TRIALS OF USING CELL CULTURES FOR EVALUATION OF IBDV VACCINES IN EGYPT | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 7, Volume 36.2, Issue 72, January 1997, Page 105-123 PDF (4.47 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.1997.183346 | ||||
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| Authors | ||||
| ELHAM, A. EL-EBIARY; SUSAN, S. EL-MAHDY; M. H. KHODEIR | ||||
| Abstract | ||||
| Three cell cultures were used in this work to propagate and replicate the infectious bursal disease virus (IBDV), (primary chicken embryo fibroblast cells "CEF" and two cell lines, African green monkey kidney cells (VERO) and baby hamster kidney cell (BHK) and compare their sensitivity to that of embryonated chicken eggs in order to use the selected cell culture adapted IBDV in the routine serum neutralization test (SNT) and evaluate its immunogenicity and pathogenicity in chicken. It was found that: 1- The cytopathic effect (CPE) of the IBDV in CEF and BHK was similar where it began to be pronounced after 9 passages at 2 days post inoculation (PI) and took 3 days in both cell culture. The cell culture adapted IBDV lost its infectivity to the embryonated chicken egg at the passage No. 5 as after the passage No. 2 the EID 50 was 102.2 per 0.1 ml. The peak virus titre (105 TCID50 / 0.1 ml) was detected at 54 and 60 hours post inoculation in BHK and CEF respectively, while the virus titre in VERO cells reached a plateau 105 TCID50 / 0.1 ml at 60 hours PI. 2- The detection of the level of antibody titre in the sera of vaccinated chicken, using the microtitre neutralization test and embryonated chicken eggs (ECE) revealed comparable results. 3- These preliminary studies indicated that the cell culture adapted IBDV could be used safely as a vaccinal strain in the production of cell culture vaccine, as it gave a good immunity when compared with the egg adapted imported vaccine. | ||||
| Keywords | ||||
| Key words: IBDV; vaccines; cell culture; Egypt | ||||
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