Indirect Spectrophotometric Estimation of Ciprofloxacin Hydrochloride in Pharmaceuticals using N-Bromosuccinimide and Methylene Blue Dye | ||||
| Egyptian Journal of Chemistry | ||||
| Article 23, Volume 65, Issue 1, January 2022, Page 207-214 PDF (543.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2021.79851.3930 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mahasin A. Hussein; Khalida M. Omar | ||||
| Department of Chemistry/ College of Science/ University of Mosul/ Iraq | ||||
| Abstract | ||||
| The present study aims to develop a swift , simple, accurate and sensitive spectrophotometric method for determination of Ciprofloxacin Hydrochloride ( CPFX ) in pure and pharmaceutical formulations. The suggested method depend on oxidation of CPFX with known excess amount of N-bromosuccinimide ( NBS ) in acidic medium and after reaction is insured to be complete , the surplus NBS is estimated by de colorization of methylene blue dye and measuring the absorbance of surplus dye at 665 nm . A linear calibration curve was obtained over the concentration range 0.5 – 4.5 μg.ml-1 with correlation coefficient of 0.9993 . The molar absorptivity and sandell's sensitivity index values were determined to be 8.6 ×104 L.mol-1.cm-1 and 0.004276 µg.cm-2, respectively .The limit of detection (LOD) and quantification (LOQ) were calculated to be 0.0033 and 0.0111 μg.ml-1, respectively . The proposed method has been successfully applied to the determination of CIPH in available dosage form, the validity proposed method was confirmed by recovery study via standard addition technique . | ||||
| Keywords | ||||
| Ciprofloxacin Hydrochloride; N-bromosuccinimide; Determination; Spectrophotometry; Methylene blue | ||||
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