New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes
Azab, H., Khairy, G., Abd El-Ghany, N., Ahmed, M. (2016). New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes. EKB Journal Management System, 15(1), 69-76.
Hassan Azab; Gasser Khairy; N. Abd El-Ghany; Marwa Ahmed. "New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes". EKB Journal Management System, 15, 1, 2016, 69-76.
Azab, H., Khairy, G., Abd El-Ghany, N., Ahmed, M. (2016). 'New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes', EKB Journal Management System, 15(1), pp. 69-76.
Azab, H., Khairy, G., Abd El-Ghany, N., Ahmed, M. New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes. EKB Journal Management System, 2016; 15(1): 69-76.

New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes

Catrina: The International Journal of Environmental Sciences
Article 7, Volume 15, Issue 1, 2016, Page 69-76  XML PDF (858.22 K)
Document Type: Original Article
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Authors
Hassan Azab1; Gasser Khairy2; N. Abd El-Ghany; Marwa Ahmed email 3
1Chemistry Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt.
2Chemistry Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt. Chemistry Department, Faculty of Science and Arts, Aljouf University, P.O. Box # 2014, Skaka-41421, Kingdom of Saudi Arabia.
3Chemistry Department, Faculty of Science, Suez Canal University, El-Arish, Egyp
Abstract
The luminescence arising from lanthanide cations offers several advantages over organic fluorescent molecules sharp, distinctive emission bands allow for easy resolution between multiple lanthanide signals; long emission lifetimes (μs–ms) make them excellent candidates for time resolved measurements and high resistance to photo bleaching allow for long or repeated experiments. A developed method is presented for determination of nucleobase, nucleosides and nucleotides using the effect of quenching of fluorescence of the easily accessible terbium (III) - 8-allyl-2-oxo-2H-chromene-3-carbaldehyde (AOCC) complex in 1:2 metals: ligand ratio. The interaction of terbium (III) - 8-allyl-2-oxo-2H-chromene-3-carbaldehyde (AOCC) complex with nucleosides (NS) (cytidine, inosine), nucleobase (NB) (adenine), nucleotides (NT) (5′-GMP), (5′-IMP), (5′- ATP), (5′-CMP) and (5′-ADP) has been studied using normal luminescence techniques. The linear ranges for determination of the selected biomolecules are 1.6–36.6 μ mol.dm-3, and with limit of detection were in the range of 0.906- 8.28μ mol.dm-3.
Keywords
Fluorescence; Nucleotides; Nucleobases; Nucleosides; Lanthanide complexes; time-resolved
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