Prevalence of Inducible Clindamycin Resistance and Nanotechnological Control of Staphylococcus aureus Clinical Isolates

Abdelmawgoud, Youssef E.; Abd El- Latif, Walaa; Fawzy, Nouran K.; Elnagdy, Sherif M.

doi:10.21608/ejbo.2021.47561.1576

Prevalence of Inducible Clindamycin Resistance and Nanotechnological Control of Staphylococcus aureus<.i> Clinical Isolates

Egyptian Journal of Botany

Article 7, Volume 62, Issue 1, January 2022, Page 73-84 PDF (1.16 MB)

Document Type: Regular issue (Original Article)

DOI: 10.21608/ejbo.2021.47561.1576

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Authors

Youssef E. Abdelmawgoud¹; Walaa Abd El- Latif²; Nouran K. Fawzy¹; Sherif M. Elnagdy ¹

¹Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt

²Microbiology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Abstract

STAPHYLOCOCCUS aureus (S. aureus) is one of the pathogens that is proposed to cause various infections in humans. Clindamycin is therefore an important medication identified in treating these infections, especially those of the skin and soft tissue. Inducible clindamycin resistance (ICR) is a complication that however arises during the treatment of S. aureus infections in humans. Thus, it is difficult to detect S. aureus strains expressing ICR, using standard susceptibility test methods. Recently, an increasing need existed to find alternatives to antibiotics. Moreover, nanoparticles, especially silver nanoparticles, were previously reported as potential alternatives for traditional antibiotics (or in combination with traditional antibiotics) against the emergence of bacterial multidrug- resistance (MDR). Hence, this research studied the prevalence of ICR among S. aureus clinical isolates and investigated the antibacterial effects of AgNPs solely and combined with clindamycin against these isolates to evaluate the acute toxicity of intraperitoneally administrated silver nanoparticles (AgNPs). Of the one hundred S. aureus isolates under studied over a period of one year, 70 were identified as MRSA and 30 were MSSA. Results also revealed that the percentage of cMLSB, iMLSB, and MS phenotypes were 40%, 10%, and 9% respectively. Overall, 41% S. aureus isolates showed susceptibility to erythromycin. Additionally, both iMLSB and cMLSB phenotypes were the most predominated among MRSA isolates. Besides, AgNPs had strong antibacterial effects, with minimum inhibitory concentration (MIC)(1μg/ml) as well, in addition to a partial synergistic activity with clindamycin toward S. aureus. Based on these observations, the intraperitoneal administration of AgNPs was established as moderately toxic. Therefore, it may be recommended to use AgNPs as a potential treatment for infections caused by S. aureus.

Keywords

Clindamycin; Nanoparticles; Resistance; Silver; Staphylococcus aureus

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	Prevalence of Inducible Clindamycin Resistance and Nanotechnological Control of Staphylococcus aureus<.i> Clinical Isolates
Egyptian Journal of Botany
Article 7, Volume 62, Issue 1, January 2022, Page 73-84 PDF (1.16 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2021.47561.1576
View on SCiNiTO
Authors
Youssef E. Abdelmawgoud¹; Walaa Abd El- Latif²; Nouran K. Fawzy¹; Sherif M. Elnagdy ¹
¹Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt
²Microbiology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Abstract
STAPHYLOCOCCUS aureus (S. aureus) is one of the pathogens that is proposed to cause various infections in humans. Clindamycin is therefore an important medication identified in treating these infections, especially those of the skin and soft tissue. Inducible clindamycin resistance (ICR) is a complication that however arises during the treatment of S. aureus infections in humans. Thus, it is difficult to detect S. aureus strains expressing ICR, using standard susceptibility test methods. Recently, an increasing need existed to find alternatives to antibiotics. Moreover, nanoparticles, especially silver nanoparticles, were previously reported as potential alternatives for traditional antibiotics (or in combination with traditional antibiotics) against the emergence of bacterial multidrug- resistance (MDR). Hence, this research studied the prevalence of ICR among S. aureus clinical isolates and investigated the antibacterial effects of AgNPs solely and combined with clindamycin against these isolates to evaluate the acute toxicity of intraperitoneally administrated silver nanoparticles (AgNPs). Of the one hundred S. aureus isolates under studied over a period of one year, 70 were identified as MRSA and 30 were MSSA. Results also revealed that the percentage of cMLSB, iMLSB, and MS phenotypes were 40%, 10%, and 9% respectively. Overall, 41% S. aureus isolates showed susceptibility to erythromycin. Additionally, both iMLSB and cMLSB phenotypes were the most predominated among MRSA isolates. Besides, AgNPs had strong antibacterial effects, with minimum inhibitory concentration (MIC)(1μg/ml) as well, in addition to a partial synergistic activity with clindamycin toward S. aureus. Based on these observations, the intraperitoneal administration of AgNPs was established as moderately toxic. Therefore, it may be recommended to use AgNPs as a potential treatment for infections caused by S. aureus.
Keywords
Clindamycin; Nanoparticles; Resistance; Silver; Staphylococcus aureus


Statistics Article View: 845 PDF Download: 787

Prevalence of Inducible Clindamycin Resistance and Nanotechnological Control of Staphylococcus aureus<.i> Clinical Isolates