PROTECTIVE EFFECT OF AQUAEOUS GREEN TEA EXTRACT ON TOXICITY CAUSED BY SODIUM NITRITE | |||||||||||||||||||||
Assiut Veterinary Medical Journal | |||||||||||||||||||||
Article 11, Volume 57, Issue 130, July 2011, Page 1-25 PDF (1.46 MB) | |||||||||||||||||||||
Document Type: Research article | |||||||||||||||||||||
DOI: 10.21608/avmj.2011.176858 | |||||||||||||||||||||
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Authors | |||||||||||||||||||||
GHADA M GOMAA1; EMAN A. ABD ELAZIZ2 | |||||||||||||||||||||
1Dept. of Forensic Medicine and Toxicology, Fac. of Vet. Med., Kafrelsheikh University, Egypt | |||||||||||||||||||||
2Dept. of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt | |||||||||||||||||||||
Abstract | |||||||||||||||||||||
Food additives are substances internationally added to food, this may be natural or synthetic. The safety of repeated exposure to permitted synthetic food additives (colorants or preservatives) has been questioned. Sodium nitrite (NaNO2, a food preservative agent) was used at 80 mg/kg/day and orally administered to the rats either alone or in conjugation with aqueous green tea extract (GTE, 10%) for 2 months. Blood and organs samples were collected for biochemical and histopathological examinations. Ingestion of NaNO2, induced a significant decline in blood glutathione reduced (GSH), plasma total antioxidant capacity (TAC) and glutathione -S- transferase (GST). Furthermore, significant increase was recorded in the level of serum lipid peroxide (MDA) and plasma nitric oxide (NO). Moreover, serum alanine transaminase (ALT), aspartate aminotransferase (AST) as well as bilirubin levels were increased significantly, while albumin level was decreased. Also, serum urea and creatinine levels were significantly increased. Plasma lactate dehydrogenase (LDH) and serum Creatine phosphokinase (CPK) activities were increased. Histopathological examination in sodium nitrite group rats revealed marked alterations in liver as apoptosis, periportal necrosis Sometimes, there were areas of focal necrosis which infiltrated with mononuclear cells giving the picture of granuloma like lesions, in addition to severe vacuolar degeneration. Also, kidney showed severe vacuolar degeneration and massive areas of tubular necrosis. Furthermore, heart showed necrosis of myocardial muscle cells. Fortunately, administration of green tea extract in conjugation with sodium nitrite showed significant amelioration of the investigated parameters and pathological changes. The results indicated that green tea extract has a potential to be developed as a preventive extract against sodium nitrite induced toxicity and the mechanism involved in the protection could be due to its antioxidant activity. | |||||||||||||||||||||
Keywords | |||||||||||||||||||||
Key words: Sodium nitrite; Green tea extract; Toxicity; Antioxidant activity | |||||||||||||||||||||
Full Text | |||||||||||||||||||||
Dept. of Forensic Medicine and Toxicology, Fac. of Vet. Med., Kafrelsheikh University, Egypt
PROTECTIVE EFFECT OF AQUAEOUS GREEN TEA EXTRACT ON TOXICITY CAUSED BY SODIUM NITRITE (With 2 Tables and 12 Figures)
By Ghada M Gomaa and Eman A. Abd Elaziz* * Dept. of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt. (Received at 2/5/2011)
التأثير الوقائى لمستخلص الشاى الأخضر على السميه التى تسببها نتريت الصوديوم
غاده محمود جمعه ، إيمان عبد العزيز
الاضافات الغذائية سواء الصناعية منها أو الطبيعية تضاف إلى الغذاء على مستوى العالم ولذلک فان سلامة التعرض المتکرر للاضافات الصناعية المسموح بها قيد الاختبارات. فى هذا البحث تم استخدام الصوديوم نتريت بجرعة 80 مج/کج من وزن الجسم / يوم للفئران البيضاء عن طريق الفم إما وحدها أو فى اقتران مع مستخلص الشاى الأخضر المائى (10%) لمدة شهرين. بعد هذه المدة تم تجميع عينات الدم والأعضاء للفحص البيوکيميائى والهستوباثولوجى. وقد وجد أن تجريع الصوديوم نتريت ادى إلى انخفاض ملحوظ فى مستوى کل من الجلوتاثيون، السعة الاجمالية لمضادات الأکسدة ، اليوريا والکرياتينين ، الکتيک ديهيدروجيناز ، کرياتنين فسفوکيناز فى الدم. وقد عضد الفحص الهستوباثولوجى النتائج حيث ظهرت التغيرات فى شکل موت للخلايا ونخر محيط للوريد البابى للکبد ، مخترقة بالخلايا وحيدة النواة فى بعض الاحيان مما يعطى ما يشبه الورم الحبيبى. بالاضافة إلى تنکس فجوى حاد. اما فى الکليه ، ظهرت مناطق من النخر الانبوبى والتنکس الفجوى الحاد. نخر عضلة القلب أيضاً ظهر نتيجة تجريع الصوديوم نتريت للفئران. ولکن لحسن الحظ، قد تحسنت جميع التغيرات السابقة سواء البيوکيميائية منها أو الهستوباثولوجية بالاستخدام المتزامن للشاى الأخضر کمصدر وحيد للشرب للفئران وهذه النتائج تشير إلى أهمية استخدامه کمستخلص وقائى ضد الاثار السمية الناتجة عن استخدام الصوديوم نتريت وذلک قد يکون لما يحتويه الشاى الاخضر من مضادات الاکسدة.
SUMMARY
Food additives are substances internationally added to food, this may be natural or synthetic. The safety of repeated exposure to permitted synthetic food additives (colorants or preservatives) has been questioned. Sodium nitrite (NaNO2, a food preservative agent) was used at 80 mg/kg/day and orally administered to the rats either alone or in conjugation with aqueous green tea extract (GTE, 10%) for 2 months. Blood and organs samples were collected for biochemical and histopathological examinations. Ingestion of NaNO2, induced a significant decline in blood glutathione reduced (GSH), plasma total antioxidant capacity (TAC) and glutathione -S- transferase (GST). Furthermore, significant increase was recorded in the level of serum lipid peroxide (MDA) and plasma nitric oxide (NO). Moreover, serum alanine transaminase (ALT), aspartate aminotransferase (AST) as well as bilirubin levels were increased significantly, while albumin level was decreased. Also, serum urea and creatinine levels were significantly increased. Plasma lactate dehydrogenase (LDH) and serum Creatine phosphokinase (CPK) activities were increased. Histopathological examination in sodium nitrite group rats revealed marked alterations in liver as apoptosis, periportal necrosis Sometimes, there were areas of focal necrosis which infiltrated with mononuclear cells giving the picture of granuloma like lesions, in addition to severe vacuolar degeneration. Also, kidney showed severe vacuolar degeneration and massive areas of tubular necrosis. Furthermore, heart showed necrosis of myocardial muscle cells. Fortunately, administration of green tea extract in conjugation with sodium nitrite showed significant amelioration of the investigated parameters and pathological changes. The results indicated that green tea extract has a potential to be developed as a preventive extract against sodium nitrite induced toxicity and the mechanism involved in the protection could be due to its antioxidant activity.
Key words: Sodium nitrite, Green tea extract, Toxicity, Antioxidant activity
INTRODUCTION
In relation to the toxicological limit, the FAO/WHO, Joint Expert Committee on Food Additive (JECFA) established acceptable daily intakes (ADIs) of 0-0.07 mg kg-1 body weight for sodium nitrite (expressed as nitrite ion) (WHO, 2003). It is widely used in food and drug industries as a preservative (Hill, 1991), and in medicine as antidote for cyanide poisoning (Filvo et al., 1993). Approximately 40% of absorbed nitrite is excreted unchanged in the urine and the fate of the rest 60% is not accurately know (Hill, 1991). The major metabolites of NaNO2 are nitric oxide and nitrosamine (Reisser et al., 1998). The later is highly carcinogenic and associated with a high risk of stomach, liver and esophagus carcinomas (Kim et al., 2002). Nitric oxide (free radicals) can cause DNA damage by inhibiting DNA synthesis and cell cycle arrest (Wink et al., 1991). The hazardous effect of NaNO2 derives from the reaction of nitrites with amines to produce nitrosamines, and with amides to produce nitrosamides. The toxic effects of nitrates and nitrites are well documented in mammalians, including impairment of reproductive function (Sleight et al., 1972), hepatotoxicity (Swann, 1975), dysregulation of inflammatory responses and tissue injury (Blanquat et al., 1983), growth retardation (Prasad, 1983), and endocrine disturbance (Jahries et al., 1986). A moderate and significant acceleration of leukemia development was observed in sodium nitrite treated mice (Ilntisky and Kolpakova, 1997). Also, NaNO2 inhibits a number of anti-tumor cytotoxic effector cell types as natural killer cells against pathogens and tumor cells (Abuharfiel et al., 2001). Children who ate more than 12 nitrite-cured hot dogs per month showed an increased risk of developing childhood leukemia (Peters et al., 1994). Anyway, nitrite when present at high concentration in blood, it can react with iron (III) of the hemoglobin, forming methaemoglobin which has no oxygen-carrying ability. This fatal disease is called methaemogobenemia (Sanchez-Echaniz et al., 2001).
Sodium nitrite was used at 10 mg/kg/day and orally administered to the rats for 30 days by Eman and Fahmy (2006) who reported that ingestion of NaNO2 significantly decreased RBCs%, WBCs%, hematocrit values%, Hb%, serum albumin, globulin and total protein contents in the treated rats while, serum alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), serum lactate dehydrogenase (LDH), creatine phosphokinase (CPK) were highly increased. Also, the authors observed elevation in triglycerides, total cholesterol and total lipids in serum and in the investigated tissues as well as hyperglycemia in food additive treated rats. Furthermore, sodium nitrite was found to significantly increase the lipid peroxidation and decrease the activities of antioxidant enzymes in liver such as superoxide dismutase, catalase and reduced glutathione level in rats received sodium nitrite orally for a month at dose rate of 300 mg/kg b.w. (kirshnamoorthy and Sangeetha, 2008). Concerning kidney function, it was reported that sodium nitrite produces significant elevation in serum urea and creatinine and significant decrease in renal urea and creatinine levels in rats dosed orally with 80 mg/kg b.w. for 3 months (Hanaa et al., 2009). Sodium nitrite intake studied by National Toxicology Program (2001) revealed that mean body weight at the highest dose was lower than that of control groups and showed the incidence of squamous cell papilloma and carcinoma of the forestomach in female mice. Szemes and szamado (1991) found that rats received sodium nitrite showed histopathological changes in striated muscles, liver and kidney. Also, Alam et al. (2005) observed that mice exposed to nitrosodibutylamine showed cell destruction and extensive necrosis. Green tea, from the steamed dried leaves of camellia sinesis, is widely consumed in eastern Asia. It comprises many types of catchins especially epigallocatchin-3-gallate (EGCG) which is the major polyphenol component and it is primarily responsible for the beneficial effects of green tea. Substantial evidence suggests that EGCG elicit anti-oxidant properties by attenuating the lipid peroxidation (LPO) caused by various forms of free radicals (Guo et al., 1999). It has been demonstrated that the chemically induced LPO in liver and kidney could be inhibited by the intake of tea catechins (Sano et al., 1995). In a recent study, supplementation of green tea extract (GTE) attenuated cyclosporine A-induced oxidative stress in rats (Mohamadin et al., 2005). Moreover, it can reduce the risk of colorectal and pancreatic cancers (Brown, 1999), inflammatory diseases and muscle necrosis (Benelli et al., 2002). In addition, green tea polyphenol might be a useful cancer chemopreventive agent in the human population (Lung et al., 2002). The aim of this work was to study the biochemical effects, antioxidant profile and histopathological features exerted by green tea extract (GTE) supplementation to sodium nitrite-induced toxicity in rats.
MATERIALS and METHODS
1. Chemicals NaNO2 (Sigma Aldrich, St Louis, MO) was applied as a freshly prepared solution. GTE was prepared from a hot-water extract of green tea (Twining and CO.LTD. LONDON) as described by Byung et al. (2009). Ten g of green tea leaves were soaked in 100 mL of boiling distilled water for 5 min. The solution was filtered to make 10 % green tea extract (GTE). This solution was provided to rats as their sole source of drinking water.
2. Kits Glutathione reduced (GSH), Total antioxidant capacity (TAC), glutathione -S- transferase (GST) lactate dehydrogenase (LDH), nitric oxide (NO) and lipid peroxide (MDA) kits were obtained from Biodiagnostic® Company. Alanine aminotransfrase (ALT), aspartate aminotransfrease (AST) kits were purchased from Biosystem® Company. Albumin, bilirubin, urea and creatinine were obtained from Human® Company. Creatine phosphokinase (CPK) kit was purchased from bioMerieux® Company.
3. Animals Forty mature healthy Sprague-Dawley rats of both sexes weighing 120-150 g (purchased from Animal House Colony, Giza, Egypt) were housed in stainless-steel cages with hard wood shavings as bedding. Animals were accommodated to the laboratory conditions for one-week before experiment. They were maintained on balanced ration and given water ad-libitum throughout the experimental period (2 months).
4. Experimental design The animals were divided into four groups (10 rats each) as follows:
(1) Group 1: Rats fed on the basal diet and normal water, left in normal conditions for 2 months which served as control group. (2)Group 2: Rats orally administered green tea extract (GTE) (10%) as their sole source of drinking water for 2 months and served as GTE group.
(3) Group 3: Rats were treated with sodium nitrite by gavages at a dose of 80 mg/kg body weight daily as previously described by (Hanaa et al., 2009) for 2 months and served as Sodium nitrite - intoxicated group.
(4) Group 4: Rats were orally administered both GTE as their sole source of drinking water, sodium nitrite by gavages at a dose of 80 mg/kg body weight daily for 2 months and served as Sodium nitrite–GTE group.
4.1. Biochemical analysis Two blood samples were collected from each animal via the retro-orbital venous plexus after fasting for 12 h. The first blood sample was collected on EDTA for estimation of GSH in whole blood. TAC, GST, LDH and NO were estimated in plasma. The second blood sample was left to clot then centrifuged at 5000 rpm for 10 min to separate serum for measuring liver ALT, AST, albumin and bilirubin, kidney urea and creatinine functions in addition to CPK and MDA activities. GSH content was estimated according to Beutler et al. (1963). TAC was determined according to Koracevic et al. (2001). GST was estimated according to Habig et al. (1974), in addition to oxidative biomarkers (NO) which was determined according to Montgomery and Dymock (1961) and MDA was determined according to method described by Ohkawa et al. (1979). LDH activity was determined as previously described by Raabo (1963). ALT and AST activities were determined according to the method described by Reitman and Frankel (1957). Albumin was measured as previously reported by Tietz (1990), whereas bilirubin, urea and creatinine were estimated as by the method of Henry (1974). CPK was performed according to the method of Oliver (1955).
4.2. Histopathological examination Specimens from liver, kidney and heart were preserved in 10% buffer neutral formalin and processed through paraffin embedding technique. Sections of about 5-7 microns thickness were cut and stained with hematoxylin and eosin (H&E) for histopathologic examination according to Culling (1983). 5. Statistical analysis Statistical analysis of data was conducted using statistical analysis system SAS (2001).
RESULTS
Table 1: The effect of sodium nitrite (80 mg/kg b.w. daily) and aqueous green tea extract (10%) on some antioxidant and oxidative biomarkers of rats.
- Values are mean ± standard error. - Means in a row without a common letter differ significantly (P<0.05).
Table 2: The effect of sodium nitrite (80 mg/kg b.w. daily) and aqueous green tea extract (10%) on liver, kidney and heart functions parameters of rats.
- Values are mean ± standard error. - Means in a row without a common letter differ significantly (P<0.05)
Fig. 1: Liver (group2), showing apoptotic figures which were collected either in groups or in individual scattered in hepatic parenchyma. (H&E) (10x20).
Fig. 2: Liver (group2), showing periportal necrosis of hepatic parenchyma. (H&E) (10x20).
Fig. 3: Liver (group2), showing focal area of necrosis heavily infiltrated with mononuclear cells giving the picture granuloma like lesion. (H&E) (10x20).
Fig. 4: Liver (group2), showing midzonal mononuclear cell infiltration which accompanied with peripheral single cell necrosis. (H&E) (10x20).
Fig. 5: Liver (group2), showing severe vacuolar degeneration in the form of ill-defined vacuoles leading to rarefaction of cytoplasm with pycknotic nuclei. (H&E) (10x20).
Fig. 6: Kidney (group2), showing severe vacuolar degeneration in the form of ill-defined vacuoles with pycknosis of nuclei of some renal tubular cells. (H&E) (10x20).
Fig. 7: Kidney (group2), showing massive areas of tubular necrosis (H&E) (10x20).
Fig. 8: Kidney (group2), showing slight thickening of basement membrane of glomerulus and pycknosis of nuclei of mesangial cells. (H&E) (10x20).
Fig. 9: Heart (group2), showing necrosis of myocardial muscle cells. The necrotic area was infiltrated with mononuclear cells including macrophages engulfing necrotic cells (myophagia). (H&E) (10x20).
Fig. 10: Liver (group3), showing sinusoidal dilatation and multiple microvacuoles in cells. (H&E) (10x20).
Fig. 11: Kidney (group3), showing slight vacuolar change in renal epithelial. (H&E) (10x20).
Fig. 12: Kidney (group3), showing pycknotic nuclei of some cells of renal tubules. (H&E) (10x20).
DISCUSSION
Oxidative stress has been implicated in the development of many toxic conditions. Sodium nitrite and other additives may react with amines of the foods in the stomach and produce nitrosamines and free radicals. Such products may increase lipid peroxidation, which can be harmful to different organs including liver, kidney and heart (Choi et al., 2002). On the other hand, these free radicals, known to cause oxidative stress, can be prevented or reduced by dietary natural antioxidants through their capacity to scavenge these products (Aruoma, 1998). In present study, a number of antioxidant biomarkers were examined and the results are summarized in Table 1. The administration of sodium nitrite orally to rats at dose of 80 mg/kg body weight daily for 2 months, resulted in a dramatic decline (P<0.05) in blood GSH, plasma TAC and GST; these results are supported by previous data obtained by (kirshnamoorthy and Sangeetha, 2008). These results may be attributed to the observed induction of lipid peroxidation (Shahjahan et al., 2005). Glutathione plays a crucial role in the cellular antioxidant defense system by scavenging free radicals and other reactive oxygen species, removing hydrogen and lipid peroxides and preventing oxidation of biomolecules (Wu et al., 2004). The oxidative stress caused by sodium nitrite can lead to significant depletion of GSH when compared with control animals. The downfall of blood GSH content in this study might be due to its enhanced utilization for scavenging free radicals. Moreover, estimation of total antioxidant capacity (TAC) may provide more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present in plasma and body fluids (Koracevic et al., 2001).
In the current study, administration of sodium nitrite elicited a significant increase (P<0.05) in the level of oxidative biomarkers including plasma NO and serum MDA compared to normal control group. MDA is an end product of lipid peroxidation, and it is considered a late biomarker of oxidative stress and cellular damage (Carampin et al., 2003). Furthermore, there is a significant increase in plasma nitric oxide free radical (NO∙) due to its generation from the nitrite by nonenzymatic method and O∙ formation which found in acidic environment such as stomach and oral cavity (Mcknight et al., 1997). Both NO∙ and oxygen radicals could react further to produce other oxidant and nitro compounds such as peroxynitrite which is a powerful oxidant and causes tissue damage (Teppema et al., 2002).
Histopathological findings confirmed the toxic effects of sodium nitrite and are consistent with biochemical determination. In sodium nitrite group, there were marked alterations in liver, kidney and heart. Sodium nitrite treated animals showed increased apoptotic figures. The apoptotic cells were collected either in groups or in individuals scattered in hepatic parenchyma. The necrotic cells showed more acidophilic cytoplasm and pyknotic nuclei (Fig.1). The increase in apoptotic figures may be due to induction of oxidative stress and mitochondrial alteration (Fladmark et al., 1999).
Hepatic necrosis has been the most frequently reported lesion associated with sodium nitrite induced liver damage. Furthermore, liver of the same animals showed periportal necrosis of hepatic parenchyma mostly observed in portal areas in which the cytoplasm became more acidophilic with pyknosis or completely disappearance of nuclei (Fig.2). In other cases, there were focal areas of necrosis heavily infiltrated with mononuclear cells giving the picture of granuloma like lesions (Fig3). These findings are in agreement with that mentioned by Hassan et al. (2009) who recorded liver necrosis induced by sodium nitrite and attributed the liver damage to the toxic effect of nitros compounds formed in acidic environment of stomach causing hepatic necrosis. These obtained results are confirmed by the biochemical data which showed significant increase in AST, ALT and bilirubin in serum of sodium nitrite treated rats indicating hepatic disorder and damage.
Our results also indicated an inhibitory effect of sodium nitrite on albumin synthesis, this may be due to stimulation of thyroid and adrenal glands which can lead to a blockade in protein synthesis, fat break down, increased rate of free amino acids and decreased protein turnover (Eremin and Yocharina, 1981). In addition, the release of NO∙ can inhibit protein synthesis through inhibiting oxidative phosphorylation process and the availability of the energy source for protein synthesis (Anthony et al., 1994). Another histopathological finding in sodium nitrite group of rats was mononuclear cell infiltration in hepatic parenchyma either perivascular, periportal or midzonal which sometimes accompanied with peripheral single cell necrosis (Fig.4). This finding is in accordance with the view that the NO∙ originally known to be important in the modulation of tissue inflammation (Rees et al., 1990). Moreover, Sarsour and Hassuneh (2001) reported that sodium nitrite causes inflammatory reaction and infiltration of inflammatory cells that subsequently release large quantities of potential oxidants as H2O2 that might induce damage to surrounding tissue and cells. Alternatively, the hepatic cells in some animals of sodium nitrite group of rats showed severe vacuolar degeneration in the form of ill-defined vacuoles leading to rarefaction of cytoplasm with pyknotic nuclei (Fig5). This finding indicates increased production of free radicals especially reactive oxygen species that attack the cell membranes and other molecules inside the cells.
In present study the results of assessment of kidney function (urea and creatinine levels) as recorded in Table 2, indicated that serum urea and creatinine levels increased significantly in sodium nitrite group, suggesting an impairment of kidney function. These results were in agreement with those obtained by Eman and Fahmy (2006) and correlated with the results of the histopathological examination of kidney of sodium nitrite group of rats.
The kidney showed severe vacuolar degenerative changes in the form of ill-defined vacuoles mostly observed towards the luminal border of tubules together with pyknosis of nuclei of some renal tubular cells (Fig.6). Some rats showed massive areas of tubular necrosis (Fig.7) and very few revealed slight thickening of the basement membrane of glomeruli and pyknosis of nuclei of mesangial cells (Fig.8). Sodium nitrite induced renal toxicity was in accordance with that obtained by (Hanaa et al., 2009), this toxicity may be attributed to increased generation of NO∙ which is the major metabolite of sodium nitrite (Reisser et al., 1998) that leads to increased lipid peroxidation (Goligosky et al., 2002) or may be due to direct effect of sodium nitrite which alters transport of sodium and chloride in the distal nephron and may also adversely affect the renal function (Zaki et al., 2005) or through changes in the threshold of tubular re-absorption, renal blood flow and glomerular infiltration rate (Zurovsky and Haber, 1995).
In current study, the heart function markers (CPK and LDH) increased significantly indicating cellular damage of the heart. This may be due to the increase in the production of free radicals. The histopathological findings of the heart in sodium nitrite group of rats confirmed these biochemical results. There was focal necrosis of myocardial muscle cells in which the necrotic cells had more acidophilic cytoplasm with pyknotic nuclei and loss of muscle fiber striations. The necrotic area was infiltrated with mononuclear cells including macrophages engulfing necrotic cells (myophagia) (Fig.9).
The main components of green tea are catechins, which have a polyphenol structure, including [(−)-epigallocatechin-3-gallate (EGCG)], [(−)-epigallocatechin (EGC)], [(−)-epicatechin-3- gallate (ECG)] and [(−)-epicatechin (EC)]. All these catechins have strong antioxidant activity (Higdon & Frei, 2003) and are considered as potent scavengers of reactive oxygen species (ROS) and nitrogen species such as superoxide, lipid peroxide, peroxynitrite, hydroxyl radicals and nitric oxide produced by various chemicals (Schroeder et al., 2003). Green tea prevents the loss of lipophilic antioxidant a-tocopherol, by repairing tocopheryl radicals and protection of the hydrophilic antioxidant ascorbate (Skrzydlewska et al., 2002). Therefore, it may decrease the concentration of lipid free radicals and terminate initiation and propagation of lipid peroxidation (Guo et al., 1999). Catechins have beneficial effects in prevention of cardiovascular diseases including LDL oxidative susceptibility, serum lipids and lipoprotein concentrations (Wan et al., 2001).
The administration of green tea extract (10%) in conjugation with sodium nitrite ameliorated the nitrite adverse effects as evidenced by restored TAC as well as increased GSH level and GST activity. In addition to significant reduction in NO and MDA levels when compared with normal control and sodium nitrite group. Also, supplementation of sodium nitrite intoxicated rats with green tea extract declined the AST, ALT and bilirubin levels when compared to normal control and sodium nitrite rats. Moreover, albumin level was significantly increased in Sodium nitrite-GTE rats. The results of histopathological examination were in accordance with the liver function biochemical results. There was marked improvement in liver upon treatment of sodium nitrite intoxicated rats with green tea. The most hepatic changes induced by sodium nitrite disappeared except some sinusoidal dilatation and multiple microvacuoles in hepatic cells (Fig.10). These findings simulate those of Ostrowska et al. (2004) who detected that green tea protected liver and caused disappearance of necrotic areas induced by ethanol intoxication. Also green tea protected hepatic cells against tamoxifen intoxication (Elbeshbishy, 2005) and against microcystin –LR induced hepatotoxicity (Xu et al., 2007). More over, Relja et al. (2010) proved that green tea reduced liver injury and necrosis induced by Hemorrhage resuscitation (H-R) in rats. The apoptosis of hepatic cells was decreased when compared with sodium nitrite group. This finding was in agreement with Hockenberg et al. (1990) who mentioned that B-CL-2 protein residue in the mitochondrial outer membrane, has been implicated in the regulation of mitochondria permeability transition (MPT) and release of apoptogenic proteins from mitochondria into cystol, B-CL-2 protein is an important gene product to control apoptosis, and it can inhibit apoptosis through blocking apoptotic signal transmitted system. The present study, suggests that the modulation of B-CL-2 protein expression by green tea might be an important factor in signal transduction mediating the protective effect against sodium nitrite induced apoptosis of hepatic cells.
In current study, supplementation of sodium nitrite intoxicated rats with green tea extract restored the normal urea level as well as declined creatinine level when compared to normal control and sodium nitrite rats. These obtained data were confirmed by histopathological examination of kidney of rats treated with both sodium nitrite and green tea extract. There was marked improvement in kidney picture and absence of most pathological alterations which were detected in sodium nitrite rat group except there was slight vacuolar change in renal epithelium (Fig.11) and pyknotic nuclei of some cells of renal tubules (Fig.12). Similar results were detected by Ozer et al. (2008) who reported that EGCG supplementation improved the histopathological pictures in kidney of rats exposed to sevoflurane where green tea decreased renal degeneration and caused disappearance of cortical necrosis in kidney tissues. This protection may be attributed to phosphorylation and activation of endothelial nitric oxide synthase in endothelial cells through modulation of protein kinase C, A signaling pathways by green tea resulting in endothelial dependant vasorelaxtion (Lorenz et al., 2004)
In the present study, heart function markers (serum CPK and LDH) showed marked decrease in the group of rats received both sodium nitrite and green tea, these biochemical results are in accordance with histopathological picture of the heart. There was absence of necrosis and the heart showed more or less normal appearance except congestion.
In the present study, GTE rats did not show any significant changes in the majority of the estimated parameters and histopathological examinations except AST level showed significant decrease when compared to normal control rats. Further more, GTE rats showed significant increase in GSH and TAC activities as well as significant decrease in NO and MDA levels, while there is no significant change in GST level when compared with normal control rats. In conclusion, from the results achieved, it can be concluded that the administration of green tea extract has an extremely beneficial role in overcoming the adverse effects of ingestion of sodium nitrite, which is probably through its excellent antioxidant properties and enhancing the antioxidant potency of studied organs cells and organelles.
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(1963): Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61: 882-888. Blanquat, D.G.; Fritsch, F. and Cazotles, C. (1983): Effect of dietary nitrate and nitrite on experimentally induced inflammation in the rat. International Journal of Tissue Reactions, 27: 173-180. Brown, M. (1999): Green tea extract and its possible role in the prevention of cancer. Alternative Medicine Reveiw, 4: 360-370. Byung, C.; Hyun, H.; Jong, H.; Byoung, R. and Hyun, L. (2009): The protective effects of green tea extract against L-arginine toxicity to cultured human mesangial cells. Korean Medical Science, 24 (Suppl 1), S204-9 ISSN: 1011-8934. Carampin, P.; Rosan, S.; Dalzoppo, D. and Zagotto, G. (2003): Some biochemical properties of melatonin and the characterization of a relevant metabolite arising from its interaction with H2O2. Journal of Pineal Research, 34(2): 134-42. Choi, S.Y.; Chung, M.J. and Sung, N.J. (2002): Volatile N-nitrosamine inhibition after intake of Korean green tea and Maesil (Prunus mume SIEB. et ZACC.) extracts with an amine-rich diet in subjects ingesting nitrate. Food and Chemical Toxicology, 40: 949-957. Culling, C.F.A. (1983): Handbook of Histopathologic and Histochemical Technique, 3rded. Butter Worth London, Boston: 214. El-Beshbishy, H.A. (2005): Hepatoprotective effect ofgreen tea (Camellia sinensis) extract against Tamoxifen induced liver injury in rats. Journal of Biochemistry and Molecular Biology, 38(5): 563-570. Eman, G.E. and Fahmy, G.G. (2006): Management the action of sodium nitrite on albino rats by aqueous garlic extract.Research Journal of Medicine and Medical Sciences, 1(3): 85-89. Eremin, Y.N. and Yocharina, M.C. (1981): Effect of nitrites on the state of thyroid gland in iodine deficiency and different diets. Vopr. Pitan., 5: 60-62. Filvo, V.A.; Ivin, B.A. and Bandman, A.L. (1993): Hramful chemical substances: Elements in group IIV of the periodic Table and their inorganic compounds. Ellis Horwood, New York. Fladmark, K.E.; Brumgum, O.T.; Hovland, R.; Boe, R.; Gjertsen, B.T.; Zhivotovsky, B. and Døskeland, S.O. (1999): Ultra rapid caspase-3 dependent apoptosis induction by serine /theonine phosphatase inhibitors. Cell Death Differ. 6(11): 1099-1108. Goligorsky, M.S.; Brodsky, S.V. and Noiri, E. (2002): Nitric oxide in acute renal failure: NOS versus NOS. Kidney Int., (3): 855-61. Guo, Q.; Zahao, B.; Shen, S.; Hou, J.; Hu, J. and Xin, W. (1999): ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers. Biochimica et Biophysica Acta, 1427: 13-23. Habig, W.H.; Pabst, M.J. and Jakoby, W.B. (1974): Glutathione- S-transferases. The first enzymatic step in mercaptouric acid formation. Journal of biological chemistry, 249: 7130-7139. Hanaa, A.; Sherif, M.; Rajiv, L.; Augusta, F.; Madhwa, H. and Allal, O. (2009): In vivo evidence of hepato- and reno-protective effect of garlic oil against sodium nitrite-induced oxidative stress. International Journal ofBiological Sciences, 5 (3): 249-255. Hassan, H.A.; El-Agamy, S.M.; Gaur, R.; Fernando, A.; Raj, M.H. and Quhtit, A. (2009): In vivo evidence of hepato and reno-protective effect of garlic oil against sodium nitrite induced oxidative stress. International Journal of Biological Science, 5(3): 249-255. Henry, R.J. (1974): Principles and Techniques. Clinical Chemistry, 2nd ed. Harper and Row, 525. Higdon, J.V. and Frei, B. (2003): Tea catechins and polyphenols: health effects metabolism and antioxidant functions. Critical Reviews in Food Science and Nutrition, 43 (1): 89–143. Hill, M. (1991): Nitrates and nitrites in food and water. Ellis Horwood, New York, (pp. 131-132). Hockenberg, D.; Nunez, G.; Milliman, S.; Schreiber, R.D. and Korsmeyer, S.J. (1990): Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature, 348 (9299): 334-336. Ilntisky, A.P. and Kolpakova, A.S. (1997): The enhancing effect of sodium nitrite on virus-induced leukemia in mice. Cancer Detection Prevention, 21: 312. Jahries, G.; Hesse, V.I.; Schone, L.H. and Mehnert, E. (1986): Influence of nitrates and plant goitrgens on thyroid hormone, somated in status and growth of swine. JournalofVeterinary Medicine, 41: 528-530. Kim, H.J.; Chang, W.K.; Kim, M.K.; Lee S.S. and Choi, B.Y. (2002): Dietary factors and gastric cancer in Korea: Case-control study. International Journal of Cancer, 97: 531-535. Kirshnamoorthy, P. and Sangeetha, M. (2008): Protective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats. Indian Journal of Biochemistry and Biophysics, 45: 206-208. Koracevic, G.; Djordjevic,V.; Andrejevic, S. and Cosic, V. (2001): Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology, 54: 356-361. Lorenz, M.; Wessler, S. and Follmann, E. (2004): A constituent of green tea, epigallocatechin-3-gallate, activates endothelial nitric oxide synthase by a phosphatidylinositol-3-OH-kinase-, cAMP-dependent protein kinase-, and Akt-dependent pathway and leads to endothelial-dependent vasorelaxation. J. Biol. Chem. (13): 6190-6195. Lung, H.L.; Ip, W.K.; Wong, C.K.; Mak, N.K.; Chen, Z.Y. and Leung, K.N. (2002): Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line. Life Science, 72 (3): 257–268. Mcknight, G.M.; Smith, L.M.; Drummond, R.S.; Duncan, C.W.; Golden, M. and Benjamin, N. (1997):Chemical synthesis of nitric oxide in the stomach from dietary nitrate in humans. Gut, 40: 212-214 Mohamadin, A.; El-Beshbishy, H. and El-Mahdy, M. (2005): Green tea extract attenuates cyclosporine A-induced oxidative stress in rats. Pharmacological Research, 51: 51-57. Montgomery, H.A.C. and Dymock, J.F. (1961): Determination of nitric oxide. Analyst, 86: 414. National Toxicology Program, (2001): Toxicology and carcinogenesis studies of sodium nitrite CAS No. 7632-00-0 in F344/N rats and B6C3F1 mice drinking water studies. Nat. Toxicol. Program Tech. Rep. Ser., 495: 7-273. Ohkawa, H.; Ohishi, N. andYagi, K. (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 95: 351–358. Oliver, I.T.A. (1955): Spectrophotometric method for the determination of creatine phosphokinase and myokinase. Biochemical Journal, 61:116-122. Ostrowska, J.; Luczaj, W.; Kasacka, I; Rózanski, A. and Skrzydlewska, E. (2004): Green tea protects against ethanolinduced lipid peroxidation in rat organs. Alcohol, 32: 25-32. Özer, A.B.; Kaman, D.; Erhan, Ö.L. and Özer, S. (2008): EGCg supplementation improves oxidant and antioxidant status in kidney of rats exposed to sevoflurane. Fırat. Tıp. Derg., 13: 1. Peters, J.M.; Preston-Martin, S.; London, S.J.; Bowman, J.D.; Buckley, J.D. and Thomas, D.C. (1994): Processed meats and risk of childhood leukemia. Cancer Causes and Control, 5: 195-202. Prasad, J. (1983): Effect of high nitrate diet on thyroid glands in goats. Indian Journal of Animal Sciences, 53: 791-794. Raabo, E. (1963): Determination of serum lactic dehydrogenase by the tetrazolium salt method. Scandinavian Journal of Clinical and Laboratory Investigation, 15: 233-238. Rees, D.D.; Cellek, S.; Palmer, R.M. and Moncada, S. (1990): Dexamethasone perevents the induction by indotoxin of a nitric oxide synthase and the associated effects on vascular one: an insight into endotoxine shock. Biochem. Biophys. Res. Commun., 173: 541-547. Reisser, D.; Lagadec, P.; Arnould, L.; Arnould, N.; Maupoil, V.; Pinard D. and Eannin, J.F. (1998): Nitric oxide inhibits proliferation but increases life-span of Tlymphocytesin tumor-bearing rats. Cancer Immunology, Immunotherapy, 46: 160-166. Reitman, S. and Frankel S. (1957): A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American Journal of Clinical Pathology, 28: 56-63. Relja, B.; Tottel, E.; Bteig, L.; Henrich, D.; Schneider, H.; Marzi, I. and Lehnert, M. (2010): Effects of green tea catechins on the pro-inflammatory response after haemorrhage/resuscitation in rats. Br. J. Nutr., 4: 1-7. Sanchez-Echaniz, J.; Benito-Frnandez, J. and Mintegui-Raso, S. (2001): Methemoglobinemia and consumption of vegetables in infants. Pediatrics, 107: 1024-1028. Sano, M.; Takahashi, Y.; Yoshino, K.; Shimoi, K.; Nakamura, Y.; Tomita, I.; Oguni, I.; and Konomoto, H. (1995): Effect of tea (Camellia sinensis L.) on lipid peroxidation in rat liver and kidney: a comparison of green and black tea feeding. Biological and Pharmaceutical Bulletin, 18 (7): 1006–1008. Sarsour, A. and Hassuneh, N.E. (2001): The effect of sodium nitrite on some parameters of the immune system. Food Chem Toxicol., 39 (2): 119-24. SAS (2001): Statistical Analysis System. Users Guide: statistics, (SAS Institute, CaryNorth Carolina). Schroeder, P.; Klotz, L.O. and Sies, H. (2003): Amphiphilic properties of (−) epicatechin and their significance of protection of cells against peroxynitrite. Biochemical and Biophysical Research Communications, 307 (1): 69–73. Shahjahan, M.; Vani, G. and Shyamaladevi, C.S. (2005): Effect of Solanum trilobatum on the antioxidant status during diethyl nitrosamine induced and phenobarbital promoted hepatocarcinogenesis in rat. Chemo- Biological Interaction, 156: 113-123. Skryzdlewska, E.; Ostrowska, J.; Stankiewicz, A. and Fabisszewski, R. (2002): Green tea as a potent antioxidant in alcohol intoxication. Addiction Biology, 7: 307-314. Sleight, S.D.; Sinha, D.P. and Uzoukwu, M. (1972): Effect of sodium nitrate on reproductive performance of pregnant sows. Journal of the American Veterinary Medical Association, 161: 819-823. Swann, P.F. (1975): The toxicology of nitrate, nitrite and N-nitroso compounds. Journalof theScienceofFoodandAgriculture, 26: 1761-1770. Szemes, Z. and Szamado, I. (1991): Comparasion of histological effect of electrocoagulation and Nd-YAG laser coagulation in intact and timorous rat tissue. Acta Chir. Hung., 32: 245-251. Teppema, L. J.; Nieuwenhuijs, D.; Sarton, E.; Romberg, R.; Wards, D.S. and Dahan, A. (2002): The Journal of Physiology, 549: 931-938. Tietz, N.W. (1990): Clinical guide to laboratory tests. 2nd ed. Philadelphia:WB Saunders, 26-29. Wan, Y.; Vinson, J.; Etherton, T.; Porch, J.; Lazarus, S. and Kris-Etherton, P. (2001): Effects of cocoa powder and dark chocolate on LDL oxidative susceptibility and prostaglandin concentration in humans. American Society for Clinical Nutrition, 74: 596-602. Wink, D.A.; Kasprzak, K.S.; Maragos, C.M.; Elepuru, R.K.; Misra, M.; Dunams, T.M.; Cebala, A.; Koch, W.H.; Andrews, A.W.; Allen, J.S. and Keefer, L.k. (1991): DNA deaminating ability and genotoxicity of nitric oxide and its progenitors. Science, 254: 1001- 1003. World Health Organization, (2003): Safety evaluation of certain food additives. Fifty-fourth Report of the Joint FAO/WHO Committee on Food Additives. Food Additives Series No. 50. Genera: WHO. Wu, G.; Fang, Y.Z.; Yang, S.; Lupton, J.R. and Turner, N.D. (2004): Glutathione metabolism and its implications for health. Journal of Nutrition, 134: 489-492. Zaki, A.; Chaoui, A.A.; Chait, A.; Aboussaouira, T.; Zarrouk, K. and Himmi, T. (2005): Effect of inorganic nitrates on the morphofunctional condition of the kidney in the rat. Therapie., 60(1): 75-9. Zurovsky, Y. and Haber, C. (1995): Antioxidants attenuate endotoxin- generation induced acute renal failure in rats. Scandinavian Journal of Urology and Nephrology, 29: 147-154. Xu, C.; Qunshu, W.; Qun Qiu; Zhao, Q. and Cao, J. (2007): Protective effects of green tea polyphenols against subacute epatotoxicity induced by microcystin-LR in mice. Environmental Toxicology and Pharmacology 24: 140–148.
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References | |||||||||||||||||||||
REFERENCES Abuharfiel, N.; Sarsour, E. and Hassuneh, M. (2001): The effect of sodium nitrite on some parameters of the immune system. FoodandChemical Toxicology,39: 119-124.
Alam, A.; Singha, L.I. and Singh, V. (2005): Molecular characterization of tumor associated antigen in mice exposed to a hepatocarcinogenesis. Mol. Cell. Biochem., 271: 177-188.
Anthony, M.L.; Gatland, K.P.; Beddell, C.R. and Lindon, J.K. (1994): Studies on the biochemical toxicology of uranyl nitrate in the rat. Archives of Toxicology, 68(1): 43-53.
Aruoma, O.I. (1998): Free radicals, oxidative stress and antioxidants in human health and disease. Journal of the American Oil Chemists' Society, 75: 199-212.
Benelli, R.; Vene, R.; Bisacchi, D.; Garbisa, S. and Albini, A. (2002): Anti-invasive effects of green tea polyphenol epigallocatechin-3-gallate, a natural inhibitor of metallo and serine protease. Biological Chemistry, 383: 101-105.
Beutler, E.; Duron, O. and Kelly, M.B. (1963): Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61: 882-888.
Blanquat, D.G.; Fritsch, F. and Cazotles, C. (1983): Effect of dietary nitrate and nitrite on experimentally induced inflammation in the rat. International Journal of Tissue Reactions, 27: 173-180.
Brown, M. (1999): Green tea extract and its possible role in the prevention of cancer. Alternative Medicine Reveiw, 4: 360-370.
Byung, C.; Hyun, H.; Jong, H.; Byoung, R. and Hyun, L. (2009): The protective effects of green tea extract against L-arginine toxicity to cultured human mesangial cells. Korean Medical Science, 24 (Suppl 1), S204-9 ISSN: 1011-8934.
Carampin, P.; Rosan, S.; Dalzoppo, D. and Zagotto, G. (2003): Some biochemical properties of melatonin and the characterization of a relevant metabolite arising from its interaction with H2O2. Journal of Pineal Research, 34(2): 134-42.
Choi, S.Y.; Chung, M.J. and Sung, N.J. (2002): Volatile N-nitrosamine inhibition after intake of Korean green tea and Maesil (Prunus mume SIEB. et ZACC.) extracts with an amine-rich diet in subjects ingesting nitrate. Food and Chemical Toxicology, 40: 949-957.
Culling, C.F.A. (1983): Handbook of Histopathologic and Histochemical Technique, 3rded. Butter Worth London, Boston: 214.
El-Beshbishy, H.A. (2005): Hepatoprotective effect ofgreen tea (Camellia sinensis) extract against Tamoxifen induced liver injury in rats. Journal of Biochemistry and Molecular Biology, 38(5): 563-570.
Eman, G.E. and Fahmy, G.G. (2006): Management the action of sodium nitrite on albino rats by aqueous garlic extract.Research Journal of Medicine and Medical Sciences, 1(3): 85-89.
Eremin, Y.N. and Yocharina, M.C. (1981): Effect of nitrites on the state of thyroid gland in iodine deficiency and different diets. Vopr. Pitan., 5: 60-62.
Filvo, V.A.; Ivin, B.A. and Bandman, A.L. (1993): Hramful chemical substances: Elements in group IIV of the periodic Table and their inorganic compounds. Ellis Horwood, New York.
Fladmark, K.E.; Brumgum, O.T.; Hovland, R.; Boe, R.; Gjertsen, B.T.; Zhivotovsky, B. and Døskeland, S.O. (1999): Ultra rapid caspase-3 dependent apoptosis induction by serine /theonine phosphatase inhibitors. Cell Death Differ. 6(11): 1099-1108.
Goligorsky, M.S.; Brodsky, S.V. and Noiri, E. (2002): Nitric oxide in acute renal failure: NOS versus NOS. Kidney Int., (3): 855-61.
Guo, Q.; Zahao, B.; Shen, S.; Hou, J.; Hu, J. and Xin, W. (1999): ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers. Biochimica et Biophysica Acta, 1427: 13-23.
Habig, W.H.; Pabst, M.J. and Jakoby, W.B. (1974): Glutathione- S-transferases. The first enzymatic step in mercaptouric acid formation. Journal of biological chemistry, 249: 7130-7139.
Hanaa, A.; Sherif, M.; Rajiv, L.; Augusta, F.; Madhwa, H. and Allal, O. (2009): In vivo evidence of hepato- and reno-protective effect of garlic oil against sodium nitrite-induced oxidative stress. International Journal ofBiological Sciences, 5 (3): 249-255.
Hassan, H.A.; El-Agamy, S.M.; Gaur, R.; Fernando, A.; Raj, M.H. and Quhtit, A. (2009): In vivo evidence of hepato and reno-protective effect of garlic oil against sodium nitrite induced oxidative stress. International Journal of Biological Science, 5(3): 249-255.
Henry, R.J. (1974): Principles and Techniques. Clinical Chemistry, 2nd ed. Harper and Row, 525.
Higdon, J.V. and Frei, B. (2003): Tea catechins and polyphenols: health effects metabolism and antioxidant functions. Critical Reviews in Food Science and Nutrition, 43 (1): 89–143.
Hill, M. (1991): Nitrates and nitrites in food and water. Ellis Horwood, New York, (pp. 131-132).
Hockenberg, D.; Nunez, G.; Milliman, S.; Schreiber, R.D. and Korsmeyer, S.J. (1990): Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature, 348 (9299): 334-336.
Ilntisky, A.P. and Kolpakova, A.S. (1997): The enhancing effect of sodium nitrite on virus-induced leukemia in mice. Cancer Detection Prevention, 21: 312.
Jahries, G.; Hesse, V.I.; Schone, L.H. and Mehnert, E. (1986): Influence of nitrates and plant goitrgens on thyroid hormone, somated in status and growth of swine. JournalofVeterinary Medicine, 41: 528-530.
Kim, H.J.; Chang, W.K.; Kim, M.K.; Lee S.S. and Choi, B.Y. (2002): Dietary factors and gastric cancer in Korea: Case-control study. International Journal of Cancer, 97: 531-535.
Kirshnamoorthy, P. and Sangeetha, M. (2008): Protective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats. Indian Journal of Biochemistry and Biophysics, 45: 206-208.
Koracevic, G.; Djordjevic,V.; Andrejevic, S. and Cosic, V. (2001): Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology, 54: 356-361.
Lorenz, M.; Wessler, S. and Follmann, E. (2004): A constituent of green tea, epigallocatechin-3-gallate, activates endothelial nitric oxide synthase by a phosphatidylinositol-3-OH-kinase-, cAMP-dependent protein kinase-, and Akt-dependent pathway and leads to endothelial-dependent vasorelaxation. J. Biol. Chem. (13): 6190-6195.
Lung, H.L.; Ip, W.K.; Wong, C.K.; Mak, N.K.; Chen, Z.Y. and Leung, K.N. (2002): Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line. Life Science, 72 (3): 257–268.
Mcknight, G.M.; Smith, L.M.; Drummond, R.S.; Duncan, C.W.; Golden, M. and Benjamin, N. (1997):Chemical synthesis of nitric oxide in the stomach from dietary nitrate in humans. Gut, 40: 212-214
Mohamadin, A.; El-Beshbishy, H. and El-Mahdy, M. (2005): Green tea extract attenuates cyclosporine A-induced oxidative stress in rats. Pharmacological Research, 51: 51-57.
Montgomery, H.A.C. and Dymock, J.F. (1961): Determination of nitric oxide. Analyst, 86: 414.
National Toxicology Program, (2001): Toxicology and carcinogenesis studies of sodium nitrite CAS No. 7632-00-0 in F344/N rats and B6C3F1 mice drinking water studies. Nat. Toxicol. Program Tech. Rep. Ser., 495: 7-273.
Ohkawa, H.; Ohishi, N. andYagi, K. (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 95: 351–358.
Oliver, I.T.A. (1955): Spectrophotometric method for the determination of creatine phosphokinase and myokinase. Biochemical Journal, 61:116-122.
Ostrowska, J.; Luczaj, W.; Kasacka, I; Rózanski, A. and Skrzydlewska, E. (2004): Green tea protects against ethanolinduced lipid peroxidation in rat organs. Alcohol, 32: 25-32.
Özer, A.B.; Kaman, D.; Erhan, Ö.L. and Özer, S. (2008): EGCg supplementation improves oxidant and antioxidant status in kidney of rats exposed to sevoflurane. Fırat. Tıp. Derg., 13: 1.
Peters, J.M.; Preston-Martin, S.; London, S.J.; Bowman, J.D.; Buckley, J.D. and Thomas, D.C. (1994): Processed meats and risk of childhood leukemia. Cancer Causes and Control, 5: 195-202.
Prasad, J. (1983): Effect of high nitrate diet on thyroid glands in goats. Indian Journal of Animal Sciences, 53: 791-794.
Raabo, E. (1963): Determination of serum lactic dehydrogenase by the tetrazolium salt method. Scandinavian Journal of Clinical and Laboratory Investigation, 15: 233-238.
Rees, D.D.; Cellek, S.; Palmer, R.M. and Moncada, S. (1990): Dexamethasone perevents the induction by indotoxin of a nitric oxide synthase and the associated effects on vascular one: an insight into endotoxine shock. Biochem. Biophys. Res. Commun., 173: 541-547.
Reisser, D.; Lagadec, P.; Arnould, L.; Arnould, N.; Maupoil, V.; Pinard D. and Eannin, J.F. (1998): Nitric oxide inhibits proliferation but increases life-span of Tlymphocytesin tumor-bearing rats. Cancer Immunology, Immunotherapy, 46: 160-166.
Reitman, S. and Frankel S. (1957): A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American Journal of Clinical Pathology, 28: 56-63.
Relja, B.; Tottel, E.; Bteig, L.; Henrich, D.; Schneider, H.; Marzi, I. and Lehnert, M. (2010): Effects of green tea catechins on the pro-inflammatory response after haemorrhage/resuscitation in rats. Br. J. Nutr., 4: 1-7.
Sanchez-Echaniz, J.; Benito-Frnandez, J. and Mintegui-Raso, S. (2001): Methemoglobinemia and consumption of vegetables in infants. Pediatrics, 107: 1024-1028.
Sano, M.; Takahashi, Y.; Yoshino, K.; Shimoi, K.; Nakamura, Y.; Tomita, I.; Oguni, I.; and Konomoto, H. (1995): Effect of tea (Camellia sinensis L.) on lipid peroxidation in rat liver and kidney: a comparison of green and black tea feeding. Biological and Pharmaceutical Bulletin, 18 (7): 1006–1008.
Sarsour, A. and Hassuneh, N.E. (2001): The effect of sodium nitrite on some parameters of the immune system. Food Chem Toxicol., 39 (2): 119-24.
SAS (2001): Statistical Analysis System. Users Guide: statistics, (SAS Institute, CaryNorth Carolina).
Schroeder, P.; Klotz, L.O. and Sies, H. (2003): Amphiphilic properties of (−) epicatechin and their significance of protection of cells against peroxynitrite. Biochemical and Biophysical Research Communications, 307 (1): 69–73.
Shahjahan, M.; Vani, G. and Shyamaladevi, C.S. (2005): Effect of Solanum trilobatum on the antioxidant status during diethyl nitrosamine induced and phenobarbital promoted hepatocarcinogenesis in rat. Chemo- Biological Interaction, 156: 113-123.
Skryzdlewska, E.; Ostrowska, J.; Stankiewicz, A. and Fabisszewski, R. (2002): Green tea as a potent antioxidant in alcohol intoxication. Addiction Biology, 7: 307-314.
Sleight, S.D.; Sinha, D.P. and Uzoukwu, M. (1972): Effect of sodium nitrate on reproductive performance of pregnant sows. Journal of the American Veterinary Medical Association, 161: 819-823.
Swann, P.F. (1975): The toxicology of nitrate, nitrite and N-nitroso compounds. Journalof theScienceofFoodandAgriculture, 26: 1761-1770.
Szemes, Z. and Szamado, I. (1991): Comparasion of histological effect of electrocoagulation and Nd-YAG laser coagulation in intact and timorous rat tissue. Acta Chir. Hung., 32: 245-251.
Teppema, L. J.; Nieuwenhuijs, D.; Sarton, E.; Romberg, R.; Wards, D.S. and Dahan, A. (2002): The Journal of Physiology, 549: 931-938.
Tietz, N.W. (1990): Clinical guide to laboratory tests. 2nd ed. Philadelphia:WB Saunders, 26-29.
Wan, Y.; Vinson, J.; Etherton, T.; Porch, J.; Lazarus, S. and Kris-Etherton, P. (2001): Effects of cocoa powder and dark chocolate on LDL oxidative susceptibility and prostaglandin concentration in humans. American Society for Clinical Nutrition, 74: 596-602.
Wink, D.A.; Kasprzak, K.S.; Maragos, C.M.; Elepuru, R.K.; Misra, M.; Dunams, T.M.; Cebala, A.; Koch, W.H.; Andrews, A.W.; Allen, J.S. and Keefer, L.k. (1991): DNA deaminating ability and genotoxicity of nitric oxide and its progenitors. Science, 254: 1001- 1003.
World Health Organization, (2003): Safety evaluation of certain food additives. Fifty-fourth Report of the Joint FAO/WHO Committee on Food Additives. Food Additives Series No. 50. Genera: WHO.
Wu, G.; Fang, Y.Z.; Yang, S.; Lupton, J.R. and Turner, N.D. (2004): Glutathione metabolism and its implications for health. Journal of Nutrition, 134: 489-492.
Zaki, A.; Chaoui, A.A.; Chait, A.; Aboussaouira, T.; Zarrouk, K. and Himmi, T. (2005): Effect of inorganic nitrates on the morphofunctional condition of the kidney in the rat. Therapie., 60(1): 75-9.
Zurovsky, Y. and Haber, C. (1995): Antioxidants attenuate endotoxin- generation induced acute renal failure in rats. Scandinavian Journal of Urology and Nephrology, 29: 147-154.
Xu, C.; Qunshu, W.; Qun Qiu; Zhao, Q. and Cao, J. (2007): Protective effects of green tea polyphenols against subacute epatotoxicity induced by microcystin-LR in mice. Environmental Toxicology and Pharmacology 24: 140–148. | |||||||||||||||||||||
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