EFFECT OF PGF2 a ON SOME SEMEN CHARACTERS OF CROSS-BRED BULLS (BALADY X FRESIAN) DURING STORAGE AT 4°C | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 22, Volume 40.2, Issue 80, January 1999, Page 310-318 PDF (3.05 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.1999.182491 | ||||
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| Author | ||||
| A.Kh. Abdel-Razek | ||||
| Dept. of Theriogenology Fac. of Vet. Med., Assiut Univ. Head of The Dept. Prof Dr. M.A. El-Naggar | ||||
| Abstract | ||||
| at 4°C for 4 days was Incubation of bull spermatozoa with PGF2 a performed to investigate the effect of PG on some sperm cell characters and enzymatic release. Semen from cross-bred bulls (Balady X Fresian) was diluted with egg yolk citrate at a rate of 1:10. Aliquots were supplemented with PGF2 a in levels of 0, 200, 400 and 600 ug ml and stored at 4°C for 4 days. Daily examination for each treatment was carried during the storage period. The percentage of sperm motility (SM%), alive sperm (AS%) and sperm with intact acrosome (IA%) w decreased significantly with doses higher than 200 ug ml. In the same time, abnormal sperm percentage (AbS%) increased in samples which contain 400 and 600 ug ml. Continuous increase in transaminases activities in the extra-cellular medium were noticed with the increase level of PGF2 a and prolongation of storage time. The results suggested that, addition of PGF2 a with high concentration (more than 200 ugl ml) to bull spermatozoa during storage at°C adversely affect sperm motility, viability and abnormalities as well as induced membrane damage and impair permeability. | ||||
| Keywords | ||||
| Key Words: PGF 2a; Bull semen; Extension; Invitro | ||||
| References | ||||
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CLARIAS GARIEPINUS | ||||
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