Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells
Abou-Tarboush, F., El-Ashmaoui, H., Hussein, H., Al-Rajhy, D., Al-Assiry, M. (2005). Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells. EKB Journal Management System, 18(1), 23-28. doi: 10.21608/ejhm.2005.18139
F. M. Abou-Tarboush; H. M. El-Ashmaoui; H. I. Hussein; D. Al-Rajhy; M. Al-Assiry. "Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells". EKB Journal Management System, 18, 1, 2005, 23-28. doi: 10.21608/ejhm.2005.18139
Abou-Tarboush, F., El-Ashmaoui, H., Hussein, H., Al-Rajhy, D., Al-Assiry, M. (2005). 'Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells', EKB Journal Management System, 18(1), pp. 23-28. doi: 10.21608/ejhm.2005.18139
Abou-Tarboush, F., El-Ashmaoui, H., Hussein, H., Al-Rajhy, D., Al-Assiry, M. Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells. EKB Journal Management System, 2005; 18(1): 23-28. doi: 10.21608/ejhm.2005.18139

Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells

The Egyptian Journal of Hospital Medicine
Article 4, Volume 18, Issue 1, January 2005, Page 23-28  XML PDF (294.47 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2005.18139
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Authors
F. M. Abou-Tarboush1; H. M. El-Ashmaoui2; H. I. Hussein3; D. Al-Rajhy3; M. Al-Assiry3
1King Saud University, College of Science, Department of Zoology, P.O. Box 2455, Riyadh 11451, Saudi Arabia
2National Research Center, Cell Biology Dept., Giza, Egypt
3King Saud University, College of Sciences of Foods and Argriculture, Plant Protection Dept, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Abstract
The clastogenic effect of azadirachtin of neemix-4.5 was investigated in SWR/J mouse bone marrow cells. Males and females, 5 each per treatment time, aged 10–12 weeks and weighing 31.7–33.8 g, were orally administrated 9.0 mg/kg (1/10 LD50) of azadirachtin solution. A control group (5 males and 5 females) received only sterile distilled water. The animals were sacrificed 6, 12, 24, 48 or 72 h post- treatment. The chromosome preparations were obtained from bone marrow cells. Chromatid and chromosomal aberrations were investigated in 50 metaphases per animal.
No significant differences in the frequency of chromosomal aberrations or in the percentage of mitotic index were observed between the treated male and female mice at any time intervals used. Hence, data from the two sexes were pooled when analyzed statistically. In the prersent study, the dose level 9.0 mg/kg body weight of azadirachtin of neemix-4.5 did not induce any significant (p>0.05) changes in the percentages of mitotic indices or in chromosomal aberrations in the bone marrow cells of treated animals at all time intervals tested compared with the control group. As the pharmacokinetics of azadirachtin is unknown, the essentially negative results in the present study may be due to a lack of genotoxic potential .
 
Keywords
Neemix-4.5; Azadirachtin; Clastogenic effect; Bone Marrow Cells; Mice
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