ANTIGEN CAPTURE ELISA TECHNIQUE FOR RAPID DETECTION OF SALMONELLA TYPHIMURIUM IN FAECAL SAMPLES OF DIARRHOEIC COW CALVES. | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 25, Volume 39.2, Issue 78, July 1998, Page 324-333 PDF (3.43 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.1998.183219 | ||||
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| Authors | ||||
| E.M. Riad; A.I. Tanios; A.F. Abd El-Moghny | ||||
| Animal Health Research Institute, Dokki, Giza, Egypt. | ||||
| Abstract | ||||
| A total of 66 faecal samples was collected from cow calves suffering from profuse watery diarrhoea and examined by conventional cultural method and antigen capture ELISA. Twelve strains of Salmonella typhimurium were isolated in a percentage of 18.2 % by culture method while 16 samples were detected as positive for Salmonella infection with a percentage of 24.2 % by antigen capture ELISA. Comparison of two 324 Assiut Vet. Med. J. Vol. 39 No. 78, July 1998. pre-enrichment procedures by ELISA test revealed that 13 samples were positive with tetrathionate broth and subjected directly to ELISA test with a percentage of 19.7 %, while 16 samples were positive using M broth after tetrathionate enrichment with a percentage of 24.2 %. Antigen capture ELISA was rapid and sensitive for identification of Salmonella infected animals. | ||||
| Keywords | ||||
| Key Words: Elisa; Salmonella Typhimurium; Diarrhoea; Calves | ||||
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