IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT
Mousa, R., El-Kady, M., Hussein, A., Ahmed, A., El-Gendy, A. (2021). IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT. EKB Journal Management System, 44(1), 161-172. doi: 10.21608/bfsa.2021.174141
Rehab Mousa; Magdy F. El-Kady; Asmaa A. A. Hussein; Amr ُE. Ahmed; Ahmed Osama El-Gendy. "IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT". EKB Journal Management System, 44, 1, 2021, 161-172. doi: 10.21608/bfsa.2021.174141
Mousa, R., El-Kady, M., Hussein, A., Ahmed, A., El-Gendy, A. (2021). 'IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT', EKB Journal Management System, 44(1), pp. 161-172. doi: 10.21608/bfsa.2021.174141
Mousa, R., El-Kady, M., Hussein, A., Ahmed, A., El-Gendy, A. IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT. EKB Journal Management System, 2021; 44(1): 161-172. doi: 10.21608/bfsa.2021.174141

IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT

Bulletin of Pharmaceutical Sciences Assiut University
Article 16, Volume 44, Issue 1, June 2021, Page 161-172  XML PDF (695.49 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2021.174141
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Authors
Rehab Mousa1; Magdy F. El-Kady2; Asmaa A. A. Hussein3; Amr ُE. Ahmed4; Ahmed Osama El-Gendy5
1Pharmacist at Al-Azher University Hospital, Assiut, Egypt and Deparmtent of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt
2Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
3Department of Zoonoses, Faculty of Veterinary Medicine, and Dean of Molecular Biology Researches & Studies Institute, Assiut University, Assiut, Egypt
4Deparmtent of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt
5Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt
Abstract
Purpose: Study aimed to determine the frequency of nosocomial infections caused by P. aeruginosa and its distribution among different intensive care units (ICUs), to determine the antibiotic resistance pattern, and to determine the prevalence of Metallo-b-lactamase (MbL) among isolates by phenotypic methods. Methods: A total of 74 P. aeruginosa isolates were identified from different clinical specimens in AL-Azher University Hospital. The antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method and phenotypic screening for MbLs was performed using Combined Disk Test (CDT) and double-disk synergy test (DDST). Results: The frequency of P. aeruginosa isolates from clinical specimens was 18% (74/412). The highest sensitivity was to imipenem 38 isolates (51.3%). The antibiotic sensitivity was in descending manner to meropenem (48.6%) > levofloxacin (28.4%) > ciprofloxacin and ceftazidime (21.6%), while the highest resistance rates were to carbenicillin 70 isolates (94.6%) then gentamicin (70.3%). Out of 74 P. aeruginosa isolates, 51/74 (69%) strains were multidrug resistant (MDR), based on the CDT results 26/74 (35.1%) isolates and by DDST 23/74 (31.1%) isolates were confirmed to be MbLs producers. Conclusion: There is a growing risk for isolation of MDR P. aeruginosa among the MBL-producer isolates suggests the need for continuous assessment of antimicrobial susceptibility and surveillance of antibiotic prescription. In addition, infection control measures are needed to prevent further dissemination of these organisms.
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