Alteration of Oxidative Status in Rats Following Administration of Acrylamide
El-Sayed, H., Abd El- Halim, S., Morsy, G., El- Adawi, A. (2007). Alteration of Oxidative Status in Rats Following Administration of Acrylamide. EKB Journal Management System, 28(1), 397-403. doi: 10.21608/ejhm.2007.17669
Hanaa H. El-Sayed; Shawkia S. Abd El- Halim; Gehan M. Morsy; Ayman El-Sayed El- Adawi. "Alteration of Oxidative Status in Rats Following Administration of Acrylamide". EKB Journal Management System, 28, 1, 2007, 397-403. doi: 10.21608/ejhm.2007.17669
El-Sayed, H., Abd El- Halim, S., Morsy, G., El- Adawi, A. (2007). 'Alteration of Oxidative Status in Rats Following Administration of Acrylamide', EKB Journal Management System, 28(1), pp. 397-403. doi: 10.21608/ejhm.2007.17669
El-Sayed, H., Abd El- Halim, S., Morsy, G., El- Adawi, A. Alteration of Oxidative Status in Rats Following Administration of Acrylamide. EKB Journal Management System, 2007; 28(1): 397-403. doi: 10.21608/ejhm.2007.17669

Alteration of Oxidative Status in Rats Following Administration of Acrylamide

The Egyptian Journal of Hospital Medicine
Article 8, Volume 28, Issue 1, July 2007, Page 397-403  XML PDF (233.84 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2007.17669
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Authors
Hanaa H. El-Sayed1; Shawkia S. Abd El- Halim1; Gehan M. Morsy2; Ayman El-Sayed El- Adawi3
1National Nutrition Institute
2Women's college- Ain Shams University
3Home Economics college- Minufiya University
Abstract
Introduction: Acrylamide (ACR) is a known industrial neurotoxic and carcinogenic chemical in rodents. The recent discovery of acrylamide in wide variety of commonly consumed foods has energized research efforts worldwide to define toxic mechanisms.
Objective: The present study is carried out to investigate the effect of acrylamide administration on in vivo malondialdehyde (MDA, a product of lipid peroxidation), reduced glutathione (GSH) as well as copper and zinc superoxide dismutase enzyme activity (Cu/Zn SOD) of rats.
Material and Methods: Fourteen adult male Sprague Dawley rats were divided into two groups each containing "7" rats. Group 1 served as negative control fed on basal diet and group 2 (positive control) received basal diet and acrylamide (0.34g/ kg diet) for 11 days. Levels of MDA, GSH and activity of SOD were determined in liver, kidneys, brain, heart, testes, spleen and lungs of rats.
Results: ACR treatment significantly increased MDA in all organs; the highest increase was detected in testis (87.9%) and heart (71.5%) while the lowest one was found in kidneys (28.2%). On the other hand, GSH levels and SOD activities were significantly reduced in ACR treated rats. However, the reduction of GSH level ranged from 10.2% to 36.5 %.The inhibition of SOD activities were higher in testis (57.3%) and lungs (38.5%).
Conclusion: The present study showed that ACR exerts deteriorated effects on oxidative status of rats.
 
Keywords
Acrylamide –In vivo- Rats- Tissues – Lipid peroxidation – Reduced glutathione- Superoxide dismutase
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