Venom Composition of Egyptian and Carniolan Honeybee, Apis mellifera L. Affected by Collection Methods. | ||||
Egyptian Academic Journal of Biological Sciences. A, Entomology | ||||
Article 6, Volume 11, Issue 4, August 2018, Page 59-71 PDF (566.53 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsa.2018.17733 | ||||
View on SCiNiTO | ||||
Authors | ||||
Hind Abd El- Ghany Zidan1; Zahia K. Mostafa2; Mahmoud A. Ibrahim3; S. I. Haggag1; Doaa A. Darwish3; Abir A. Elfiky4 | ||||
1Plant Protection Research Institute, Agriculture Research Centre | ||||
2Entomology Department, Faculty of Science, Ain Shams University | ||||
3Molecular Biology Department, National Research Centre, El-Tahrir st., Dokki, Giza, Egypt | ||||
4Director of ANDI center of excellence in antivenom research, Vacsera, Egypt | ||||
Abstract | ||||
Bee venom is one of the economic income sources for beekeepers due to their widely medical uses and other traded products. This study investigates the biological activity of venom extracts from the honeybee, Apis mellifera subspecies: A. m. lamarckii and A. m. carnica by manual and electro- stimulated extracted methods. Venoms extracted compared with regard to their SDS-PAGE analysis, enzymatic activities: protease, PLA2, hyaluronidase and haemolytic activity of melittin, and LD50 value. The electrophoretic analysis data showed that manual venoms extract for Egyptian and Carniolan bees contain 24 and 21 bands with molecular weights ranging from 127.66 - 15.92 KDa and 133.61- 14.4 KDa, respectively. It was noted that the venoms electrically extracted for Egyptian and Carniolan bees have the highest similarities with 0.363. The enzymatic activities and LD50 value data showed that the venoms extracted using electrical stimulated method has high optimal quality and it can be commercialized by the beekeeper. The venom of Egyptian subspecies A. m. lamarckii has high biological activity and is the better form for the best pharmacological source. The experimental data can be used for identification, standardization and determining the biological activity of honeybee venom in Egypt. | ||||
Keywords | ||||
honeybee subspecies/ bee venom/ collection methods / SDS-PAGE / enzyme activities / LD50 | ||||
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