Biochemical and Histopathological Effects of Colchicine and Moringa oleifera on Thioacetamide-Induced Liver Fibrosis in Rats | ||||
| Egyptian Academic Journal of Biological Sciences, B. Zoology | ||||
| Article 18, Volume 13, Issue 1, June 2021, Page 265-278 PDF (837.32 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsz.2021.180913 | ||||
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| Authors | ||||
| Rasha Tawfike Emara1; Nabil Mohamed Taha1; Abd El-Wahab Ali Mandour1; Mohamed Ali Lebda2; Mohamed Abd Albadia Rashed3; Medhat Mansour Menshawy4 | ||||
| 1Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Egypt | ||||
| 2Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Egypt. | ||||
| 3Biochemistry Department, Animal Health Research Institute, Cairo, Egypt. | ||||
| 4Biology Department, C.B.S and College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, 6th October City, Egypt | ||||
| Abstract | ||||
| Liver fibrosis is the progressive accumulation of connective tissue in the liver, which can be caused by chronic liver injury of various etiologies. Moringa oleifera is a pan-tropical species is used in nurturing both animal and human as an excellent nutritive supplement. Colchicine is a plant that effective against gouty arthritis and other forms of rheumatic diseases (rheumatoid arthritis, familial Mediterranean fever, Bechet´s disease, etc. This study thrown light on the hepatoprotecitve effects of Moringa oleifera ethanolic leaves extract and colchicine on thioacetamide-induced liver fibrosis in rats. Liver function tests (serum ALT, AST, ALP, and Total protein) in addition to histopathological examination of the liver were performed. This study was carried out on a total number of 42 male albino rats were collocated into seven groups (6 rats per group). The experiment lasted for two months, through which all groups except for control and control positive were given Moringa oleifera ethanolic leaves extract and colchicine. Liver fibrosis was induced by intraperitoneal injection of thioacetamide (150 mg/kg) in groups II, V, VI &VII while other groups were injected with 0.9% normal saline of the same volume. Bodyweight was measured three times a week to detect the dose for each rat. The administration of thioacetamide leading a significant increase in serum liver enzyme level ALT, AST, ALP, and Total protein as compared to the control group, while treatment with colchicine and Moringa oleifera ethanolic leaves extract causing significant decreased serum liver enzyme level by (P<0.05) than the induced hepatic group. In general, there is no fundamental difference between groups V, VI, VII. Also revealed that the injection of thioacetamide (150 mg/kg) induced liver fibrosis characterized by a drastic decrease in total protein as compared to the control group by (P <0.05). The colchicine or Moringa oleifera ethanolic leaves extract was able to restore dysfunction. In general, the treatment with colchicine and Moringa oleifera showed good restore in biochemical function and histopathological of the liver. Also, we detected that there are no differences between them. | ||||
| Keywords | ||||
| Moringa oleifera; Colchicine; Liver fibrosis; Biochemical; Histopathological | ||||
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