IMMUNOLOGICAL STUDIES ON THE LOCAL INFECTIOUS BURSAL DISEASE VIRUS (IBD) ADAPTED ON SPECIFIC PATHOGEN FREE EMBRYONATED CHICKEN EGGS SPF (ECE) | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 23, Volume 89, Issue 2, July 2011, Page 709-729 PDF (601.49 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2011.176064 | ||||
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| Author | ||||
| NADIA M. IBRAHIM | ||||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt. | ||||
| Abstract | ||||
| Local isolate of (IBD) virus [EID50 106/ml] was propagated for three passages on (SPF) embryonated chicken eggs (EID50 106.5/ml) followed by three passages on SPF chickens (EID50 108/ml), then, for hundred and ten serial passages on SPF embryonated chicken eggs prepared and titrated (EID50 107/ml) after every 5 passages. The virus was inoculated into 21 days old chickens and observed for 21 days post-infection, then, challenged with virulent IBD virus. Passages (110, 77, 55) were used for preparation of live attenuated vaccines mild, intermediate, hot (IBDV) vaccines on SPF (ECE), respectively. The evaluation of the prepared vaccines was carried out for sterility, safety and potency. The potency was performed by measuring humoral and cellular immune response, as well as, protection percentage against virulent IBDV.The Efficacy of the prepared vaccine was estimated for up to six months. | ||||
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