EFFECT OF SOME AZOSPIRILLUM STRAINS AS A PARTIAL REPLACEMENT OF INORGANIC N FERTILIZERS ON YIELD AND QUALITY OF SUPERIOR GRAPEVINES | ||||
| Menoufia Journal of Plant Production | ||||
| Article 5, Volume 2, Issue 6, November and December 2017, Page 495-514 PDF (539.42 K) | ||||
| Document Type: original papers | ||||
| DOI: 10.21608/mjppf.2017.176384 | ||||
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| Authors | ||||
| F. F. Ahmed1; O. F. Dakhly2; M.A.M. Abada3; M. Kh. Uwakiem3 | ||||
| 1Hort. Dept., Fac. of Agric., Minia Univ., Egypt. | ||||
| 2Genetics. Dept., Fac. of Agric., Minia Univ., Egypt. | ||||
| 3Viticulture Res. Dept. Hort. Res. Inst. ARC, Giza, Egypt. | ||||
| Abstract | ||||
| About 91 mutants were obtained from exposing Azospirillum brasilense to different concentrations of Acriflavine mutagen and different time exposure. Out of those three new N microbial strains namely Methionineless, Threonineless and Tyrosineless were tested on Superior grapevines after their showed highest growth on artificial media. Superior grapevines were supplied with these microbial strains at 5 to 20 ml/vine and three slow release fertilizers (Methylene urea, Sulphur coated urea and urea formaldehyde) at 25 to 75% as partial replacement of the fast release N fertilizer namely ammonium nitrate during 2015 and 2016 seasons. Supplying Superior grapevines with N through 50 to 75 % inorganic N + 25 to 50% slow release N fertilizers (Methylene urea, Sulphur coated urea and urea formaldehyde) + bacteria strains (Methionineless, Threonineless and Tyrosineless) at 5 to 10 ml/vine proved to be very effective in stimulating all growth traits, leaf pigments and nutrients , berry setting %, yield and quality of the berries relative to using N as 100 % mineral N or when mineral N was added at 25%. An obvious reduction was observed on both nitrate and nitrite in the juice, with reducing percentages of mineral N from 100 to 25% as well as increasing percentages of the three release N fertilizers from 25 to 75% and the three bacterial strains from 5 to 20 ml/vine. The best bacterial strains in this respect were Methionineless, Theronineless and Tyrosineless, in ascending order. The best slow release fertilizer was methylene urea followed by sulphur coated urea. Amending Superior grapevines with N as 50% inorganic N (ammonium nitrate) + 50% slow release N fertilizer (methylene urea) + Tyrosineless at 10 ml/vine was responsible for promoting yield and fruit quality. | ||||
| Keywords | ||||
| Fast and slow release N fertilizers; bacterial strains; growth; yield; berries quality; Superior grapevines | ||||
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