Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma
elashrey, M., Desoky, A., Zyan, K. (2021). Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma. EKB Journal Management System, 40(1), 89-93. doi: 10.21608/bvmj.2021.61404.1336
mahmoud elashrey; Afaf Desoky; Kamel Zyan. "Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma". EKB Journal Management System, 40, 1, 2021, 89-93. doi: 10.21608/bvmj.2021.61404.1336
elashrey, M., Desoky, A., Zyan, K. (2021). 'Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma', EKB Journal Management System, 40(1), pp. 89-93. doi: 10.21608/bvmj.2021.61404.1336
elashrey, M., Desoky, A., Zyan, K. Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma. EKB Journal Management System, 2021; 40(1): 89-93. doi: 10.21608/bvmj.2021.61404.1336

Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma

Benha Veterinary Medical Journal
Article 18, Volume 40, Issue 1, March 2021, Page 89-93  XML PDF (909.9 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2021.61404.1336
View on SCiNiTO View on SCiNiTO
Authors
mahmoud elashrey email 1; Afaf Desoky1; Kamel Zyan2
1Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.
2Department of rabbits and birds diseases, Faculty of Veterinary Medicine, Benha University, Egypt
Abstract
Doxorubicin is a broad spectrum antitumor antibiotic. Newcastle disease virus (NDV ) is one such virus with an inherent oncolytic property. This study was done to investigate the antitumor effects of Doxorubicin and NDV on Diethylnitrosamine (DEN) induced Hepatocellular carcinoma in rats. Seventy five male albino rats were divided into five groups. Group (1): rats administered distilled water only. Group (2) : rats received diethylnitrosoamine (200 mg/kg b.wt/i.p), three times at an interval of 15 day at experimental weeks 2, 4 and 6.Group (3) : rats received DEN then treated with Doxorubicin at experimental week 10 at a dose level (2 mg/kg b.wt/i.p). Group(4): rats received DEN then treated with Hitchner B1 at experimental week 10 at a dose levels (10^7 PFU/mouse/i.p). Group(5): rats received DEN then treated with Doxorubicin at experimental week 10 at a dose level (2 mg/kg b.wt/i.p) and with Hitchner B1 at experimental week 10 at a dose levels (10^7 PFU/mouse/i.p). All animals were sacrificed after the end of experiment. DEN induced HCC showed elevation in serum ALT, AST, ALP, GGT, bilirubin. Also, resulted in upregulation in serum IL-6 and TNF- α ,On the other hand resulted in a significant decreased in serum Albumin, total protein compared with control group. Treatment with doxorubicin or/ and NDV showed that, all the parameters changed towards normal control group. The obtained results confirmed doxorubicin and NDV can inhibit the proliferation of HCC cells through improving hepatocytes.
Keywords
Diethylnitrosamine; Hepatocellular carcinoma; doxorubicin; NDV
Statistics
Article View: 146
PDF Download: 204