VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT
Mandour, S., Abdelmohsen, A., Medhat, M., Abdeltawab, D., Elfarash, A., Ahmed, A., Galal, A., El-Mokhtar, M. (2021). VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT. EKB Journal Management System, 44(1), 213-224. doi: 10.21608/bfsa.2021.174147
Sahar A. Mandour; Ahmed Shawkat Abdelmohsen; Mohammed A. Medhat; Doaa Abdeltawab; Ameer Elfarash; Abdulrahman K. Ahmed; Ahmed M. Galal; Mohamed A. El-Mokhtar. "VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT". EKB Journal Management System, 44, 1, 2021, 213-224. doi: 10.21608/bfsa.2021.174147
Mandour, S., Abdelmohsen, A., Medhat, M., Abdeltawab, D., Elfarash, A., Ahmed, A., Galal, A., El-Mokhtar, M. (2021). 'VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT', EKB Journal Management System, 44(1), pp. 213-224. doi: 10.21608/bfsa.2021.174147
Mandour, S., Abdelmohsen, A., Medhat, M., Abdeltawab, D., Elfarash, A., Ahmed, A., Galal, A., El-Mokhtar, M. VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT. EKB Journal Management System, 2021; 44(1): 213-224. doi: 10.21608/bfsa.2021.174147

VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT

Bulletin of Pharmaceutical Sciences Assiut University
Article 21, Volume 44, Issue 1, June 2021, Page 213-224  XML PDF (497.12 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2021.174147
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Authors
Sahar A. Mandour1; Ahmed Shawkat Abdelmohsen2; Mohammed A. Medhat2; Doaa Abdeltawab2; Ameer Elfarash3; Abdulrahman K. Ahmedorcid 4; Ahmed M. Galal5; Mohamed A. El-Mokhtar6
1Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 61111, Egypt
2Department of Tropical Medicine and Gastroenterology, Faculty of Medicine Assiut University, Assiut, Egypt
3Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, Egypt
4Medical Student, Faculty of Medicine, Assiut University, Assiut, Egypt
5Postgraduate Student, Department of Surgical Oncology, Sohag Cancer Center, Sohag, Egypt
6Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
Background: Helicobacter pylori (H. pylori) is a common cause of gastric ulcers and is a risk factor for gastric carcinoma. Little data are available about the characters of H. pylori causing infections in patients from Upper Egypt. Therefore, the aim of this study was to investigate the frequency of H. pylori infection in Egyptian patients undergoing endoscopy and complaining of persistent upper gastrointestinal symptoms directly in biopsy specimens using PCR technique and to study the associated virulence-related genes in H. pylori positive samples. Materials and Methods: One hundred and twenty gastric biopsy specimens were collected from Egyptian patients admitted to Assiut University Hospital and complaining of persistent upper gastrointestinal symptoms. H. pylori DNA was extracted for molecular identification by PCR. Positive samples were further analyzed to investigate the presence of different virulence-related genes and gene combinations. Results: H. pylori was detected in 92/120 (76.6%) of collected biopsies. All strains carried the vacA s1 subtype. The prevalence of the virulence genes cagA, cagE, iceA1, iceA2 and oipA were 79 (84.8%), 41(44.5%), 69 (75%), 44 (47.8%), and 55(59.7%) of the H. pylori positive samples, respectively. Genetic analysis showed that H. pylori were grouped into 29 different genotype combinations. The s1/m1/i1/d1/c1/cagA/iceA1/oipA genotype was the most predominant. Conclusion: Our results show a high frequency of H. pylori infections among Egyptian patients with gastrointestinal symptoms. The s1/m1/i1 vacA hybrid is the most prevalent subtype. The distribution of the different virulence-related genes shows alarming rates of cagA, cagE, iceA1, iceA2 and oipA. Careful monitory of H. pylori infections and investigation of their genetic characters should be carried out to control the spread of the virulent strains.
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