Biosynthesis of silver nanoparticles by clove extract to control MDR bacteria isolated from chicken organs 1Ghaly, M. F.; 2Agour M.G.; 1Shaimaa, A., Abbas. 1-Botany and Microbiology Dept., Faculty of Science, Zagazig Uni., Egypt. 2-Animal health research institute, Cairo, Egypt.
Abbas, S., Ghaly, M., Agour, G. (2021). Biosynthesis of silver nanoparticles by clove extract to control MDR bacteria isolated from chicken organs 1Ghaly, M. F.; 2Agour M.G.; 1Shaimaa, A., Abbas. 1-Botany and Microbiology Dept., Faculty of Science, Zagazig Uni., Egypt. 2-Animal health research institute, Cairo, Egypt.. EKB Journal Management System, (), -. doi: 10.21608/bfszu.2021.78837.1076
Shaimaa Abbas; Mohamed Ghaly; Galal Agour. "Biosynthesis of silver nanoparticles by clove extract to control MDR bacteria isolated from chicken organs 1Ghaly, M. F.; 2Agour M.G.; 1Shaimaa, A., Abbas. 1-Botany and Microbiology Dept., Faculty of Science, Zagazig Uni., Egypt. 2-Animal health research institute, Cairo, Egypt.". EKB Journal Management System, , , 2021, -. doi: 10.21608/bfszu.2021.78837.1076
Abbas, S., Ghaly, M., Agour, G. (2021). 'Biosynthesis of silver nanoparticles by clove extract to control MDR bacteria isolated from chicken organs 1Ghaly, M. F.; 2Agour M.G.; 1Shaimaa, A., Abbas. 1-Botany and Microbiology Dept., Faculty of Science, Zagazig Uni., Egypt. 2-Animal health research institute, Cairo, Egypt.', EKB Journal Management System, (), pp. -. doi: 10.21608/bfszu.2021.78837.1076
Abbas, S., Ghaly, M., Agour, G. Biosynthesis of silver nanoparticles by clove extract to control MDR bacteria isolated from chicken organs 1Ghaly, M. F.; 2Agour M.G.; 1Shaimaa, A., Abbas. 1-Botany and Microbiology Dept., Faculty of Science, Zagazig Uni., Egypt. 2-Animal health research institute, Cairo, Egypt.. EKB Journal Management System, 2021; (): -. doi: 10.21608/bfszu.2021.78837.1076

Biosynthesis of silver nanoparticles by clove extract to control MDR bacteria isolated from chicken organs 1Ghaly, M. F.; 2Agour M.G.; 1Shaimaa, A., Abbas. 1-Botany and Microbiology Dept., Faculty of Science, Zagazig Uni., Egypt. 2-Animal health research institute, Cairo, Egypt.

Bulletin of Faculty of Science, Zagazig University
Articles in Press, Accepted Manuscript, Available Online from 04 July 2021  XML
Document Type: Original Article
DOI: 10.21608/bfszu.2021.78837.1076
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Authors
Shaimaa Abbas email 1; Mohamed Ghalyorcid 2; Galal Agour3
1Botany and microbiology, faculty of science, Zagazig university,Egypt
2faculty of science Zagazig univesrsity
3Animal health research institute Cairo Egypt
Abstract
The utilization of antimicrobial drugs has an important effect in chicken husbandry as they are used in prophylaxis, treatment and growth enhancement made poultry a major reservoir of antimicrobial-resistant bacteria. Therefore, our study was undertaken to isolate and control the most common bacterial contamination. For this purpose a total of seventy samples from raw chicken meat and organs (livers, hearts, spleens, muscles, kidneys, skin and gizzards) were collected from different retail markets and abattoirs in Sharkia governorate, Egypt. Nutrient agar was used for the total bacterial count (TBC) , mannitol salt agar for the total Staphylococcus count (TSC) and macConkey’s agar for the total Enterbacteriacae count (TEC). The most common bacteria were identified as Staph. aureus, E.coli and Salmonella isolates in an incidence of 8.57%, 35.7% and 4.28% respectively. The percentage of resistant bacteria to the tested antibiotic were77.8%, 85.7% and 71.4% for Staph. aureus, E.coli and Salmonella respectively. The most effective aquatic plant extract was clove followed by rosemary for its antibacterial effect. The silver nanoparticles has been synthesized and recognized by the color change and UV measurement after incubation of silver nitrate and watery extract of clove at dark conditions, it clearly showed complete inhibitory effect against all the tested MDR bacteria.
Keywords
Staph. aureus; E. coli; salmonella; clove; silver nanoparticles
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