Enhancement of Immunogenicity and Protective Efficacy against Bovine Respiratory Disease Viruses (BVD, BoHV1, PI3, and BRS) Vaccine.
El-Bagoury, G., Mansour, M., Kassem, I. (2019). Enhancement of Immunogenicity and Protective Efficacy against Bovine Respiratory Disease Viruses (BVD, BoHV1, PI3, and BRS) Vaccine.. EKB Journal Management System, 36(1), 429-436. doi: 10.21608/bvmj.2019.176059
G.F El-Bagoury; Manal Mansour; Iman Kassem. "Enhancement of Immunogenicity and Protective Efficacy against Bovine Respiratory Disease Viruses (BVD, BoHV1, PI3, and BRS) Vaccine.". EKB Journal Management System, 36, 1, 2019, 429-436. doi: 10.21608/bvmj.2019.176059
El-Bagoury, G., Mansour, M., Kassem, I. (2019). 'Enhancement of Immunogenicity and Protective Efficacy against Bovine Respiratory Disease Viruses (BVD, BoHV1, PI3, and BRS) Vaccine.', EKB Journal Management System, 36(1), pp. 429-436. doi: 10.21608/bvmj.2019.176059
El-Bagoury, G., Mansour, M., Kassem, I. Enhancement of Immunogenicity and Protective Efficacy against Bovine Respiratory Disease Viruses (BVD, BoHV1, PI3, and BRS) Vaccine.. EKB Journal Management System, 2019; 36(1): 429-436. doi: 10.21608/bvmj.2019.176059

Enhancement of Immunogenicity and Protective Efficacy against Bovine Respiratory Disease Viruses (BVD, BoHV1, PI3, and BRS) Vaccine.

Benha Veterinary Medical Journal
Article 45, Volume 36, Issue 1, March 2019, Page 429-436  XML PDF (1.03 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.176059
View on SCiNiTO View on SCiNiTO
Authors
G.F El-Bagoury1; Manal Mansour2; Iman Kassem2
1Department of Virology, Faculty of Veterinary Medicine, Benha University
2Veterinary serum and vaccine research institute, Abbassia, Cairo
Abstract
A highly potent pneumo-4 vaccine of combined inactivated Bovine Viral Diarrhea Virus, Infectious Bovine Rhinotracitis Virus, Parainfluenza-3 Virus and Bovine Respiratory Syncytial Virus could play a valuable role in respiratory disease control program in calves. In this study we determined the influence of virus concentration by polyethylene glycol (PEG) precipitation, and the use of montanide oil & gel as adjuvants on the immune response of calves to the inactivated Pneumo-4 vaccine. Two batches of concentrated viruses’ vaccine were prepared, one adjuvanted with aluminum hydroxide gel and the other adjuvanted with oil. Both batches administered twice to six-month-old seronegative calves through intramuscular (IM) route. Different doses, 2ml, 2.5ml, and 3ml of the concentrated gel adjuvanted & 2.5ml,3ml and 3.5ml of concentrated oil vaccine were tested compared with 5ml for the commercial un-concentrated Pneumo-4 vaccine. The induced BVDV, IBRV, PI-3V and BRSV antibodies were followed up in the sera of vaccinated calves up to12M post vaccination (M.P.V.) using Serum Neutralization Test (SNT). The result revealed that concentration of viruses by PEG improve the quality of antigen used in the vaccine formulation to produce a good quality combined inactivated vaccine. Also, both concentrated adjuvanted vaccines gave a significant effect on all vaccinated animals but the oil adjuvanted vaccine was particularly better and effective than gel adjuvanted where oil adjuvanted vaccine induced early protective immune response and showed prolongation of antibody secretion in comparison to the Aluminum Hydroxide gel.
 
Keywords
Inactivated Pneumo-4 vaccine; polyethylene glycol; adjuvants; SNT
Statistics
Article View: 157
PDF Download: 179