Studying the Risks of Cotton Cooking Oil Fumes on the Lung and Liver of Rats and the Protective Role of (Vitamin E Alphatocopherol ) | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 5, Volume 21, Issue 1, October 2005, Page 53-65 PDF (433.53 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2005.18049 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Fahmy GAD GAD ELsaid | ||||
| Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt. | ||||
| Abstract | ||||
| Fumes emitted from edible vegetable cooking oils during stir- and deep-frying are important contributors to indoor air pollution. Indoor air pollution may be increase the lung and liver cancer in Egypt in 2005. Therefore, the present study was undertaken to investigate the effects of Egyptian cotton cooking oil fumes on rats. The exposed rats for 30 and 60 days to cotton oil fumes showed a significant increase in the lung and liver malondialdehyde levels which accompanied with a significant decrease in glutathione content. Also, there was a significant decrease in lung and liver superoxide dismutase, catalase, glutathione s-transferase activities. As well as, there was a significant decrease in serum amino acids levels, lung and liver nucleic acids and total proteins. These changes were obviously after 60 days than that of 30 days of exposure. DNA change was clear in the lungs of rats after cotton oil fumes exposed as showed by the differential display technique, P53 primer which used to study the expression of the p35 gene as well as to confirm and amplify these changes after oil fumes exposure. Band with different molecular weights were observed after the exposure and in protected groups but not presented in the control. More characterization for the changes had been carried out in the animal on two levels, one the DNA using RAPD-PCR and the other on the protein level using SDS-PAGE techniques. Also, vitamin E ameliorates these abnormalities to extent limit in all cases in this work. | ||||
| Keywords | ||||
| cooking oil fumes; cotton oil; Polycyclic aromatic hydrocarbons (PAHs); superoxide dismutase (SOD); catalase (CAT); Glutathione S-transferase (GST); glutathione (GSH); lipid peroxidation (MDA); amino and nucleic acids; SDS- PAGE; RAPD-PCR; Differential Display; Vitamin E; rat; lung; Liver | ||||
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