Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates
Sakr, M., Ibrahim, N., Ali, S., Alzahaby, N., Omar, A., Khairy, W., Zohdy, B., Qassem, O., Saleh, S. (2021). Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates. EKB Journal Management System, 5(1), 128-142. doi: 10.21608/aps.2021.76856.1062
Masarra Sakr; Nehal Ibrahim; Sandy Ali; Nouran Alzahaby; Aya Omar; Walaa Khairy; Bassant Zohdy; Omar Qassem; Sarra Saleh. "Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates". EKB Journal Management System, 5, 1, 2021, 128-142. doi: 10.21608/aps.2021.76856.1062
Sakr, M., Ibrahim, N., Ali, S., Alzahaby, N., Omar, A., Khairy, W., Zohdy, B., Qassem, O., Saleh, S. (2021). 'Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates', EKB Journal Management System, 5(1), pp. 128-142. doi: 10.21608/aps.2021.76856.1062
Sakr, M., Ibrahim, N., Ali, S., Alzahaby, N., Omar, A., Khairy, W., Zohdy, B., Qassem, O., Saleh, S. Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates. EKB Journal Management System, 2021; 5(1): 128-142. doi: 10.21608/aps.2021.76856.1062

Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates

Archives of Pharmaceutical Sciences Ain Shams University
Article 10, Volume 5, Issue 1, June 2021, Page 128-142  XML PDF (904.15 K)
Document Type: Original Article
DOI: 10.21608/aps.2021.76856.1062
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Authors
Masarra Sakr email orcid 1; Nehal Ibrahimorcid 2; Sandy Ali3; Nouran Alzahaby3; Aya Omar3; Walaa Khairy3; Bassant Zohdy3; Omar Qassem4; Sarra Salehorcid 1
1Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
2Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
3Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
Abstract
Over the last decades, the development of microbial resistance has become an alarming situation. This has urged the search for new antimicrobial strategies. In this context, two Brassicaceae edible plants; Brassica oleracea var. capitata (cabbage) and Brassica rapa subsp. rapa (Turnip) were assessed for their antimicrobial activity against P. aeruginosa and E. coli clinical isolates. Antibiogram analysis was done according to the CLSI 2019 guidelines and proved that both P. aeruginosa and E. coli clinical isolates were multidrug resistant. A green extraction methodology – assisted by microwave and ultrasound- was used to prepare the aqueous extracts. Determination of minimum inhibitory concentration (MIC) of the extracts was also carried out according to the CLSI guidelines. At sub-MIC concentration, cabbage extract showed promising results in the inhibition of quorum sensing mediated virulence determinants of P. aeruginosa. Highest reduction was observed in pyocyanin and rhamnolipid production. Chemical profiling via UPLC-ESI-MS analysis of cabbage extract revealed the presence of different glucosinolates together with iberin and sulforaphane. The in silico docking study was conducted and revealed the ability of sulforaphane and iberin to bind to LasR regulator responsible for quorum sensing in P. aeruginosa. These compounds thus represent potential candidates that can be developed into novel antimicrobial infection control tools.
Keywords
Quorum sensing; Iberin; Sulforaphane; Cabbage; Resistance; Docking
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