Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.
N, H., El Aaser, A., Gabry, M., Tohamy, A., Ebrahim, M. (2007). Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.. EKB Journal Management System, 26(1), 22-30. doi: 10.21608/ejhm.2007.17777
Hanafi N; Abel Baset El Aaser; Mohamed S. Gabry; Amany A Tohamy; Manal El Saied Ebrahim. "Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.". EKB Journal Management System, 26, 1, 2007, 22-30. doi: 10.21608/ejhm.2007.17777
N, H., El Aaser, A., Gabry, M., Tohamy, A., Ebrahim, M. (2007). 'Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.', EKB Journal Management System, 26(1), pp. 22-30. doi: 10.21608/ejhm.2007.17777
N, H., El Aaser, A., Gabry, M., Tohamy, A., Ebrahim, M. Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.. EKB Journal Management System, 2007; 26(1): 22-30. doi: 10.21608/ejhm.2007.17777

Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.

The Egyptian Journal of Hospital Medicine
Article 2, Volume 26, Issue 1, January 2007, Page 22-30  XML PDF (794.64 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2007.17777
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Authors
Hanafi N1; Abel Baset El Aaser2; Mohamed S. Gabry3; Amany A Tohamy3; Manal El Saied Ebrahim3
1Radiation Biology Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
2cancer Biology Depart. Cancer Institute, Cairo University
3Zoology Dept. Faculty of Science, Helwan University.
Abstract
Introduction: Although there is little doubt that antioxidants are a necessary component for good health, no one knows if supplements should be taken and, if so, how much.
Materials and methods: To investigate the increase in antioxidants supplementation, a group of mice feeding on a diet containing 20% soybean was used as control group. The control group treated with 5% Nigella sativa and Antox drug (3 mg/mouse/day) either alone or in combination for 15 days. Another control group was treated with the same antioxidants and subjected to 1 Gy of γ- irradiation x 5 times day after day. In kidney tissue the levels of malonaldehyde (MDA) and reduced glutathione (GSH) were estimated. Also, the histopathological changes were recorded.
Results: 5% Nigella sativa revealed a little decrease in MDA level and a highly significant decrease in GSH in kidney tissue while the exposure to fractionated dose of γ- irradiation predicted a highly significant decrease in MDA and a highly significant increase in GSH level. Gastric intubations of Antox drug showed a highly significant increase in MDA and GSH levels in kidney tissue either alone or in addition to fractionated radiation exposure. Combined treatments of Nigella sativa and Antox drug showed a highly significant decrease in MDA level and a highly significant increase in GSH level in kidney tissue in comparison to the control group. Histopathological changes showed that treatment with 5% Nigella sativa and Antox drug either alone or in combination revealed toxic effect on kidney tissue. However, their treatments prior to radiation exposure showed ameliorating effect.




Conclusion: Our findings indicated that we under oxidative stress.
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