Molecular Detection of Bacterial Agents of Atypical Pneumonia: Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila in Suez Canal region | ||||
| Medicine Updates | ||||
| Article 11, Volume 6, Issue 6, July 2021, Page 90-115 PDF (1.03 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/muj.2021.75578.1051 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Heba Elsayed 
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| Medical microbiology and immunology, faculty of medicine, Port Said University, Egypt. | ||||
| Abstract | ||||
| Background: Atypical bacterial infections played an important role in community-acquired pneumonia (CAP). It is difficult to detect atypical pathogens by conventional microbiological diagnostic methods. Atypical bacteria do not respond to beta-lactam antibiotics. The use of multiplex polymerase chain reaction (PCR) methods enables rapid and simultaneous detection of many pathogens in a single analysis. Aim: detecting the prevalence of atypical bacterial pathogens as etiologic agents of atypical pneumonia, in the Suez Canal region. Materials and Methods: This cross-sectional descriptive study was conducted throughout 18 months, from October 2018 to April 2020. It included 84 Egyptians suffered from CA atypical pneumonic patients of all age groups from Suez-Canal region, Egypt. Sputum samples were collected for identification of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophilia by using multiplex PCR. Results: Among the 84 atypical pneumonia patients, L. pneumophila were detected in 12 (14%) patients. M. pneumoniae and C. pneumoniae were not detected in our samples. Compared with L. pneumophila -negative cases, L. pneumophila -positive cases were more prevalent in middle aged males, smokers, COPD, diabetic and asthmatic patients (P values = 0.048). Persistent cough, elevated levels of C-reactive protein (C-RP), bilateral pulmonary infiltration are significant clues for predicting L. pneumophila pneumonia. Conclusions: L. pneumophila incidence is not low in our geographical region in atypical pneumonia patients. Clinicians should consider atypical bacterial pathogens while prescribing antimicrobial management plan. Keywords: Atypical pneumonia, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophilia, multiplex-PCR. | ||||
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| Keywords | ||||
| Atypical pneumonia; Mycoplasma pneumoniae; Chlamydophila pneumoniae; Legionella pneumophilia; multiplex-PCR | ||||
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