DIRECT DETECTION OF MYCOPLASMA GALLISEPICUM (S6 STRAIN) BY POLYMERASE CHAIN REACTION (PCR) | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 10, Volume 32.2, Issue 64, January 1995, Page 102-109 PDF (1.91 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.1995.184027 | ||||
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| Authors | ||||
| S.A.A. EL-SHATER; ELHAM A. EBIARY; S.A. ESSIA; NAHLA, R.E.K | ||||
| Abstract | ||||
| The polymerase chain reaction was used for rapid detection and differentiation between different strains of Mycoplasmagallisepticum. Two oligonucleotide primers of 22 and 23 base pair in length were constructed. The PCR amplification products were visualized by ethidium bromide and ultraviolet exposure. The results were as follows: 1. A positive single PCR product 960 bp in size was detected for Mycoplasma gallisepticum (56 strains). 2. Other strains of mycoplasma (oral, arginini, salivarium and pneumonia) showed no band at 960 bp molecular weight. This technique provides a simple and extremely sensitive method of identifing isolates of M. gallisepticum and other Mycoplasmas. | ||||
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