of Pediatrics, Faculty of Medicine, Aleppo University, Aleppo, Syria
Abstract
Fetal bovine serum (FBS) is frequently used as a growth factor and as a source of proteins in culture media, but it may contain pathogens.In this study, we aimed to evaluate the possibility of using human platelet lysate (HPL) as a substitute for FBSin bone marrow karyotyping medium. The study included 30 samples of bone marrow aspiration from patients attending the Aleppo University Hospital. Our results showed that the concentration of IGF-1 and GH in HPL was (117 ng/ml) and (2.6 mIU/L) respectively.We also determined the mitotic index (MI), and the results showed that MI values were higher when using the medium supplied with HPLcompared to medium supplied with FBS.Statistical study also showed that there were significant differences (p= 0.001) in MI values when comparing the two media. Our results suggest that HPLcan be used as a substitute for FBSin bone marrow karyotyping medium.
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