Identification of chromosomes and aberrations after exposure to pesticide in bovine lymphocytes | ||||
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control | ||||
Article 2, Volume 2, Issue 2, December 2010, Page 5-9 PDF (267.54 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsf.2010.17440 | ||||
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Authors | ||||
Galdíková Martina; Šiviková Katarína; Holečková Beáta; Dianovský Ján; Mesarč Martin | ||||
University of Veterinary Medicine and Pharmacy, Department of Veterinary Genetics, Komenského 73, 04181 Košice, Slovak Republic, Europe | ||||
Abstract | ||||
Tebuconazole belongs to the group of triazole fungicides. As a systemic foliar fungicide it was widely used in agriculture for crops protection, such as barley, wheat, peanuts and orchard fruits. Associations between exposure to pesticides and health outcomes in humans, including different kinds of cancer were reported by several authors. Effects in immune, hematological, nervous, endocrine and reproductive systems have also been described. Data about genotoxic effects of pesticides are rarely reported. We are interested in the detection of structural and numerical aberrations in bovine peripheral lymphocytes using fluorescence in situ hybridization (FISH) in vitro. Three whole chromosome painting probes (wcps), BTA1 for the bovine chromosome 1 (red colour), BTA 5 for chromosome 5 (green colour) and BTA 7 for chromosome 7 (red colour), as well, were used in experiments. Aneuploidy and unstable structural aberrations were detected more precisely than those seen in conventional chromosome aberrations assays, but only on the marked chromosomes could be analysed. | ||||
Keywords | ||||
tebuconazole-based fungicide; bovine peripheral lymphocytes; whole chromosome painting probes; fluorescence in situ hybridization | ||||
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