Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar
HEGAZI, E., Yousef, A., ABD ALLATIF, A., . Mahmoud, T., Mostafa, M. (2021). Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar. EKB Journal Management System, 64(12), 6961-6969. doi: 10.21608/ejchem.2021.78774.3853
EL SAID SADEK HEGAZI; Aml Yousef; ABDOU MOHAMED ABD ALLATIF; Thanaa Shaban. Mohamed . Mahmoud; Mona Kamal Mohamed Mostafa. "Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar". EKB Journal Management System, 64, 12, 2021, 6961-6969. doi: 10.21608/ejchem.2021.78774.3853
HEGAZI, E., Yousef, A., ABD ALLATIF, A., . Mahmoud, T., Mostafa, M. (2021). 'Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar', EKB Journal Management System, 64(12), pp. 6961-6969. doi: 10.21608/ejchem.2021.78774.3853
HEGAZI, E., Yousef, A., ABD ALLATIF, A., . Mahmoud, T., Mostafa, M. Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar. EKB Journal Management System, 2021; 64(12): 6961-6969. doi: 10.21608/ejchem.2021.78774.3853

Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

Egyptian Journal of Chemistry
Article 8, Volume 64, Issue 12, December 2021, Page 6961-6969  XML PDF (365.09 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.78774.3853
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Authors
EL SAID SADEK HEGAZIorcid 1; Aml Yousef email orcid 2; ABDOU MOHAMED ABD ALLATIForcid 3; Thanaa Shaban. Mohamed . Mahmoudorcid 4; Mona Kamal Mohamed Mostafa5
1, Faculty of Agricultural., Cairo University
2horticultural crops technology-NRC.EGYPET
3Faculty of Agricultural., Cairo University
4Horticultural Crops Technology Department, National Research Centre, 33 El-Bohouth St.,
5Horticultural Crops Technology Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza, Egypt
Abstract
Micro-propagation of olive provides the possibility of producing clones of native plants with appropriate attributes and mass production in short period of time, compared to conventional methods. Micropropagation of olive requires optimization of protocols and adaptation to specific cultivars requirements. This experiment was carried out during 2020 and 2021 seasons in tissue culture laboratory of Pomology Department, Faculty of Agriculture, Cairo University in order to evaluate the potential effects of nano silver at (0, 5, 10 and 20 mg L-1) to improve sprouting percentage and shoot growth of Picual olive cv. The effect of nutrient media composition (MS modified, OM and OM modified) and cytokinins (BAP and Zeatin) on multiplication rate and shoot growth were examined. IBA and NAA (2 and 4 mg L) was examined during rooting stage. The obtained results cleared that Silver Nanoparticles (AgNPs) at 5 mg L-1 recorded the highest percentage of bud sprouting, shoot length, number of shoot/explant and number of leaves/ shoot. Olive medium (OM) recorded the highest shoot length, number of shoot/explant and number of leaves/ shoot compared with OM modified and MS modified media. Also, Zeatin at 4 mg L-1 recorded the highest shoot growth parameters during in vitro multiplication stage. During in vitro rooting stage, both the type and concentration of auxins have a significant influence on number of roots, root length and rooting % of Picual olive cultivar. In conclusion, it is recommended that very low concentrations of nano-silver particles be used in plants tissue culture on olive media (OM) supplemented with Zeatin at 4mgL-1.
Keywords
Nano-silver particles; disinfection; in vitro micropropagation; media composition and olive tree
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