Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt
Abd-El-Maogoud, H., Edris, A., Mahmoud, A., Maky, M. (2021). Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt. EKB Journal Management System, 4(2), 76-84. doi: 10.21608/svu.2021.64991.1111
Hadeer Abd-El-Naser Abd-El-Maogoud; Abo Baker Moustafa Edris; Ayman Hammed Mahmoud; Mohamed Abdelfattah Maky. "Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt". EKB Journal Management System, 4, 2, 2021, 76-84. doi: 10.21608/svu.2021.64991.1111
Abd-El-Maogoud, H., Edris, A., Mahmoud, A., Maky, M. (2021). 'Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt', EKB Journal Management System, 4(2), pp. 76-84. doi: 10.21608/svu.2021.64991.1111
Abd-El-Maogoud, H., Edris, A., Mahmoud, A., Maky, M. Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt. EKB Journal Management System, 2021; 4(2): 76-84. doi: 10.21608/svu.2021.64991.1111

Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt

SVU-International Journal of Veterinary Sciences
Article 7, Volume 4, Issue 2 - Serial Number 8, June 2021, Page 76-84  XML PDF (308.16 K)
Document Type: Research article
DOI: 10.21608/svu.2021.64991.1111
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Authors
Hadeer Abd-El-Naser Abd-El-Maogoud1; Abo Baker Moustafa Edris2; Ayman Hammed Mahmoud1; Mohamed Abdelfattah Maky email orcid 3
1Biotechnology and Food Control Department, Animal Health Institute, Doki ,Giza, Egypt.
2Food Control Department, Faculty of Vet Medicine, Benha, 13511, Egypt
3Food Hygiene Department, Faculty of Veterinary Medicine - South Valley University
Abstract
The aims of this study were isolation and characterization of Pseudomonas spp. from fish meat collected from Sohag governorate, Egypt. A total 120 fish samples including frozen mackerel, frozen saurus, chilled Mugil cephalus and chilled Tilapia nilotica (30 of each) were collected from different shops and super markets in Sohag governorate. Pseudomonas spp. were isolated from 65% of examined samples. The obtained data revealed that the highest count of Pseudomonas was in chilled Tilapia nilotica. The prevalence of Pseudomonas aeruginosa in frozen mackerel, frozen saurus, chilled Tilapia nilotica and chilled Mugil cephalus was 33.3%, 30%, 23.3% and 26.6% respectively. Furthermore, psychrotrophic count was performed and the results demonstrated that it was the highest in frozen mackerel followed by Tilapia nilotica and the Mugil cephalus showed the lowest count. Furthermore, the occurrence of oprL, phzM and toxA virulence genes was studied in some selected isolates by PCR. The findings showed that all the selected isolates possessed the virulence genes. This work showed contamination of fish samples with Pseudomonas spp., indicating the importance of applying of hygienic measures during handling and storage of fish.
Keywords
Pseudomonas aeruginosa; contamination; PCR; virulence genes
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