Disposition Kinetics of Cefepime in Healthy and Experimentally Salmonella Typhimurium Infected Broiler Chicken | ||||
| SVU-International Journal of Veterinary Sciences | ||||
| Article 6, Volume 1, Issue 1, June 2018, Page 66-81 PDF (714.97 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/svu.2018.17931 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Taha Atti1; Hanan Azoz* 2; Mohamed Shamakh2 | ||||
| 1Department of Pharmacology, Faculty of Veterinary Medicine, University of Sadat City, | ||||
| 2Biochemistry, Toxicology and Feed Deficiency Department “Pharmacology Unit”, Animal Health Research Institute, Dokki, Egypt. | ||||
| Abstract | ||||
| Cefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentallySalmonella typhimuriuminfected broiler chickens were determined.Cefepime concentration in serumsamples was determined by reverse-phase high performance liquid chromatography with mobile phase. The mobile phase was a mixture of 10mM phosphate buffer (pH 7): Methanol; 75:25 was always freshly prepared. Flow rates were 1 ml/min. UV detection was performed at 256 nm, injection volume was 20 µl. After a single intravenous injection, cefepime reached its maximum serum concentrations of 4.28 ± 0.37μg/mlin normalchickens, while in the infected chickens, the maximum serum concentration was 2.62 ± 0.72 μg/ml. Cefepime was eliminated after intravenous injection with half-life (t1/2 β) of 4.608 ± 0.145 h in normal which significantly longer than 4.19 ± 0.158 h in infected chickens. The mean residence time (MRT) was 6.51 ± 0.189h in normal vs5.86±0.18 h in infected chickens. After IMadministration the drug reached its maximum serum concentrations of 193.06 ± 2.27μg/ml at maximum time of 1.138 ± 0.012 h in normal, while in infected chickens the maximum serum concentrations was 132.93 ± 1.53μg/mlattained atmaximum timeof 1.265 ± 0.013 h. In conclusion a cefepime at dose of 100 mg/kg administered intravenously or IMat 24 h intervals may provide successful treatment of chicken infected with Salmonellatyphimurium | ||||
| Keywords | ||||
| Cefepime; Salmonella typhimurium; High performance liquid chromatography; broiler; chicken | ||||
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