Anticancer activity of isolated compounds from twigs of Koelreuteria elegans cultivated in Egypt

Abo-Elghiet, Fatma; Ibrahim, Magda; Sleem, Amany A

doi:10.21608/aijpms.2021.53897.1022

Anticancer activity of isolated compounds from twigs of Koelreuteria elegans cultivated in Egypt

Azhar International Journal of Pharmaceutical and Medical Sciences

Article 3, Volume 1, Issue 2, June 2021, Page 23-29 PDF (294.46 K)

Document Type: Original research articles

DOI: 10.21608/aijpms.2021.53897.1022

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Authors

Fatma Abo-Elghiet ¹; Magda Ibrahim¹; Amany A Sleem²

¹Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

²Department of Pharmacology, National Research Centre, Giza, Egypt.

Abstract

Phytochemical constituents have been participated with essential role in the discovery of several clinically effective anticancer agents. The goal of this study was to isolate phytoconstituents from Koelreuteria elegans twigs (Sapindacea family) and evaluate their anticancer activities against human lung, colon and breast carcinoma cell lines. The aqueous methanol extract of plant's twigs was fractionated with different solvents and the preliminary cytotoxicity of fractions was determined using brine shrimp lethality assay. Nine compounds from K. elegans twigs butanol fraction were isolated for the first time; gallic acid (1), methyl gallate (2), 6-O- [Galloyl 4- methyl ether]-(α/β)-D-glucopyranose (3), 3, 5-di-O-galloylquinic acid butyl ester (4), 3,4,5-tri-O-galloylquinic acid butyl ester (5), 1,3,4,5-tetra-O-galloylquinic acid butyl ester (6), two isomers of 3-O-galloyl quinic acid butyl ester and 4-O-galloyl quinic acid butyl ester (7), austrobailignan 1 (8) and β-sitosterol (9). The structures of compounds were elucidated using different spectroscopic techniques. Anticancer activities of butanol fraction, methyl gallate and austrobailignan 1were estimated in-vitro by cell viability assay. The results showed significant anticancer activity of both methyl gallate and austrobailignan 1 against breast carcinoma cell lines and less cytotoxicity against colon carcinoma cell lines while almost no activity against lung carcinoma cell lines.

Keywords

Koelreuteria elegans; Gallic Acid; Methyl Gallate; Austrobailignan 1; Anticancer; Brine Shrimp; Cell Lines

Supplementary Files

Supplementary data 22.pdf

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	Anticancer activity of isolated compounds from twigs of Koelreuteria elegans cultivated in Egypt
Azhar International Journal of Pharmaceutical and Medical Sciences
Article 3, Volume 1, Issue 2, June 2021, Page 23-29 PDF (294.46 K)
Document Type: Original research articles
DOI: 10.21608/aijpms.2021.53897.1022
View on SCiNiTO
Authors
Fatma Abo-Elghiet ¹; Magda Ibrahim¹; Amany A Sleem²
¹Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt
²Department of Pharmacology, National Research Centre, Giza, Egypt.
Abstract
Phytochemical constituents have been participated with essential role in the discovery of several clinically effective anticancer agents. The goal of this study was to isolate phytoconstituents from Koelreuteria elegans twigs (Sapindacea family) and evaluate their anticancer activities against human lung, colon and breast carcinoma cell lines. The aqueous methanol extract of plant's twigs was fractionated with different solvents and the preliminary cytotoxicity of fractions was determined using brine shrimp lethality assay. Nine compounds from K. elegans twigs butanol fraction were isolated for the first time; gallic acid (1), methyl gallate (2), 6-O- [Galloyl 4- methyl ether]-(α/β)-D-glucopyranose (3), 3, 5-di-O-galloylquinic acid butyl ester (4), 3,4,5-tri-O-galloylquinic acid butyl ester (5), 1,3,4,5-tetra-O-galloylquinic acid butyl ester (6), two isomers of 3-O-galloyl quinic acid butyl ester and 4-O-galloyl quinic acid butyl ester (7), austrobailignan 1 (8) and β-sitosterol (9). The structures of compounds were elucidated using different spectroscopic techniques. Anticancer activities of butanol fraction, methyl gallate and austrobailignan 1were estimated in-vitro by cell viability assay. The results showed significant anticancer activity of both methyl gallate and austrobailignan 1 against breast carcinoma cell lines and less cytotoxicity against colon carcinoma cell lines while almost no activity against lung carcinoma cell lines.
Keywords
Koelreuteria elegans; Gallic Acid; Methyl Gallate; Austrobailignan 1; Anticancer; Brine Shrimp; Cell Lines
Supplementary Files Supplementary data 22.pdf

Statistics Article View: 325 PDF Download: 290