DOT ENZYME LINKED IMMUNOSORBENT ASSAY AS A QUICK RELIABLE DIAGNOSTIC AID FOR LEPTOSPIROSIS | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 18, Volume 36.1, Issue 71, October 1996, Page 167-172 PDF (1.51 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.1996.184005 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| E.R.H. ATTIA; I. G.A. IBRAHIM; M.S. TAWFIK | ||||
| Abstract | ||||
| Two hundred cowserum samples were collected from infertile cases at private farms in Egypt. These samples were serologically screened by Microscopic Aglutination Test (MAT), Macroscopic Agglutination Test and Dot ELISA for detection of leptospiral antibodies. The obtained results revealed that antibodies against L.hardjo, L.grippotyphosa and L.icterohaemorrhagiae were detected in a percentages of (6%), (5%) and (1%) respectively by using MAT and Dot ELISA, while (12.5%), 7.5%) and (2.5%) respectively by using Macroscopic Agglutination Test. It can be concluded that MAT, Dot ELISA and Macroscopic Agglutination Test can be used to screen leptospiral infection in cattle. However, Dot ELISA was easy,rapid and highly sensitive to detect leptospiral antibodies in cattle with positive titre at 1: 200 and more. | ||||
| Keywords | ||||
| Key words: Leptospirosis; Diagnosis; Dot ELISA | ||||
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