MESENCHYMAL STEM CELLS RIF1, TCL1A, AND TERT MARKERS AS INDICATORS OF AGE ESTIMATION IN RATS | ||||
The Egyptian Journal of Forensic Sciences and Applied Toxicology | ||||
Article 4, Volume 21, Issue 2, June 2021, Page 33-45 PDF (574.52 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejfsat.2020.27855.1140 | ||||
View on SCiNiTO | ||||
Authors | ||||
Fatma Mohamed Hassan 1; Hoda Ahmed Mohamed Basyoni2; Dina Sabry Abdelfattah3; Heba Abdo Abdel Razik2 | ||||
1Forensic medicine and clinical Toxicology department, faculty of medicine, Cairo university | ||||
2Forensic medicine and Clinical toxicology, Faculty of medicine, Cairo University, Egypt. | ||||
3Medical Biochemistry and Molecular Biology, Faculty of medicine, Cairo University, Egypt | ||||
Abstract | ||||
Background: Research on stem cells has become one of the most promising and advanced scientific topics. Nevertheless, there is a lack of this type of research in the field of forensic diagnostics. Age estimation is one of the main worked-on issues in forensic practice and research. Still, there is a need to explore new complementary reliable methods. This study aimed to detect the possibility of age estimation via age-associated alteration of RIF-1 (Replication timing regulatory factor 1), TCL1A (T-cell leukemia/lymphoma-1A), and TERT (Telomerase reverse transcriptase) gene expression in rats bone marrow stem cells. Methods: Isolation of bone marrow mesenchymal stem cells from rats were undertaken at different ages; 2, 4, 6, 8, 10, and 12 weeks old. RNA extraction was performed then total RNA was reverse transcribed to cDNA to detect the quantitative expression of RIF-1, TLC1A, and TERT by using the qRT-PCR then immunoblotting. Results: There were statistically significant increases in RIF1 and TCL1A mRNA and protein expression and a decrease in TERT mRNA and protein expression with age. Conclusion: Our results showed that the mRNA and protein expression of RIF-1, TCL1A, and TERT in bone marrow cells of rats could be useful indicators for age estimation in forensic practice. | ||||
Keywords | ||||
age estimation; mRNA; RIF-1; TCL1A; TERT; mesenchymal stem cells | ||||
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