Antioxidant activity of Malpighia glabra L., leaves extract

Fekry, Amany; Elsabbagh, walaa; Abu Bakr, Marwa; El-Ghazaly, Mona; Mohamed, Abd El-Salam

doi:10.21608/aijpms.2021.59935.1042

Antioxidant activity of Malpighia glabra L., leaves extract

Azhar International Journal of Pharmaceutical and Medical Sciences

Article 10, Volume 1, Issue 2, June 2021, Page 88-93 PDF (307.88 K)

Document Type: Original research articles

DOI: 10.21608/aijpms.2021.59935.1042

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Authors

Amany Fekry ¹; walaa Elsabbagh¹; Marwa Abu Bakr ²; Mona El-Ghazaly¹; Abd El-Salam Mohamed³

¹Department of Drug Radiation Research, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

²Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

³Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt

Abstract

Malpighia glabra. L (Acerola) fruits have wide global medicinal uses due to rich antioxidant contents. Little studies were carried on other parts of plant. Malpighia glabra. L leaves (1.5 kg) was subjected to soxhlet exhaustive extraction with methanol and concentrated under vacuum. The dried extract was defatted with petroleum-ether then fractionated with ethyl acetate and n-butanol 3 successive times respectively. The ethyl acetate fraction (9 gm) was subjected to series of silica gel and Sephadex LH-20 column chromatography. Phytochemical investigation of the Ethyl acetate fraction of Malpighia glabra. L leaves in this study resulted in separation of four pure compounds (Caffeic acid, Chlorogenic acid, Quercetin & Kaempferol). The structures of the separated compounds were assigned based on NMR spectral data and mass spectrometer analysis. The methanolic extract showed high values of total phenolics (96.36 mg GAE/g), flavonoids (30.71 mg QE/g) and higher antioxidant activity towards DPPH radical with IC50 = 49.8(mg/ml).

Keywords

Malpighia glabra L. leaves; DPPH; Caffeic Acid; Chlorogenic Acid; Quercetin; Kaempferol

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	Antioxidant activity of Malpighia glabra L., leaves extract
Azhar International Journal of Pharmaceutical and Medical Sciences
Article 10, Volume 1, Issue 2, June 2021, Page 88-93 PDF (307.88 K)
Document Type: Original research articles
DOI: 10.21608/aijpms.2021.59935.1042
View on SCiNiTO
Authors
Amany Fekry ¹; walaa Elsabbagh¹; Marwa Abu Bakr ²; Mona El-Ghazaly¹; Abd El-Salam Mohamed³
¹Department of Drug Radiation Research, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt
²Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt
³Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt
Abstract
Malpighia glabra. L (Acerola) fruits have wide global medicinal uses due to rich antioxidant contents. Little studies were carried on other parts of plant. Malpighia glabra. L leaves (1.5 kg) was subjected to soxhlet exhaustive extraction with methanol and concentrated under vacuum. The dried extract was defatted with petroleum-ether then fractionated with ethyl acetate and n-butanol 3 successive times respectively. The ethyl acetate fraction (9 gm) was subjected to series of silica gel and Sephadex LH-20 column chromatography. Phytochemical investigation of the Ethyl acetate fraction of Malpighia glabra. L leaves in this study resulted in separation of four pure compounds (Caffeic acid, Chlorogenic acid, Quercetin & Kaempferol). The structures of the separated compounds were assigned based on NMR spectral data and mass spectrometer analysis. The methanolic extract showed high values of total phenolics (96.36 mg GAE/g), flavonoids (30.71 mg QE/g) and higher antioxidant activity towards DPPH radical with IC50 = 49.8(mg/ml).
Keywords
Malpighia glabra L. leaves; DPPH; Caffeic Acid; Chlorogenic Acid; Quercetin; Kaempferol


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