DMBA and Lagenaria Siceraria Biological Effects: 1: Is it Possible for Lagenaria Siceraria to be a Treatment for The Carcinogenesis in The Ileum of DMBA-Induced Male Swiss Albino Mice
Aref, A., Semmler, M., Jad, M., Mohamadain, H. (2020). DMBA and Lagenaria Siceraria Biological Effects: 1: Is it Possible for Lagenaria Siceraria to be a Treatment for The Carcinogenesis in The Ileum of DMBA-Induced Male Swiss Albino Mice. EKB Journal Management System, 12(2), 59-75. doi: 10.21608/eajbsd.2020.177829
Abdel-Baset M. Aref; Margit Semmler; Mariam M. Jad; Hoda Mohamadain. "DMBA and Lagenaria Siceraria Biological Effects: 1: Is it Possible for Lagenaria Siceraria to be a Treatment for The Carcinogenesis in The Ileum of DMBA-Induced Male Swiss Albino Mice". EKB Journal Management System, 12, 2, 2020, 59-75. doi: 10.21608/eajbsd.2020.177829
Aref, A., Semmler, M., Jad, M., Mohamadain, H. (2020). 'DMBA and Lagenaria Siceraria Biological Effects: 1: Is it Possible for Lagenaria Siceraria to be a Treatment for The Carcinogenesis in The Ileum of DMBA-Induced Male Swiss Albino Mice', EKB Journal Management System, 12(2), pp. 59-75. doi: 10.21608/eajbsd.2020.177829
Aref, A., Semmler, M., Jad, M., Mohamadain, H. DMBA and Lagenaria Siceraria Biological Effects: 1: Is it Possible for Lagenaria Siceraria to be a Treatment for The Carcinogenesis in The Ileum of DMBA-Induced Male Swiss Albino Mice. EKB Journal Management System, 2020; 12(2): 59-75. doi: 10.21608/eajbsd.2020.177829

DMBA and Lagenaria Siceraria Biological Effects: 1: Is it Possible for Lagenaria Siceraria to be a Treatment for The Carcinogenesis in The Ileum of DMBA-Induced Male Swiss Albino Mice

Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry
Article 5, Volume 12, Issue 2, December 2020, Page 59-75  XML PDF (1.84 MB)
Document Type: Original Article
DOI: 10.21608/eajbsd.2020.177829
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Authors
Abdel-Baset M. Aref1; Margit Semmler2; Mariam M. Jad3; Hoda Mohamadain4
1-Cell Biology and Histochemistry Division, Zoology Department, Faculty of Science, South Valley University, Qena, Egypt. -Vice President of Institutional Animal Cure and Use Committee of South Valley University in Egypt (IACUC-SVU-Egypt).
2Diabetes Research Institute, Düsseldorf University, Düsseldorf, Germany. IACUC of SVU in Egypt
3Cell Biology & Histochemistry Division, Zoology Department, Faculty of Science, South Valley University, Qena, 83523, Egypt. IACUC of SVU in Egypt.
4Parasitology Division, Zoology Department, Faculty of Science, South Valley University, Qena, 83523, Egypt. IACUC of SVU in Egypt.
Abstract
Nowadays, tumors are recorded a high percentage among human beings due to exposure to different carcinogens. DMBA and mouse will be established models for study tumorigeneses.  A trial was done to suppress cell proliferation using Lagenaria siceraria (LS). Mice were divided into C: control animals received a single injection of corn oil and a daily dose of distilled water, D: animals received a single dose of DMBA and 91 days ago, mice received morning and night daily oral dose of distilled water for 30 days, and DL: Animals received a single dose of DMBA (10 mg/ 100 g b.w.) and 91 days ago, mice received morning and night daily oral dose of LS (100 mg/ Mouse) for 30 days. Our results showed a single dose of DMBA (10 mg/ 100 g b.w.) has a stimulatory effect on the cellular activity, DNA synthesis, and cell proliferation of epithelial cells in the ileum, however, LS mostly treated this effect. BAX and BCL2 immunohistochemistry revealed DMBA induced excessive cell proliferation and hyperplasia (dysplasia), while LS almost treated this effect of DMBA. These results suggest LS modulates the toxic effect induced by DMBA in the ileum. A promising data for cancer herbal therapy in human beings.
Keywords
DMBA; Lagenaria siceraria; Cell proliferation; Tumor, Cell Biology; Histochemistry; Immunohistochemistry; Histopathology
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