Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83

Elsayed, Noha S.; Aboshanab, Khaled M.; Yassien, Mahmoud A.; Aboulwafa, Mohammad M.; Hassouna, Nadia A.

doi:10.21608/aps.2021.76831.1061

	Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83
Archives of Pharmaceutical Sciences Ain Shams University
Article 12, Volume 5, Issue 1, June 2021, Page 158-170 PDF (773.42 K)
Document Type: Original Article
DOI: 10.21608/aps.2021.76831.1061
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Authors
Noha S. Elsayed ¹; Khaled M. Aboshanab ¹; Mahmoud A. Yassien¹; Mohammad M. Aboulwafa²; Nadia A. Hassouna¹
¹Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
²1. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University 2. Faculty of Pharmacy, King Salman International University, Ras-Sedr, South Sinai, Egypt
Abstract
Background: Polyhydroxyalkanoates (PHA) are environmentally friendly polymers produced by many bacteria under nutrient-limited conditions. However, their commercialization is hindered by the production expenses. The present study aimed at cost-effective and efficient production of poly(3-hydroxybutyrate) (PHB) by Bacillus (B.) cereus isolate CCASU-P83. Methods: Through one factor at time optimization study on shake flask, B. cereus CCASU-P83 produced about 50 % polymer per dry weight after 48 hours incubation time. For better evaluation of the fermentation process, kinetic modeling using the Logistic and Leudking-piret models was applied. Results: A preliminary economic analysis was carried out and leads to a 30.8 % reduction in the total cost. In comparison to the findings obtained on the level of shake flask, scaling up to the bioreactor resulted in producing about 53% PHB per dry weight after only 24 hours incubation. These models concluded that B. cereus produced PHB during the growth phase. Analysis of molecular weight of the produced polymer displayed a 26900 g/mole molecular weight with a polydispersity index (PDI) of 1.1. Conclusion: B. cereus CCASU-P83 is a potential candidate for industrial production of PHB polymer using corn oil in a short incubation period which highly reduced the cost of the production process.
Keywords
Polyhydroxyalkanoates; Logistic; Leudking; Fermentation; optimization


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