Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation. | ||||
The Egyptian Journal of Hospital Medicine | ||||
Article 2, Volume 26, Issue 1, January 2007, Page 22-30 PDF (794.64 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejhm.2007.17777 | ||||
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Authors | ||||
Hanafi N1; Abel Baset El Aaser2; Mohamed S. Gabry3; Amany A Tohamy3; Manal El Saied Ebrahim3 | ||||
1Radiation Biology Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt | ||||
2cancer Biology Depart. Cancer Institute, Cairo University | ||||
3Zoology Dept. Faculty of Science, Helwan University. | ||||
Abstract | ||||
Introduction: Although there is little doubt that antioxidants are a necessary component for good health, no one knows if supplements should be taken and, if so, how much. Materials and methods: To investigate the increase in antioxidants supplementation, a group of mice feeding on a diet containing 20% soybean was used as control group. The control group treated with 5% Nigella sativa and Antox drug (3 mg/mouse/day) either alone or in combination for 15 days. Another control group was treated with the same antioxidants and subjected to 1 Gy of γ- irradiation x 5 times day after day. In kidney tissue the levels of malonaldehyde (MDA) and reduced glutathione (GSH) were estimated. Also, the histopathological changes were recorded. Results: 5% Nigella sativa revealed a little decrease in MDA level and a highly significant decrease in GSH in kidney tissue while the exposure to fractionated dose of γ- irradiation predicted a highly significant decrease in MDA and a highly significant increase in GSH level. Gastric intubations of Antox drug showed a highly significant increase in MDA and GSH levels in kidney tissue either alone or in addition to fractionated radiation exposure. Combined treatments of Nigella sativa and Antox drug showed a highly significant decrease in MDA level and a highly significant increase in GSH level in kidney tissue in comparison to the control group. Histopathological changes showed that treatment with 5% Nigella sativa and Antox drug either alone or in combination revealed toxic effect on kidney tissue. However, their treatments prior to radiation exposure showed ameliorating effect. Conclusion: Our findings indicated that we under oxidative stress. | ||||
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