Ameliorative Effects of induced pluripotent stem cells on experimentally induced ovarian ablation in adult female rats | ||||
Benha Veterinary Medical Journal | ||||
Article 10, Volume 40, Issue 1, March 2021, Page 47-51 PDF (1.08 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bvmj.2021.66537.1353 | ||||
View on SCiNiTO | ||||
Authors | ||||
Asmaa Abdel monsef Sultan 1; Nesreen I. Salem2; Ibtisam M. Azam3; Ayman S. Farid4 | ||||
1Clinical pathology department,faculty of veterinary medicine,benha university | ||||
2Histology and Cell Biology, Faculty of Medicine, Benha University. | ||||
3Pathology Department, Animal Health Research Institute | ||||
4Clinical Pathology Department, Faculty of Veterinary Medicine, Benha University. | ||||
Abstract | ||||
The current study designed to evaluate the role of pluripotent stem cells (iPSc) on experimentally-induced ovarian ablation (OA) by evaluating of hematological parameters, FSH and E2 changes, molecular and histopathological examination. 50 female rats were divided into five groups with 10 in each group. Group (1): control group. Group (2): rats injected with Doxorubicin (DOX) (3 mg/kg) and Cyclophosphamide (CYP) (50mg/kg) dissolved in sterile physiological saline injected I/P once a week for 5 weeks. Group (3): 10 rats undergo chemoablasion then injected with 5 IU of pregnant mare serum gonadotropin (PMSG) single s/c injection. Group (4): 10 rats undergo chemoablasion, then iPSc injected i/v single injection for 2 months. Group (5): 10 rats undergo chemoablasion then iPSc injected i/v combined with single s/c injection of 5 IU PMSG. Results revealed that rats injected with Dox and CYP showed asignificant decrease in RBCs, Hb and PCV, WBCs, lymphocytes, neutrophil, eosinophil, E2 level and Oct 4 while there was significant increase in MCV, MCH, monocytes and FSH level compared with control (-ve) group. While, rats treated with PMSG or IPSc or PMSG and IPSc revealed significant increase in RBCs count as well as MCHC, E2 level and Oct 4 with non significant changes in Hb and PCV, WBCs, lymphocytes, eosinophil with asignificant increase of neutrophil IPSc or PMSG and IPSc groups and asignificant decrease in monocytes compared with OA group and control (-ve) group. | ||||
Keywords | ||||
ovarian ablation; chemotherapy; Doxorubicin; Cyclophosphamide and Induced Pluripotent Stem Cells | ||||
Statistics Article View: 160 PDF Download: 169 |
||||