Antiproliferative and antioxidant activities of the edible crab Callinectes sapidus hepatopancreas and hemolymph extracts
M. Zakzok et al., S. (2021). Antiproliferative and antioxidant activities of the edible crab Callinectes sapidus hepatopancreas and hemolymph extracts. EKB Journal Management System, 25(3), 531-550. doi: 10.21608/ejabf.2021.179659
Somaia M. Zakzok et al.. "Antiproliferative and antioxidant activities of the edible crab Callinectes sapidus hepatopancreas and hemolymph extracts". EKB Journal Management System, 25, 3, 2021, 531-550. doi: 10.21608/ejabf.2021.179659
M. Zakzok et al., S. (2021). 'Antiproliferative and antioxidant activities of the edible crab Callinectes sapidus hepatopancreas and hemolymph extracts', EKB Journal Management System, 25(3), pp. 531-550. doi: 10.21608/ejabf.2021.179659
M. Zakzok et al., S. Antiproliferative and antioxidant activities of the edible crab Callinectes sapidus hepatopancreas and hemolymph extracts. EKB Journal Management System, 2021; 25(3): 531-550. doi: 10.21608/ejabf.2021.179659

Antiproliferative and antioxidant activities of the edible crab Callinectes sapidus hepatopancreas and hemolymph extracts

Egyptian Journal of Aquatic Biology and Fisheries
Article 36, Volume 25, Issue 3, May and June 2021, Page 531-550  XML PDF (878.13 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2021.179659
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Author
Somaia M. Zakzok et al.
Abstract
Crabs, decapods crustaceans, have been recognized as a rich natural source of unique antioxidant and therapeutic agents. Callinectes sapidus is an edible crab used as a popular food source in coastal cities like Port Said, Egypt. In the present study, in vitro  cytotoxic and antioxidant properties of the hepatopancreas and hemolymph of C. sapidus were investigated on some human cell lines (HeLa, HepG 2, MCF-7,  MDA-MB-231 and  WI-38). The MTT results showed that both extracts possess selective cytotoxicity against HepG2, MCF-7 and HeLa. No cytotoxicity was observed on normal lung cells (WI-38). The hepatopancreas and hemolymph showed the highest antiproliferative activity against HeLa cells (IC50: 0.39 and 2.01 mg/ml respectively). Flow cytometry revealed that both extracts can induce apoptosis in HeLa cells and cause cell cycle arrest in the G2/M phase after 48 h treatment.  Hepatopancreas showed greater DPPH radical-scavenging activity with IC50 value of 38.99 µg/ml than hemolymph (IC50 value of 464 µg/ml). Both extracts at 2 mg/ml were effective to attenuate the perturbations in the tested parameters (superoxide dismutase, catalase, reduced glutathione and malondialdehyde) of H2O2-induced oxidative stress in WI-38 cells. Findings suggest that these blue crab extracts have protective effects on H2O2-induced lipid peroxidation and might be used for the development of new drugs against human cervical adenocarcinoma.
Keywords
Blue crabs; Hepatopancreas; Hemolymph; Antiproliferative; Apoptosis; Antioxidant
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